Disruption of an RNA-binding hinge region abolishes LHP1-mediated epigenetic repression
- Epigenetic maintenance of gene repression is essential for development. Polycomb complexes are central to this memory, but many aspects of the underlying mechanism remain unclear. LIKE HETEROCHROMATIN PROTEIN 1 (LHP1) binds Polycomb-deposited H3K27me3 and is required for repression of many Polycomb target genes in Arabidopsis. Here we show that LHP1 binds RNA in vitro through the intrinsically disordered hinge region. By independently perturbing the RNA-binding hinge region and H3K27me3 (trimethylation of histone H3 at Lys27) recognition, we found that both facilitate LHP1 localization and H3K27me3 maintenance. Disruption of the RNAbinding hinge region also prevented formation of subnuclear foci, structures potentially important for epigenetic repression.
| Author details: | Scott BerryORCiD, Stefanie RosaGND, Martin HowardORCiD, Marc Buhler, Caroline Dean |
|---|---|
| DOI: | https://doi.org/10.1101/gad.305227.117 |
| ISSN: | 0890-9369 |
| ISSN: | 1549-5477 |
| Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/29212661 |
| Title of parent work (English): | Genes & Development |
| Publisher: | Cold Spring Harbor Laboratory Press |
| Place of publishing: | Cold Spring Harbor, NY |
| Publication type: | Article |
| Language: | English |
| Date of first publication: | 2017/11/07 |
| Publication year: | 2017 |
| Release date: | 2021/02/09 |
| Tag: | Polycomb; RNA; chromatin; epigenetics; plant biology |
| Volume: | 31 |
| Number of pages: | 6 |
| First page: | 2115 |
| Last Page: | 2120 |
| Funding institution: | European Research Council Advanced Investigator Grant MEXTIM; UK Biotechnology and Biological Sciences Research Council Institute Strategic Programme [BB/J004588/1]; EMBO Short-Term Fellowship; John Innes Foundation |
| Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
| DDC classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
| Peer review: | Referiert |
| Publishing method: | Open Access / Gold Open-Access |
| License (German): |  CC-BY - Namensnennung 4.0 International |
