Yu Song, Gang Li, Jacqueline Nowak, Xiaoqing Zhang, Dongbei Xu, Xiujuan Yang, Guoqiang Huang, Wanqi Liang, Litao Yang, Canhua Wang, Vincent Bulone, Zoran Nikoloski, Jianping Hu, Staffan Persson, Dabing Zhang
- Light and gravity are two key determinants in orientating plant stems for proper growth and development. The organization and dynamics of the actin cytoskeleton are essential for cell biology and critically regulated by actin-binding proteins. However, the role of actin cytoskeleton in shoot negative gravitropism remains controversial. In this work, we report that the actin-binding protein Rice Morphology Determinant (RMD) promotes reorganization of the actin cytoskeleton in rice (Oryza sativa) shoots. The changes in actin organization are associated with the ability of the rice shoots to respond to negative gravitropism. Here, light-grown rmd mutant shoots exhibited agravitropic phenotypes. By contrast, etiolated rmd shoots displayed normal negative shoot gravitropism. Furthermore, we show that RMD maintains an actin configuration that promotes statolith mobility in gravisensing endodermal cells, and for proper auxin distribution in light-grown, but not dark-grown, shoots. RMD gene expression is diurnally controlled and directlyLight and gravity are two key determinants in orientating plant stems for proper growth and development. The organization and dynamics of the actin cytoskeleton are essential for cell biology and critically regulated by actin-binding proteins. However, the role of actin cytoskeleton in shoot negative gravitropism remains controversial. In this work, we report that the actin-binding protein Rice Morphology Determinant (RMD) promotes reorganization of the actin cytoskeleton in rice (Oryza sativa) shoots. The changes in actin organization are associated with the ability of the rice shoots to respond to negative gravitropism. Here, light-grown rmd mutant shoots exhibited agravitropic phenotypes. By contrast, etiolated rmd shoots displayed normal negative shoot gravitropism. Furthermore, we show that RMD maintains an actin configuration that promotes statolith mobility in gravisensing endodermal cells, and for proper auxin distribution in light-grown, but not dark-grown, shoots. RMD gene expression is diurnally controlled and directly repressed by the phytochrome-interacting factor-like protein OsPIL16. Consequently, overexpression of OsPIL16 led to gravisensing and actin patterning defects that phenocopied the rmd mutant. Our findings outline a mechanism that links light signaling and gravity perception for straight shoot growth in rice.…
MetadatenAuthor details: | Yu Song, Gang Li, Jacqueline NowakORCiDGND, Xiaoqing ZhangORCiD, Dongbei Xu, Xiujuan Yang, Guoqiang Huang, Wanqi LiangORCiD, Litao Yang, Canhua Wang, Vincent Bulone, Zoran NikoloskiORCiDGND, Jianping Hu, Staffan PerssonORCiDGND, Dabing Zhang |
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DOI: | https://doi.org/10.1104/pp.19.00497 |
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ISSN: | 0032-0889 |
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ISSN: | 1532-2548 |
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Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/31416828 |
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Title of parent work (English): | Plant physiology : an international journal devoted to physiology, biochemistry, cellular and molecular biology, biophysics and environmental biology of plants |
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Publisher: | American Society of Plant Physiologists |
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Place of publishing: | Rockville |
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Publication type: | Article |
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Language: | English |
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Year of first publication: | 2019 |
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Publication year: | 2019 |
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Release date: | 2020/11/23 |
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Volume: | 181 |
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Issue: | 2 |
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Number of pages: | 15 |
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First page: | 630 |
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Last Page: | 644 |
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Funding institution: | National Key Research and Development Program of China [2016YFD0100804]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [31430009]; Innovative Research Team, Ministry of Education [B14016]; 111 ProjectMinistry of Education, China - 111 Project [B14016]; Science and Technology Commission of Shanghai MunicipalityScience & Technology Commission of Shanghai Municipality (STCSM) [13JC1408200]; China Scholarship Council (CSC)China Scholarship Council [201506230050]; Australian Research Council Future FellowshipAustralian Research Council [FT160100218]; University of Melbourne International Research and Research Training Fund-Research Network and Consortia |
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Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
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DDC classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
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Peer review: | Referiert |
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Publishing method: | Open Access |
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| Open Access / Bronze Open-Access |
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