Development of a molecularly imprinted polymer-based electrochemical sensor for tyrosinase
- For the first time a molecularly imprinted polymer (MIP)-based sensor for tyrosinase is described. This sensor is based on the electropolymerization of scopoletin or o-phenylenediamine in the presence of tyrosinase from mushrooms, which has a high homology to the human enzyme. The template was removed either by treatment with proteinase Kor by alkaline treatment. The measuring signal was generated either by measuring the formation of a product by the target enzyme or by evaluation of the permeability of the redox marker ferricyanide. The o-phenylenediamine-based MIP sensor has a linear measuring range up to 50 nM of tyrosinase with a limit of detection of 3.97 nM (R 2 = 0.994) and shows good discrimination towards other proteins, e.g., bovine serum albumin and cytochrome c.
| Author details: | Aysu YarmanORCiDGND |
|---|---|
| DOI: | https://doi.org/10.3906/kim-1708-68 |
| ISSN: | 1300-0527 |
| Title of parent work (English): | Turkish journal of chemistry |
| Publisher: | Türkiye Bilimsel ve Teknik Araştırma Kurumu |
| Place of publishing: | Ankara |
| Publication type: | Article |
| Language: | English |
| Date of first publication: | 2017/11/06 |
| Publication year: | 2017 |
| Release date: | 2022/03/31 |
| Tag: | Molecularly imprinted polymers; biomimetic sensors; electropolymerization; ophenylenediamine; scopoletin; tyrosinase |
| Volume: | 42 |
| Issue: | 2 |
| Number of pages: | 9 |
| First page: | 346 |
| Last Page: | 354 |
| Funding institution: | Deutsche Forschungsgemeinschaft (DFG)German Research Foundation (DFG) [EXC 314]; Turkish-German University Scientific Research Projects Commission [2016BF0011] |
| Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
| DDC classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
| Peer review: | Referiert |
| Publishing method: | Open Access / Bronze Open-Access |
