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Iconographic evidence from Egypt suggests that watermelon pulp was consumed there as a dessert by 4,360 BP.
Earlier archaeobotanical evidence comes from seeds from Neolithic settlements in Libya, but whether these were watermelons with sweet pulp or other forms is unknown.
We generated genome sequences from 6,000- and 3,300-year-old seeds from Libya and Sudan, and from worldwide herbarium collections made between 1824 and 2019, and analyzed these data together with resequenced genomes from important germplasm collections for a total of 131 accessions.
Phylogenomic and population-genomic analyses reveal that (1) much of the nuclear genome of both ancient seeds is traceable to West African seed-use "egusi-type" watermelon (Citrullus mucosospermus) rather than domesticated pulp-use watermelon (Citrullus lanatus ssp. vulgaris); (2) the 6,000-year-old watermelon likely had bitter pulp and greenish-white flesh as today found in C. mucosospermus, given alleles in the bitterness regulators ClBT and in the red color marker LYCB; and (3) both ancient genomes showed admixture from C. mucosospermus, C. lanatus ssp. cordophanus, C. lanatus ssp. vulgaris, and even South African Citrullus amarus, and evident introgression between the Libyan seed (UMB-6) and populations of C. lanatus.
An unexpected new insight is that Citrullus appears to have initially been collected or cultivated for its seeds, not its flesh, consistent with seed damage patterns induced by human teeth in the oldest Libyan material.
Background:
Inflammaging is considered to drive loss of muscle function. Omega-3 fatty acids exhibit anti-inflammatory properties. Therefore, we examined the effects of eight weeks of vibration and home-based resistance exercise combined with a whey-enriched, omega-3-supplemented diet on muscle power, inflammation and muscle biomarkers in community-dwelling old adults.
Methods:
Participants were randomized to either exercise (3x/week, n = 20), exercise + high-protein diet (1.2-1.5 g/kg, n = 20), or exercise + high-protein and omega-3-enriched diet (2.2 g/day, n = 21). Muscle power (watt/m(2)) and chair rise test (CRT) time (s) were assessed via CRT measured with mechanography. Furthermore, leg strength (kg/m(2)) and fasting concentrations of inflammatory (interleukin (IL-) 6, IL-10, high-mobility group box-1 (HMGB-1)) and muscle biomarkers (insulin-like growth factor (IGF-) 1, IGF-binding protein-3, myostatin) were assessed.
Results:
Sixty-one participants (70.6 +/- 4.7 years; 47% men) completed the study. According to generalized linear mixed models, a high-protein diet improved leg strength and CRT time. Only IGF-1 increased with additional omega-3. Sex-specific analyses revealed that muscle power, IL-6, IL-6/IL-10 ratio, and HMGB-1 improved significantly in the male high-protein, omega-3-enriched group only.
Conclusion:
Vibration and home-based resistance exercise combined with a high-protein, omega-3-enriched diet increased muscle power and reduced inflammation in old men, but not in old women. While muscle biomarkers remained unchanged, a high-protein diet combined with exercise improved leg strength and CRT time.
Age-depth relationships are the key elements in paleoenvironmental studies to place proxy measurements into a temporal context.
However, potential influencing factors of the available radiocarbon data and the associated modeling process can cause serious divergences of age-depth relationships from true chronologies, which is particularly challenging for paleolimnological studies in Arctic regions.
This paper provides geoscientists with a tool-assisted approach to compare outputs from age-depth modeling systems and to strengthen the robustness of age-depth relationships.
We primarily focused on the development of age determination data from a data collection of high-latitude lake systems (50 to 90 circle N, 55 sediment cores, and a total of 602 dating points). Our approach used five age-depth modeling systems (Bacon, Bchron, clam, hamstr, Undatable) that we linked through a multi-language Jupyter Notebook called LANDO ("Linked age and depth modeling").
Within LANDO we implemented a pipeline from data integration to model comparison to allow users to investigate the outputs of the modeling systems.
In this paper, we focused on highlighting three different case studies: comparing multiple modeling systems for one sediment core with a continuously deposited succession of dating points (CS1), for one sediment core with scattered dating points (CS2), and for multiple sediment cores (CS3).
For the first case study (CS1), we showed how we facilitate the output data from all modeling systems to create an ensemble age-depth model. In the special case of scattered dating points (CS2), we introduced an adapted method that uses independent proxy data to assess the performance of each modeling system in representing lithological changes. Based on this evaluation, we reproduced the characteristics of an existing age-depth model (Lake Ilirney, EN18208) without removing age determination data.
