Emina Halilbasic, Elisabeth Fuerst, Denise Heiden, Lukasz Japtok, Susanne C. Diesner, Michael Trauner, Askin Kulu, Peter Jaksch, Konrad Hoetzenecker, Burkhard Kleuser, Lili Kazemi-Shirazi, Eva Untersmayr
- Recent research has linked sphingolipid (SL) metabolism with cystic fibrosis transmembrane conductance regulator (CFTR) activity, affecting bioactive lipid mediator sphingosine-1-phosphate (S1P). We hypothesize that loss of CFTR function in cystic fibrosis (CF) patients influenced plasma S1P levels. Total and unbound plasma S1P levels were measured in 20 lung-transplanted adult CF patients and 20 healthy controls by mass spectrometry and enzyme-linked immunosorbent assay (ELISA). S1P levels were correlated with CFTR genotype, routine laboratory parameters, lung function and pathogen colonization, and clinical symptoms. Compared to controls, CF patients showed lower unbound plasma S1P, whereas total S1P levels did not differ. A positive correlation of total and unbound S1P levels was found in healthy controls, but not in CF patients. Higher unbound S1P levels were measured in Delta F508-homozygous compared to Delta F508-heterozygous CF patients (p = 0.038), accompanied by higher levels of HDL in Delta F508-heterozygous patients.Recent research has linked sphingolipid (SL) metabolism with cystic fibrosis transmembrane conductance regulator (CFTR) activity, affecting bioactive lipid mediator sphingosine-1-phosphate (S1P). We hypothesize that loss of CFTR function in cystic fibrosis (CF) patients influenced plasma S1P levels. Total and unbound plasma S1P levels were measured in 20 lung-transplanted adult CF patients and 20 healthy controls by mass spectrometry and enzyme-linked immunosorbent assay (ELISA). S1P levels were correlated with CFTR genotype, routine laboratory parameters, lung function and pathogen colonization, and clinical symptoms. Compared to controls, CF patients showed lower unbound plasma S1P, whereas total S1P levels did not differ. A positive correlation of total and unbound S1P levels was found in healthy controls, but not in CF patients. Higher unbound S1P levels were measured in Delta F508-homozygous compared to Delta F508-heterozygous CF patients (p = 0.038), accompanied by higher levels of HDL in Delta F508-heterozygous patients. Gastrointestinal symptoms were more common in Delta F508 heterozygotes compared to Delta F508 homozygotes. This is the first clinical study linking plasma S1P levels with CFTR function and clinical presentation in adult CF patients. Given the emerging role of immunonutrition in CF, our study might pave the way for using S1P as a novel biomarker and nutritional target in CF.…
MetadatenVerfasserangaben: | Emina HalilbasicORCiD, Elisabeth Fuerst, Denise Heiden, Lukasz JaptokGND, Susanne C. Diesner, Michael TraunerORCiD, Askin Kulu, Peter JakschORCiD, Konrad Hoetzenecker, Burkhard KleuserORCiDGND, Lili Kazemi-Shirazi, Eva UntersmayrORCiD |
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DOI: | https://doi.org/10.3390/nu12030765 |
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ISSN: | 2072-6643 |
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Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/32183316 |
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Titel des übergeordneten Werks (Englisch): | Nutrients |
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Untertitel (Englisch): | potential target for immunonutrition? |
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Verlag: | MDPI |
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Verlagsort: | Basel |
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Publikationstyp: | Wissenschaftlicher Artikel |
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Sprache: | Englisch |
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Datum der Erstveröffentlichung: | 14.03.2020 |
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Erscheinungsjahr: | 2020 |
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Datum der Freischaltung: | 25.05.2023 |
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Freies Schlagwort / Tag: | Delta F508 mutation; cystic fibrosis; high density; immunonutrition; intestine; lipoproteins; sphingolipids; sphingosine-1-phosphate |
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Band: | 12 |
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Ausgabe: | 3 |
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Aufsatznummer: | 765 |
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Seitenanzahl: | 11 |
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Fördernde Institution: | Austrian Science Fund FWF grantAustrian Science Fund (FWF) [KLI 284]; Austrian Science Fund (FWF)Austrian Science Fund (FWF) |
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Organisationseinheiten: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
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DDC-Klassifikation: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
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Peer Review: | Referiert |
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Publikationsweg: | Open Access / Gold Open-Access |
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| DOAJ gelistet |
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Lizenz (Deutsch): | CC-BY - Namensnennung 4.0 International |
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