Aaron P. Seitz, Fabian Schumacher, Jennifer Baker, Matthias Soddemann, Barbara Wilker, Charles C. Caldwell, Ryan M. Gobble, Markus Kamler, Katrin Anne Becker, Sascha Beck, Burkhard Kleuser, Michael J. Edwards, Erich Gulbins
- Ventilator-associated pneumonia (VAP) is a major cause of morbidity and mortality in critically ill patients. Here, we employed the broad antibacterial effects of sphingosine to prevent VAP by developing a novel method of coating surfaces of endotracheal tubes with sphingosine and sphingosine analogs. Sphingosine and phytosphingosine coatings of endotracheal tubes prevent adherence and mediate killing of Pseudomonas aeruginosa, Acinetobacter baumannii, and Staphylococcus aureus, even in biofilms. Most importantly, sphingosine-coating of endotracheal tubes also prevented P. aeruginosa and S. aureus pneumonia in vivo. Coating of the tubes with sphingosine was stable, without obvious side effects on tracheal epithelial cells and did not induce inflammation. In summary, we describe a novel method to coat plastic surfaces and provide evidence for the application of sphingosine and phytosphingosine as novel antimicrobial coatings to prevent bacterial adherence and induce killing of pathogens on the surface of endotracheal tubes withVentilator-associated pneumonia (VAP) is a major cause of morbidity and mortality in critically ill patients. Here, we employed the broad antibacterial effects of sphingosine to prevent VAP by developing a novel method of coating surfaces of endotracheal tubes with sphingosine and sphingosine analogs. Sphingosine and phytosphingosine coatings of endotracheal tubes prevent adherence and mediate killing of Pseudomonas aeruginosa, Acinetobacter baumannii, and Staphylococcus aureus, even in biofilms. Most importantly, sphingosine-coating of endotracheal tubes also prevented P. aeruginosa and S. aureus pneumonia in vivo. Coating of the tubes with sphingosine was stable, without obvious side effects on tracheal epithelial cells and did not induce inflammation. In summary, we describe a novel method to coat plastic surfaces and provide evidence for the application of sphingosine and phytosphingosine as novel antimicrobial coatings to prevent bacterial adherence and induce killing of pathogens on the surface of endotracheal tubes with potential to prevent biofilm formation and VAP.Key messagesNovel dip-coating method to coat plastic surfaces with lipids.Sphingosine and phytosphingosine as novel antimicrobial coatings on plastic surface.Sphingosine coatings of endotracheal tubes prevent bacterial adherence and biofilms.Sphingosine coatings of endotracheal tubes induce killing of pathogens.Sphingosine coatings of endotracheal tubes ventilator-associated pneumonia.…
MetadatenAuthor details: | Aaron P. Seitz, Fabian SchumacherORCiDGND, Jennifer Baker, Matthias Soddemann, Barbara Wilker, Charles C. Caldwell, Ryan M. Gobble, Markus Kamler, Katrin Anne BeckerORCiDGND, Sascha Beck, Burkhard KleuserORCiDGND, Michael J. Edwards, Erich GulbinsORCiDGND |
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DOI: | https://doi.org/10.1007/s00109-019-01800-1 |
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ISSN: | 0946-2716 |
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ISSN: | 1432-1440 |
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Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/31222488 |
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Title of parent work (English): | Journal of molecular medicine |
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Publisher: | Springer |
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Place of publishing: | Heidelberg |
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Publication type: | Article |
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Language: | English |
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Date of first publication: | 2019/06/20 |
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Publication year: | 2019 |
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Release date: | 2020/12/08 |
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Tag: | Acinetobacter baumannii; Coating; Plastic surfaces; Pseudomonas aeruginosa; Sphingosine; Staphylococcus aureus; Ventilation |
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Volume: | 97 |
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Issue: | 8 |
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Number of pages: | 17 |
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First page: | 1195 |
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Last Page: | 1211 |
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Funding institution: | DFG-grantGerman Research Foundation (DFG) [Gu 335-34/1, GRK 2098]; United States Air ForceUnited States Department of Defense [AF-FA8650-14-2-6B33]; U.S. Air Force School of Aerospace Medicine [FA8650-10-2-6140] |
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Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
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DDC classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
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Peer review: | Referiert |
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Publishing method: | Open Access / Hybrid Open-Access |
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