Mapping of Multiple Complementary Sex Determination Loci in a Parasitoid Wasp
- Sex determination has evolved in a variety of ways and can depend on environmental and genetic signals. A widespread form of genetic sex determination is haplodiploidy, where unfertilized, haploid eggs develop into males and fertilized diploid eggs into females. One of the molecular mechanisms underlying haplodiploidy in Hymenoptera, the large insect order comprising ants, bees, and wasps, is complementary sex determination (CSD). In species with CSD, heterozygosity at one or several loci induces female development. Here, we identify the genomic regions putatively underlying multilocus CSD in the parasitoid wasp Lysiphlebus fabarum using restriction -site associated DNA sequencing. By analyzing segregation patterns at polymorphic sites among 331 diploid males and females, we identify up to four CSD candidate regions, all on different chromosomes. None of the candidate regions feature evidence for homology with the csd gene from the honey bee, the only species in which CSD has been characterized, suggesting that CSD in L. fabarum isSex determination has evolved in a variety of ways and can depend on environmental and genetic signals. A widespread form of genetic sex determination is haplodiploidy, where unfertilized, haploid eggs develop into males and fertilized diploid eggs into females. One of the molecular mechanisms underlying haplodiploidy in Hymenoptera, the large insect order comprising ants, bees, and wasps, is complementary sex determination (CSD). In species with CSD, heterozygosity at one or several loci induces female development. Here, we identify the genomic regions putatively underlying multilocus CSD in the parasitoid wasp Lysiphlebus fabarum using restriction -site associated DNA sequencing. By analyzing segregation patterns at polymorphic sites among 331 diploid males and females, we identify up to four CSD candidate regions, all on different chromosomes. None of the candidate regions feature evidence for homology with the csd gene from the honey bee, the only species in which CSD has been characterized, suggesting that CSD in L. fabarum is regulated via a novel molecular mechanism. Moreover, no homology is shared between the candidate loci, in contrast to the idea that multilocus CSD should emerge from duplications of an ancestral single -locus system. Taken together, our results suggest that the molecular mechanisms underlying CSD in Hymenoptera are not conserved between species, raising the question as to whether CSD may have evolved multiple times independently in the group.…
Author details: | Cyril Matthey-DoretORCiD, Casper J. van der KooiORCiD, Daniel L. JeffriesORCiD, Jens BastORCiD, Alice B. DennisORCiD, Christoph VorburgerORCiDGND, Tanja SchwanderORCiD |
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DOI: | https://doi.org/10.1093/gbe/evz219 |
ISSN: | 1759-6653 |
Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/31596478 |
Title of parent work (English): | Genome biology and evolution |
Publisher: | Oxford Univ. Press |
Place of publishing: | Oxford |
Publication type: | Article |
Language: | English |
Date of first publication: | 2019/10/09 |
Publication year: | 2019 |
Release date: | 2020/10/30 |
Tag: | CSD; Lysiphlebus fabarum; hymenoptera; sex determination |
Volume: | 11 |
Issue: | 10 |
Number of pages: | 9 |
First page: | 2954 |
Last Page: | 2962 |
Funding institution: | Swiss National Science FoundationSwiss National Science Foundation (SNSF) [PP00P3_139013, PP00P3_170627]; SNSFSwiss National Science Foundation (SNSF) [PP00P3_146341]; Sinergia grant [CRSII3_154396] |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
DDC classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Peer review: | Referiert |
Publishing method: | Open Access |
Open Access / Gold Open-Access | |
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