Jia Xuan Leong, Margot Raffeiner, Daniela Spinti, Gautier Langin, Mirita Franz-Wachtel, Andrew R. Guzman, Jung-Gun Kim, Pooja Pandey, Alyona E. Minina, Boris Macek, Anders Hafren, Tolga O. Bozkurt, Mary Beth Mudgett, Frederik Börnke, Daniel Hofius, Suayib Uestuen
- Beyond its role in cellular homeostasis, autophagy plays anti- and promicrobial roles in host-microbe interactions, both in animals and plants.
One prominent role of antimicrobial autophagy is to degrade intracellular pathogens or microbial molecules, in a process termed xenophagy.
Consequently, microbes evolved mechanisms to hijack or modulate autophagy to escape elimination.
Although well-described in animals, the extent to which xenophagy contributes to plant-bacteria interactions remains unknown.
Here, we provide evidence that Xanthomonas campestris pv. vesicatoria (Xcv) suppresses host autophagy by utilizing type-III effector XopL. XopL interacts with and degrades the autophagy component SH3P2 via its E3 ligase activity to promote infection.
Intriguingly, XopL is targeted for degradation by defense-related selective autophagy mediated by NBR1/Joka2, revealing a complex antagonistic interplay between XopL and the host autophagy machinery.
Our results implicate plant antimicrobial autophagy in the depletion of aBeyond its role in cellular homeostasis, autophagy plays anti- and promicrobial roles in host-microbe interactions, both in animals and plants.
One prominent role of antimicrobial autophagy is to degrade intracellular pathogens or microbial molecules, in a process termed xenophagy.
Consequently, microbes evolved mechanisms to hijack or modulate autophagy to escape elimination.
Although well-described in animals, the extent to which xenophagy contributes to plant-bacteria interactions remains unknown.
Here, we provide evidence that Xanthomonas campestris pv. vesicatoria (Xcv) suppresses host autophagy by utilizing type-III effector XopL. XopL interacts with and degrades the autophagy component SH3P2 via its E3 ligase activity to promote infection.
Intriguingly, XopL is targeted for degradation by defense-related selective autophagy mediated by NBR1/Joka2, revealing a complex antagonistic interplay between XopL and the host autophagy machinery.
Our results implicate plant antimicrobial autophagy in the depletion of a bacterial virulence factor and unravel an unprecedented pathogen strategy to counteract defense-related autophagy in plant-bacteria interactions.…
MetadatenAuthor details: | Jia Xuan LeongORCiD, Margot RaffeinerORCiD, Daniela Spinti, Gautier Langin, Mirita Franz-Wachtel, Andrew R. Guzman, Jung-Gun Kim, Pooja Pandey, Alyona E. Minina, Boris Macek, Anders Hafren, Tolga O. Bozkurt, Mary Beth Mudgett, Frederik BörnkeORCiDGND, Daniel Hofius, Suayib Uestuen |
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DOI: | https://doi.org/10.15252/embj.2021110352 |
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ISSN: | 1460-2075 |
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Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/35620914 |
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Title of parent work (English): | The EMBO journal |
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Publisher: | Wiley |
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Place of publishing: | Hoboken |
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Publication type: | Article |
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Language: | English |
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Date of first publication: | 2022/05/27 |
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Publication year: | 2022 |
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Release date: | 2024/06/14 |
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Tag: | autophagy; effectors; immunity; ubiquitination; xenophagy |
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Volume: | 41 |
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Issue: | 13 |
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Article number: | e110352 |
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Number of pages: | 17 |
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Funding institution: | Deutsche Forschungsgemeinschaft (DFG) [GZ: UE188/2-1, SFB1101]; EU; [799433]; DFG [BO1961/5-2, INST 37/819-1 FUGG, INST 37/965-1 FUGG];; Swedish research councils VR [2016-04562, 2020-05327]; FORMAS; [2017-01596, 2016-01044]; BBSRC - Biotechnology and Biological Sciences; Research Council [BB/T006102/1]; NSF IOS Grant [2026368]; Projekt DEAL |
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Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
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DDC classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
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Peer review: | Referiert |
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Publishing method: | Open Access / Hybrid Open-Access |
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| DOAJ gelistet |
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License (German): | CC-BY - Namensnennung 4.0 International |
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