Lei Yang, Valentina Perrera, Eleftheria Saplaoura, Federico Apelt, Mathieu Bahin, Amira Kramdi, Justyna Jadwiga Olas, Bernd Müller-Röber, Ewelina Sokolowska, Wenna Zhang, Runsheng Li, Nicolas Pitzalis, Manfred Heinlein, Shoudong Zhang, Auguste Genovesio, Vincent Colot, Friedrich Kragler
- In plants, transcripts move to distant body parts to potentially act as systemic signals regulating development and growth. Thousands of messenger RNAs (mRNAs) are transported across graft junctions via the phloem to distinct plant parts. Little is known regarding features, structural motifs, and potential base modifications of transported transcripts and how these may affect their mobility. We identified Arabidopsis thalianam RNAs harboring the modified base 5-methylcytosine (m(5)C) and found that these are significantly enriched in mRNAs previously described as mobile, moving over graft junctions to distinct plant parts. We confirm this finding with graft-mobile methylated mRNAs TRANSLATIONALLY CONTROLLED TUMOR PROTEIN 1 (TCTP1) and HEAT SHOCK COGNATE PROTEIN 70.1 (HSC70.1), whose mRNA transport is diminished in mutants deficient in m(5)C mRNA methylation. Together, our results point toward an essential role of cytosine methylation in systemic mRNA mobility in plants and that TCTP1 mRNA mobility is required for its signalingIn plants, transcripts move to distant body parts to potentially act as systemic signals regulating development and growth. Thousands of messenger RNAs (mRNAs) are transported across graft junctions via the phloem to distinct plant parts. Little is known regarding features, structural motifs, and potential base modifications of transported transcripts and how these may affect their mobility. We identified Arabidopsis thalianam RNAs harboring the modified base 5-methylcytosine (m(5)C) and found that these are significantly enriched in mRNAs previously described as mobile, moving over graft junctions to distinct plant parts. We confirm this finding with graft-mobile methylated mRNAs TRANSLATIONALLY CONTROLLED TUMOR PROTEIN 1 (TCTP1) and HEAT SHOCK COGNATE PROTEIN 70.1 (HSC70.1), whose mRNA transport is diminished in mutants deficient in m(5)C mRNA methylation. Together, our results point toward an essential role of cytosine methylation in systemic mRNA mobility in plants and that TCTP1 mRNA mobility is required for its signaling function.…
MetadatenAuthor details: | Lei Yang, Valentina Perrera, Eleftheria Saplaoura, Federico ApeltORCiDGND, Mathieu Bahin, Amira Kramdi, Justyna Jadwiga OlasORCiDGND, Bernd Müller-RöberORCiDGND, Ewelina SokolowskaORCiD, Wenna Zhang, Runsheng LiORCiD, Nicolas Pitzalis, Manfred Heinlein, Shoudong ZhangORCiD, Auguste Genovesio, Vincent Colot, Friedrich KraglerORCiD |
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DOI: | https://doi.org/10.1016/j.cub.2019.06.042 |
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ISSN: | 0960-9822 |
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ISSN: | 1879-0445 |
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Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/31327714 |
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Title of parent work (English): | Current biology |
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Publisher: | Cell Press |
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Place of publishing: | Cambridge |
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Publication type: | Article |
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Language: | English |
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Year of first publication: | 2019 |
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Publication year: | 2019 |
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Release date: | 2020/12/02 |
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Volume: | 29 |
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Issue: | 15 |
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Number of pages: | 17 |
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First page: | 2465 |
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Last Page: | 2476.e5 |
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Funding institution: | [ANR-10-LABX-54 MEMO LIFE, ANR-11-IDEX-0001-02 PSL*]; DFGGerman Research Foundation (DFG) [CRC 973]; MPI-MPP internal funds; Chinese Scholarship CouncilChina Scholarship Council; EMBO Long-Term FellowshipEuropean Molecular Biology Organization (EMBO); IMPRS; ERC-Syg PLAMORF grant |
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Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
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DDC classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
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Peer review: | Referiert |
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Publishing method: | Open Access / Hybrid Open-Access |
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