Electrochemical MIP Sensor for Butyrylcholinesterase
- Molecularly imprinted polymers (MIPs) mimic the binding sites of antibodies by substituting the amino acid-scaffold of proteins by synthetic polymers. In this work, the first MIP for the recognition of the diagnostically relevant enzyme butyrylcholinesterase (BuChE) is presented. The MIP was prepared using electropolymerization of the functional monomer o-phenylenediamine and was deposited as a thin film on a glassy carbon electrode by oxidative potentiodynamic polymerization. Rebinding and removal of the template were detected by cyclic voltammetry using ferricyanide as a redox marker. Furthermore, the enzymatic activity of BuChE rebound to the MIP was measured via the anodic oxidation of thiocholine, the reaction product of butyrylthiocholine. The response was linear between 50 pM and 2 nM concentrations of BuChE with a detection limit of 14.7 pM. In addition to the high sensitivity for BuChE, the sensor responded towards pseudo-irreversible inhibitors in the lower mM range.
Author details: | Goksu OzcelikayORCiD, Sevinc KurbanogluORCiD, Xiaorong ZhangGND, Çağla Kosak SözORCiD, Ulla WollenbergerORCiDGND, Sibel A. OzkanORCiD, Aysu YarmanORCiDGND, Frieder W. SchellerORCiDGND |
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DOI: | https://doi.org/10.3390/polym11121970 |
ISSN: | 2073-4360 |
Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/31801184 |
Title of parent work (English): | Polymers |
Publisher: | MDPI |
Place of publishing: | Basel |
Publication type: | Article |
Language: | English |
Year of first publication: | 2019 |
Publication year: | 2019 |
Release date: | 2020/09/22 |
Tag: | biomimetic sensors; butyrylcholinesterase; molecularly imprinted polymers; o-phenylenediamine; rivastigmine |
Volume: | 11 |
Issue: | 12 |
Number of pages: | 11 |
Funding institution: | ERA-Chemistry (2014) [61133]; Deutsche Forschungsgemeinschaft (DFG, Research Foundation (DFG) [EXC 2008/1, 390540038]; Ankara University Erasmus Program; Ankara University UBED ProgramAnkara University |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
DDC classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Peer review: | Referiert |
Publishing method: | Open Access |
Open Access / Gold Open-Access | |
DOAJ gelistet | |
External remark: | Zweitveröffentlichung in der Schriftenreihe Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe ; 1138 |