Broadening the scope of sortagging
- Sortases are enzymes occurring in the cell wall of Gram-positive bacteria. Sortase A (SrtA), the best studied sortase class, plays a key role in anchoring surface proteins with the recognition sequence LPXTG covalently to oligoglycine units of the bacterial cell wall. This unique transpeptidase activity renders SrtA attractive for various purposes and motivated researchers to study multiple in vivo and in vitro ligations in the last decades. This ligation technique is known as sortase-mediated ligation (SML) or sortagging and developed to a frequently used method in basic research. The advantages are manifold: extremely high substrate specificity, simple access to substrates and enzyme, robust nature and easy handling of sortase A. In addition to the ligation of two proteins or peptides, early studies already included at least one artificial (peptide equipped) substrate into sortagging reactions - which demonstrates the versatility and broad applicability of SML. Thus, SML is not only a biology-related technique, but has foundSortases are enzymes occurring in the cell wall of Gram-positive bacteria. Sortase A (SrtA), the best studied sortase class, plays a key role in anchoring surface proteins with the recognition sequence LPXTG covalently to oligoglycine units of the bacterial cell wall. This unique transpeptidase activity renders SrtA attractive for various purposes and motivated researchers to study multiple in vivo and in vitro ligations in the last decades. This ligation technique is known as sortase-mediated ligation (SML) or sortagging and developed to a frequently used method in basic research. The advantages are manifold: extremely high substrate specificity, simple access to substrates and enzyme, robust nature and easy handling of sortase A. In addition to the ligation of two proteins or peptides, early studies already included at least one artificial (peptide equipped) substrate into sortagging reactions - which demonstrates the versatility and broad applicability of SML. Thus, SML is not only a biology-related technique, but has found prominence as a major interdisciplinary research tool. In this review, we provide an overview about the use of sortase A in interdisciplinary research, mainly for protein modification, synthesis of protein-polymer conjugates and immobilization of proteins on surfaces.…
Author details: | Xiaolin Dai, Alexander BökerORCiDGND, Ulrich GlebeORCiDGND |
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DOI: | https://doi.org/10.1039/c8ra06705h |
ISSN: | 2046-2069 |
Title of parent work (English): | RSC Advances |
Publisher: | Royal Society of Chemistry |
Place of publishing: | Cambridge |
Publication type: | Review |
Language: | English |
Date of first publication: | 2019/02/06 |
Publication year: | 2019 |
Release date: | 2021/04/08 |
Volume: | 9 |
Issue: | 9 |
Number of pages: | 22 |
First page: | 4700 |
Last Page: | 4721 |
Funding institution: | Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)German Research Foundation (DFG) [414977640]; Fraunhofer High Performance Center for Functional Integration in Materials |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Physik und Astronomie |
DDC classification: | 5 Naturwissenschaften und Mathematik / 53 Physik / 530 Physik |
Peer review: | Referiert |
Publishing method: | Open Access / Gold Open-Access |
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License (English): | Creative Commons - Namensnennung-Nicht kommerziell 3.0 Unported |