Susanne Dunker, Matthew Boyd, Walter Durka, Silvio Erler, W. Stanley Harpole, Silvia Henning, Ulrike Herzschuh, Thomas Hornick, Tiffany Knight, Stefan Lips, Patrick Mäder, Elena Motivans Švara, Steven Mozarowski, Demetra Rakosy, Christine Römermann, Mechthild Schmitt-Jansen, Kathleen Stoof-Leichsenring, Frank Stratmann, Regina Treudler, Risto Virtanen, Katrin Wendt-Potthoff, Christian Wilhelm
- Environmental monitoring involves the quantification of microscopic cells and particles such as algae, plant cells, pollen, or fungal spores. Traditional methods using conventional microscopy require expert knowledge, are time-intensive and not well-suited for automated high throughput. Multispectral imaging flow cytometry (MIFC) allows measurement of up to 5000 particles per second from a fluid suspension and can simultaneously capture up to 12 images of every single particle for brightfield and different spectral ranges, with up to 60x magnification. The high throughput of MIFC has high potential for increasing the amount and accuracy of environmental monitoring, such as for plant-pollinator interactions, fossil samples, air, water or food quality that currently rely on manual microscopic methods. Automated recognition of particles and cells is also possible, when MIFC is combined with deep-learning computational techniques. Furthermore, various fluorescence dyes can be used to stain specific parts of the cell to highlightEnvironmental monitoring involves the quantification of microscopic cells and particles such as algae, plant cells, pollen, or fungal spores. Traditional methods using conventional microscopy require expert knowledge, are time-intensive and not well-suited for automated high throughput. Multispectral imaging flow cytometry (MIFC) allows measurement of up to 5000 particles per second from a fluid suspension and can simultaneously capture up to 12 images of every single particle for brightfield and different spectral ranges, with up to 60x magnification. The high throughput of MIFC has high potential for increasing the amount and accuracy of environmental monitoring, such as for plant-pollinator interactions, fossil samples, air, water or food quality that currently rely on manual microscopic methods. Automated recognition of particles and cells is also possible, when MIFC is combined with deep-learning computational techniques. Furthermore, various fluorescence dyes can be used to stain specific parts of the cell to highlight physiological and chemical features including: vitality of pollen or algae, allergen content of individual pollen, surface chemical composition (carbohydrate coating) of cells, DNA- or enzyme-activity staining. Here, we outline the great potential for MIFC in environmental research for a variety of research fields and focal organisms. In addition, we provide best practice recommendations.…
MetadatenAuthor details: | Susanne Dunker, Matthew Boyd, Walter DurkaORCiD, Silvio Erler, W. Stanley Harpole, Silvia Henning, Ulrike HerzschuhORCiDGND, Thomas Hornick, Tiffany KnightORCiD, Stefan LipsORCiD, Patrick MäderORCiD, Elena Motivans Švara, Steven Mozarowski, Demetra Rakosy, Christine Römermann, Mechthild Schmitt-Jansen, Kathleen Stoof-Leichsenring, Frank Stratmann, Regina Treudler, Risto VirtanenORCiD, Katrin Wendt-Potthoff, Christian Wilhelm |
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DOI: | https://doi.org/10.1002/cyto.a.24658 |
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ISSN: | 1552-4922 |
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ISSN: | 1552-4930 |
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Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/35670307 |
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Title of parent work (English): | Cytometry part A |
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Publisher: | Wiley |
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Place of publishing: | Hoboken |
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Publication type: | Article |
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Language: | English |
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Date of first publication: | 2022/06/07 |
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Publication year: | 2022 |
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Release date: | 2024/07/10 |
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Tag: | environmental monitoring; imaging flow cytometry; plant traits |
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Volume: | 101 |
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Issue: | 9 |
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Number of pages: | 18 |
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First page: | 782 |
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Last Page: | 799 |
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Funding institution: | Bundesministerium fur Bildung und Forschung [02WPL1448A];; Bundesministerium fur Ernahrung und Landwirtschaft [2819NA066,; 2819NA102, 2819NA106]; Deutsche Forschungsgemeinschaft [34600830-13,; 34600865-16, RA-373/20] |
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Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
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| Mathematisch-Naturwissenschaftliche Fakultät / Institut für Umweltwissenschaften und Geographie |
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Peer review: | Referiert |
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Publishing method: | Open Access / Hybrid Open-Access |
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License (German): | CC-BY-NC - Namensnennung, nicht kommerziell 4.0 International |
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