Spontaneous transitions between amoeboid and keratocyte-like modes of migration
- The motility of adherent eukaryotic cells is driven by the dynamics of the actin cytoskeleton. Despite the common force-generating actin machinery, different cell types often show diverse modes of locomotion that differ in their shape dynamics, speed, and persistence of motion. Recently, experiments in Dictyostelium discoideum have revealed that different motility modes can be induced in this model organism, depending on genetic modifications, developmental conditions, and synthetic changes of intracellular signaling. Here, we report experimental evidence that in a mutated D. discoideum cell line with increased Ras activity, switches between two distinct migratory modes, the amoeboid and fan-shaped type of locomotion, can even spontaneously occur within the same cell. We observed and characterized repeated and reversible switchings between the two modes of locomotion, suggesting that they are distinct behavioral traits that coexist within the same cell. We adapted an established phenomenological motility model that combines aThe motility of adherent eukaryotic cells is driven by the dynamics of the actin cytoskeleton. Despite the common force-generating actin machinery, different cell types often show diverse modes of locomotion that differ in their shape dynamics, speed, and persistence of motion. Recently, experiments in Dictyostelium discoideum have revealed that different motility modes can be induced in this model organism, depending on genetic modifications, developmental conditions, and synthetic changes of intracellular signaling. Here, we report experimental evidence that in a mutated D. discoideum cell line with increased Ras activity, switches between two distinct migratory modes, the amoeboid and fan-shaped type of locomotion, can even spontaneously occur within the same cell. We observed and characterized repeated and reversible switchings between the two modes of locomotion, suggesting that they are distinct behavioral traits that coexist within the same cell. We adapted an established phenomenological motility model that combines a reaction-diffusion system for the intracellular dynamics with a dynamic phase field to account for our experimental findings.…
Author details: | Ted MoldenhawerORCiD, Eduardo Moreno, Daniel SchindlerORCiDGND, Sven FlemmingORCiD, Matthias HolschneiderORCiDGND, Wilhelm HuisingaORCiDGND, Sergio AlonsoORCiDGND, Carsten BetaORCiDGND |
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DOI: | https://doi.org/10.3389/fcell.2022.898351 |
ISSN: | 2296-634X |
Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/36247011 |
Title of parent work (English): | Frontiers in Cell and Developmental Biology |
Publisher: | Frontiers Media |
Place of publishing: | Lausanne |
Publication type: | Article |
Language: | English |
Date of first publication: | 2022/09/30 |
Publication year: | 2022 |
Release date: | 2023/10/10 |
Tag: | D. discoideum; actin dynamics; amoeboid motility; cell migration; keratocytle-like motility; migration; modes of |
Volume: | 10 |
Article number: | 898351 |
Number of pages: | 13 |
Funding institution: | Deutsche Forschungsgemeinschaft (DFG); MCIN/AEI [318763901-SFB1294];; "ERDF A way of making Europe" [PGC 2018-095456-b-I00]; European Union |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Physik und Astronomie |
DDC classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Peer review: | Referiert |
Publishing method: | Open Access / Gold Open-Access |
DOAJ gelistet | |
License (German): | CC-BY - Namensnennung 4.0 International |