Diel Variations of Extracellular Microcystin Influence the Subcellular Dynamics of RubisCO in Microcystis aeruginosa PCC 7806
- The ubiquitous freshwater cyanobacterium Microcystis is remarkably successful, showing a high tolerance against fluctuations in environmental conditions. It frequently forms dense blooms which can accumulate significant amounts of the hepatotoxin microcystin, which plays an extracellular role as an infochemical but also acts intracellularly by interacting with proteins of the carbon metabolism, notably with the CO2 fixing enzyme RubisCO. Here we demonstrate a direct link between external microcystin and its intracellular targets. Monitoring liquid cultures of Microcystis in a diel experiment revealed fluctuations in the extracellular microcystin content that correlate with an increase in the binding of microcystin to intracellular proteins. Concomitantly, reversible relocation of RubisCO from the cytoplasm to the cell’s periphery was observed. These variations in RubisCO localization were especially pronounced with cultures grown at higher cell densities. We replicated these effects by adding microcystin externally to cultures grownThe ubiquitous freshwater cyanobacterium Microcystis is remarkably successful, showing a high tolerance against fluctuations in environmental conditions. It frequently forms dense blooms which can accumulate significant amounts of the hepatotoxin microcystin, which plays an extracellular role as an infochemical but also acts intracellularly by interacting with proteins of the carbon metabolism, notably with the CO2 fixing enzyme RubisCO. Here we demonstrate a direct link between external microcystin and its intracellular targets. Monitoring liquid cultures of Microcystis in a diel experiment revealed fluctuations in the extracellular microcystin content that correlate with an increase in the binding of microcystin to intracellular proteins. Concomitantly, reversible relocation of RubisCO from the cytoplasm to the cell’s periphery was observed. These variations in RubisCO localization were especially pronounced with cultures grown at higher cell densities. We replicated these effects by adding microcystin externally to cultures grown under continuous light. Thus, we propose that microcystin may be part of a fast response to conditions of high light and low carbon that contribute to the metabolic flexibility and the success of Microcystis in the field.…
Verfasserangaben: | Arthur GuljamowORCiDGND, Tino BarchewitzORCiDGND, Rebecca Große, Stefan TimmORCiDGND, Martin HagemannORCiDGND, Elke DittmannORCiDGND |
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DOI: | https://doi.org/10.3390/microorganisms9061265 |
ISSN: | 2076-2607 |
Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/34200971 |
Titel des übergeordneten Werks (Englisch): | Microorganisms : open access journal |
Verlag: | MDPI |
Verlagsort: | Basel |
Publikationstyp: | Wissenschaftlicher Artikel |
Sprache: | Englisch |
Datum der Erstveröffentlichung: | 10.05.2021 |
Erscheinungsjahr: | 2021 |
Titel verleihende Institution: | Universität Potsdam |
Datum der Freischaltung: | 15.10.2021 |
Freies Schlagwort / Tag: | Microcystis; RubisCO; cyanobacterial bloom; extracellular signaling; microcystin |
Band: | 9 |
Ausgabe: | 6 |
Aufsatznummer: | 1265 |
Seitenanzahl: | 14 |
Fördernde Institution: | Universität Potsdam |
Fördernde Institution: | Deutsche Forschungsgemeinschaft (DFG) |
Fördernde Institution: | DFG German Research Foundation (DFG), European Commission |
Fördernde Institution: | HBFG program |
Fördernummer: | PA 2021_057 |
Fördernummer: | Di910/10-1, Ha2002/20-1 |
Fördernummer: | SFB 1127 |
Fördernummer: | GZ: INST 264/125-1 FUGG |
Organisationseinheiten: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
DDC-Klassifikation: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Peer Review: | Referiert |
Fördermittelquelle: | Publikationsfonds der Universität Potsdam |
Publikationsweg: | Open Access / Gold Open-Access |
Lizenz (Deutsch): | CC-BY - Namensnennung 4.0 International |
Externe Anmerkung: | Zweitveröffentlichung in der Schriftenreihe Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe ; 1154 |