Specific functions for mediator complex subunits from different modules in the transcriptional response of arabidopsis thaliana to abiotic stress
- Adverse environmental conditions are detrimental to plant growth and development. Acclimation to abiotic stress conditions involves activation of signaling pathways which often results in changes in gene expression via networks of transcription factors (TFs). Mediator is a highly conserved co-regulator complex and an essential component of the transcriptional machinery in eukaryotes. Some Mediator subunits have been implicated in stress-responsive signaling pathways; however, much remains unknown regarding the role of plant Mediator in abiotic stress responses. Here, we use RNA-seq to analyze the transcriptional response of Arabidopsis thaliana to heat, cold and salt stress conditions. We identify a set of common abiotic stress regulons and describe the sequential and combinatorial nature of TFs involved in their transcriptional regulation. Furthermore, we identify stress-specific roles for the Mediator subunits MED9, MED16, MED18 and CDK8, and putative TFs connecting them to different stress signaling pathways. Our data also indicateAdverse environmental conditions are detrimental to plant growth and development. Acclimation to abiotic stress conditions involves activation of signaling pathways which often results in changes in gene expression via networks of transcription factors (TFs). Mediator is a highly conserved co-regulator complex and an essential component of the transcriptional machinery in eukaryotes. Some Mediator subunits have been implicated in stress-responsive signaling pathways; however, much remains unknown regarding the role of plant Mediator in abiotic stress responses. Here, we use RNA-seq to analyze the transcriptional response of Arabidopsis thaliana to heat, cold and salt stress conditions. We identify a set of common abiotic stress regulons and describe the sequential and combinatorial nature of TFs involved in their transcriptional regulation. Furthermore, we identify stress-specific roles for the Mediator subunits MED9, MED16, MED18 and CDK8, and putative TFs connecting them to different stress signaling pathways. Our data also indicate different modes of action for subunits or modules of Mediator at the same gene loci, including a co-repressor function for MED16 prior to stress. These results illuminate a poorly understood but important player in the transcriptional response of plants to abiotic stress and identify target genes and mechanisms as a prelude to further biochemical characterization.…
Author details: | Tim CrawfordORCiD, Fazeelat KaramatORCiD, Nóra Lehotai, Matilda RentoftORCiD, Jeanette Blomberg, Åsa StrandORCiD, Stefan BjörklundORCiD |
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DOI: | https://doi.org/10.1038/s41598-020-61758-w |
ISSN: | 2045-2322 |
Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/32193425 |
Title of parent work (English): | Scientific reports |
Publisher: | Macmillan Publishers Limited, part of Springer Nature |
Place of publishing: | London |
Publication type: | Article |
Language: | English |
Date of first publication: | 2020/03/19 |
Publication year: | 2020 |
Release date: | 2023/03/23 |
Tag: | MED16; activation; circadian clock; defense; jasmonate; network; plant Mediator; regulate gene expression; salicylic-acid; signal transduction |
Volume: | 10 |
Issue: | 1 |
Article number: | 5073 |
Number of pages: | 18 |
First page: | 1 |
Last Page: | 18 |
Funding institution: | Knut and Alice Wallenberg Foundation Knut & Alice Wallenberg Foundation; [2015-0056]; Swedish Foundation for Strategic ResearchSwedish Foundation; for Strategic Research [SB16-0089]; Swedish Research CouncilSwedish; Research CouncilEuropean Commission [201603943, 2016-04319]; Umea; University |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
DDC classification: | 5 Naturwissenschaften und Mathematik / 50 Naturwissenschaften / 500 Naturwissenschaften und Mathematik |
5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie | |
6 Technik, Medizin, angewandte Wissenschaften / 60 Technik / 600 Technik, Technologie | |
Peer review: | Referiert |
Publishing method: | Open Access / Gold Open-Access |
DOAJ gelistet | |
License (German): | CC-BY - Namensnennung 4.0 International |
External remark: | Zweitveröffentlichung in der Schriftenreihe Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe ; 1399 |