For multiple sediment cores (CS3) we found that when considering the Pleistocene-Holocene transition, the main regime changes in sedimentation rates do not occur synchronously for all lakes.
We linked this behavior to the uncertainty within the dating and modeling process, as well as the local variability in catchment settings affecting the accumulation rates of the sediment cores within the collection near the glacial-interglacial transition.
The growing number of wheat-related allergies worldwide has resulted in a new trend towards gluten-free alternatives. In this context, alternative cereals such as sorghum and oats are attracting new interest. Given the limited data available, the question of whether these cereals are completely safe and gluten-free for allergy sufferers remains open. One of the key steps in protein research is their efficient extraction. In this work, the Osborne sequential extraction method was developed and optimized using the response surface methodology in order to fractionate oat proteins. An optimized desirability of 0.986 was achieved with an extraction time of 4.7 min, a speed of 6, and a sample/solvent ratio of 5. The corresponding optimized responses were 8.7, 4.0, and 5.1% for the extraction yields of the avenin, avenalin, and albumin/globulin fractions, respectively. Further characterization of the extracts was carried out on 24 homogeneous and commercial oat samples via LC-MS/MS, targeting six potentially allergenic proteins. The avenin-E protein featured prominently, with relative contents of 60.7, 32.2, 58.0, and 59.8% in the total extract, avenin, avenalin, and albumin/globulin fractions, respectively, while the Avenin-3, ATI-2, avenin, SSG2, and SSG1 proteins in the total extract showed levels of 16.4, 9.3, 6.6, 4.8, and 2.2%, respectively. The preliminary results of an ELISA performed on the different fractions revealed low levels of gluten (from 1.24 ± 0.14 to 3.61 ± 0.16 mg/kg), which were well below the threshold limit of 20 mg/kg. These results support the hypothesis that oats can be a safe food for people suffering from cereal-related allergies. These results open the door to further studies into the comprehensive characterization of oat proteins.
Subjective and objective difficulty of emotional facial expression perception from dynamic stimuli
(2022)
This study aimed to discover predictors of subjective and objective difficulty in emotion perception from dynamic facial expressions.
We used a multidimensional emotion perception framework, in which observers rated the perceived emotion along a number of dimensions instead of choosing from traditionally-used discrete categories of emotions.
Data were collected online from 441 participants who rated facial expression stimuli in a novel paradigm designed to separately measure subjective (self-reported) and objective (deviation from the population consensus) difficulty.
We targeted person-specific (sex and age of observers and actors) and stimulus-specific (valence and arousal values) predictors of those difficulty scores.
Our findings suggest that increasing age of actors makes emotion perception more difficult for observers, and that perception difficulty is underestimated by men in comparison to women, and by younger and older adults in comparison to middle-aged adults.
The results also yielded an increase in the objective difficulty measure for female observers and female actors.
Stimulus-specific factors-valence and arousal-exhibited quadratic relationships with subjective and objective difficulties: very positive and very negative stimuli were linked to reduced subjective and objective difficulty, whereas stimuli of very low and high arousal were linked to decreased subjective but increased objective difficulty. Exploratory analyses revealed low relevance of person-specific variables for the prediction of difficulty but highlighted the importance of valence in emotion perception, in line with functional accounts of emotions.
Our findings highlight the need to complement traditional emotion recognition paradigms with novel designs, like the one presented here, to grasp the "big picture" of human emotion perception.
In situ U-Th-Pb dating of parisite: implication for the age of mineralization of Colombian emeralds
(2022)
Parisite-Ce (Ca(Ce,La)(2)(CO3)(3)F-2) is a rare-earth (REE) fluorocarbonate mineral first described from the world-famous emerald mines of the Muzo district, Boyaca Province, Colombia.
Four samples of parisite-Ce collected from outcrops near Muzo have been geochemically studied and dated using the in situ laser ablation U-Th-Pb method.
Our study shows that the REE abundance of parisite is controlled by the leaching of the wall rocks (black schist). Furthermore, we show that the parisite-Ce crystals formed in textural equilibrium with the emeralds, suggesting a similar time of crystallization.
Our analysis demonstrates the capability of parisite as a geochronometer and shows that precise and accurate U-Th-Pb ages can be obtained from parisite after common Pb-207 correction. A higher precision date was obtained with the Th-Pb ratio rather than with the U-Pb ratio because of the relatively higher content of Th than U in the samples. The samples yielded Th-208-Pb-232 ages ranging from similar to 47 to 51 Ma. The new ages are similar to 10 Ma older than previously reported Ar-Ar ages and similar to 10 Ma younger than previously reported Rb/Sr ages.
