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Gaining insight into plant gene transcription using smFISH

  • Single molecule RNA fluorescent in situ hybridization (smFISH) enables gene transcription to be assessed at the cellular level. In this point of view article, we describe our recent smFISH research in the model plant Arabidopsis thaliana and discuss how this technique could further knowledge of plant gene transcription in the future.

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Author details:Susan DuncanORCiD, Stefanie Nunes Rosa
DOI:https://doi.org/10.1080/21541264.2017.1372043
ISSN:2154-1264
ISSN:2154-1272
Pubmed ID:https://pubmed.ncbi.nlm.nih.gov/28990856
Title of parent work (English):Transcription
Publisher:Taylor & Francis Group
Place of publishing:Philadelphia
Publication type:Review
Language:English
Date of first publication:2017/11/03
Publication year:2017
Release date:2022/03/25
Tag:Arabidopsis; RNA Imaging; lncRNA; mRNA Quantification; smFISH
Volume:9
Issue:3
Number of pages:5
First page:166
Last Page:170
Funding institution:Humboldt Foundation (Germany)Alexander von Humboldt Foundation [BB/L014130/1, 3.3-GRO/1162118STP]; BBSRC, Core Strategic Programme at the Earlham Institute [BB/CSP17270/1]
Organizational units:Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie
DDC classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Peer review:Referiert
Publishing method:Open Access / Bronze Open-Access
License (German):License LogoCC-BY-NC-ND - Namensnennung, nicht kommerziell, keine Bearbeitungen 4.0 International
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