Distinct heat shock factors and chromatin modifications mediate the organ-autonomous transcriptional memory of heat stress
- Plants can be primed by a stress cue to mount a faster or stronger activation of defense mechanisms upon subsequent stress. A crucial component of such stress priming is the modified reactivation of genes upon recurring stress; however, the underlying mechanisms of this are poorly understood. Here, we report that dozens of Arabidopsis thaliana genes display transcriptional memory, i.e. stronger upregulation after a recurring heat stress, that lasts for at least 3 days. We define a set of transcription factors involved in this memory response and show that the transcriptional memory results in enhanced transcriptional activation within minutes of the onset of a heat stress cue. Further, we show that the transcriptional memory is active in all tissues. It may last for up to a week, and is associated during this time with histone H3 lysine 4 hypermethylation. This transcriptional memory is cis-encoded, as we identify a promoter fragment that confers memory onto a heterologous gene. In summary, heat-induced transcriptional memory is aPlants can be primed by a stress cue to mount a faster or stronger activation of defense mechanisms upon subsequent stress. A crucial component of such stress priming is the modified reactivation of genes upon recurring stress; however, the underlying mechanisms of this are poorly understood. Here, we report that dozens of Arabidopsis thaliana genes display transcriptional memory, i.e. stronger upregulation after a recurring heat stress, that lasts for at least 3 days. We define a set of transcription factors involved in this memory response and show that the transcriptional memory results in enhanced transcriptional activation within minutes of the onset of a heat stress cue. Further, we show that the transcriptional memory is active in all tissues. It may last for up to a week, and is associated during this time with histone H3 lysine 4 hypermethylation. This transcriptional memory is cis-encoded, as we identify a promoter fragment that confers memory onto a heterologous gene. In summary, heat-induced transcriptional memory is a widespread and sustained response, and our study provides a framework for future mechanistic studies of somatic stress memory in higher plants.…
Author details: | Hsiang-chin LiuORCiD, Jörn LämkeORCiDGND, Siou-ying Lin, Meng-Ju Hung, Kuan-Ming Liu, Yee-yung CharngORCiD, Isabel BäurleORCiDGND |
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DOI: | https://doi.org/10.1111/tpj.13958 |
ISSN: | 0960-7412 |
ISSN: | 1365-313X |
Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/29752744 |
Title of parent work (English): | The plant journal |
Publisher: | Wiley |
Place of publishing: | Hoboken |
Publication type: | Article |
Language: | English |
Date of first publication: | 2018/05/11 |
Publication year: | 2018 |
Release date: | 2021/10/27 |
Tag: | Arabidopsis thaliana; H3K4 methylation; HSF; epigenetics; heat stress; priming; transcriptional memory |
Volume: | 95 |
Issue: | 3 |
Number of pages: | 13 |
First page: | 401 |
Last Page: | 413 |
Funding institution: | Academia Sinica Postdoctoral Research Fellowship; Ministry of Science and Technology, TaiwanMinistry of Science and Technology, Taiwan [103-2311-B-001-011-MY3]; Academia SinicaAcademia Sinica - Taiwan; Sofja-Kovalevskaja-Award (Alexander-von-Humboldt-Foundation); Deutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [CRC 973]; European Molecular Biology Organization Young Investigator ProgramEuropean Molecular Biology Organization (EMBO) |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
DDC classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Peer review: | Referiert |
Publishing method: | Open Access / Bronze Open-Access |