Development of a molecularly imprinted polymer-based electrochemical sensor for tyrosinase
- For the first time a molecularly imprinted polymer (MIP)-based sensor for tyrosinase is described. This sensor is based on the electropolymerization of scopoletin or o-phenylenediamine in the presence of tyrosinase from mushrooms, which has a high homology to the human enzyme. The template was removed either by treatment with proteinase Kor by alkaline treatment. The measuring signal was generated either by measuring the formation of a product by the target enzyme or by evaluation of the permeability of the redox marker ferricyanide. The o-phenylenediamine-based MIP sensor has a linear measuring range up to 50 nM of tyrosinase with a limit of detection of 3.97 nM (R 2 = 0.994) and shows good discrimination towards other proteins, e.g., bovine serum albumin and cytochrome c.
| Verfasserangaben: | Aysu YarmanORCiDGND |
|---|---|
| DOI: | https://doi.org/10.3906/kim-1708-68 |
| ISSN: | 1300-0527 |
| Titel des übergeordneten Werks (Englisch): | Turkish journal of chemistry |
| Verlag: | Türkiye Bilimsel ve Teknik Araştırma Kurumu |
| Verlagsort: | Ankara |
| Publikationstyp: | Wissenschaftlicher Artikel |
| Sprache: | Englisch |
| Datum der Erstveröffentlichung: | 06.11.2017 |
| Erscheinungsjahr: | 2017 |
| Datum der Freischaltung: | 31.03.2022 |
| Freies Schlagwort / Tag: | Molecularly imprinted polymers; biomimetic sensors; electropolymerization; ophenylenediamine; scopoletin; tyrosinase |
| Band: | 42 |
| Ausgabe: | 2 |
| Seitenanzahl: | 9 |
| Erste Seite: | 346 |
| Letzte Seite: | 354 |
| Fördernde Institution: | Deutsche Forschungsgemeinschaft (DFG)German Research Foundation (DFG) [EXC 314]; Turkish-German University Scientific Research Projects Commission [2016BF0011] |
| Organisationseinheiten: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
| DDC-Klassifikation: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
| Peer Review: | Referiert |
| Publikationsweg: | Open Access / Bronze Open-Access |