These results will have significant implications for understanding the timing of mineralization and crystallization of emerald deposits in Colombia.
Furthermore, this study opens new avenues for dating similar deposits worldwide.
River flooding has large societal and economic impacts across Africa.
Despite the importance of this topic, little is known about the main flood generating mechanisms in Africa. This study is based on 13,815 flood events that occurred between 1981 and 2018 in 529 catchments.
These flood events are classified to identify the different flood drivers: excess rains, long rains and short rains.
Out of them, excess rains on saturated soils in Western Africa, and long rains for catchments in Northern and Southern Africa, are the two dominant mechanisms, contributing to more than 75% of all flood events.
The aridity index is strongly related to the spatial repartition of the different flood generating processes showing the climatic controls on floods.
Few significant changes were detected in the relative importance of these drivers over time, but the rather short time series available prevent a robust assessment of flood driver changes in most catchments.
The major implication of these results is to underline the importance of soil moisture dynamics, in addition to rainfall, to analyze the evolution of flood hazards in Africa.
Ground subsidence and erosion processes caused by permafrost thaw pose a high risk to infrastructure in the Arctic. Climate warming is increasingly accelerating the thawing of permafrost, emphasizing the need for thorough monitoring to detect damages and hazards at an early stage. The use of unoccupied aerial vehicles (UAVs) allows a fast and uncomplicated analysis of sub-meter changes across larger areas compared to manual surveys in the field. In our study, we investigated the potential of photogrammetry products derived from imagery acquired with off-the-shelf UAVs in order to provide a low-cost assessment of the risks of permafrost degradation along critical infrastructure. We tested a minimal drone setup without ground control points to derive high-resolution 3D point clouds via structure from motion (SfM) at a site affected by thermal erosion along the Dalton Highway on the North Slope of Alaska. For the sub-meter change analysis, we used a multiscale point cloud comparison which we improved by applying (i) denoising filters and (ii) alignment procedures to correct for horizontal and vertical offsets. Our results show a successful reduction in outliers and a thorough correction of the horizontal and vertical point cloud offset by a factor of 6 and 10, respectively. In a defined point cloud subset of an erosion feature, we derive a median land surface displacement of -0.35 m from 2018 to 2019. Projecting the development of the erosion feature, we observe an expansion to NNE, following the ice-wedge polygon network. With a land surface displacement of -0.35 m and an alignment root mean square error of 0.99 m, we find our workflow is best suitable for detecting and quantifying rapid land surface changes. For a future improvement of the workflow, we recommend using alternate flight patterns and an enhancement of the point cloud comparison algorithm.
This paper reviews data collection practices in electromagnetic articulography (EMA) studies, with a focus on sensor placement. We first introduce electromagnetic articulography as a method. We then focus on existing data collection practices. Our overview is based on a literature review of 905 publications from a large variety of journals and conferences, identified through a systematic keyword search in Google Scholar. The review shows that experimental designs vary greatly, which in turn may limit researchers' ability to compare results across studies. Finally, we describe an EMA data collection procedure that includes an articulatory-driven strategy for determining where to position sensors on the tongue without causing discomfort to the participant. We also evaluate three approaches for preparing (NDI Wave) EMA sensors reported in the literature with respect to the duration the sensors remain attached to the tongue: 1) attaching out-of-the-box sensors, 2) attaching sensors coated in latex, and 3) attaching sensors coated in latex with an additional latex flap. Results indicate no clear general effect of sensor preparation type on adhesion duration. A subsequent exploratory analysis reveals that sensors with the additional flap tend to adhere for shorter times than the other two types, but that this pattern is inverted for the most posterior tongue sensor.
We present a novel method of temporal modulation of X-ray radiation for time resolved experiments. To control the intensity of the X-ray beam, the Bragg reflection of a piezoelectric crystal is modified using comb-shaped electrodes deposited on the crystal surface. Voltage applied to the electrodes induces a periodic deformation of the crystal that acts as a diffraction grating, splitting the original Bragg reflection into several satellites. A pulse of X-rays can be created by rapidly switching the voltage on and off. In our prototype device the duty cycle was limited to similar to 1 ns by the driving electronics. The prototype can be used to generate X-ray pulses from a continuous source. It can also be electrically correlated to a synchrotron light source and be activated to transmit only selected synchrotron pulses. Since the device operates in a non-resonant mode, different activation patterns and pulse durations can be achieved. Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License.