- Biofilms are complex, sessile microbial communities that are problematic in clinical settings due to their association with survival and pathogenicity of bacteria. The biofilm formation supporting conditions for zoonotic serovars of Salmonella and avian pathogenic E. coli (APEC) from poultry have not been well studied yet. Clinical isolates of zoonotic Salmonella and APEC from poultry were evaluated for biofilm formation in four media at 37 degrees C and 40 degrees C after incubation of 48 and 72 hrs. The biofilms formed in 96 well plates were visualized and quantified with a new module of Aklides system using fluorescence microscope coupled with automated VideoScan Technology. After 72 hrs, brain heart infusion at 40 degrees C and Rappaport-Vassiliadis Soya broth at 37 degrees C were found most suitable for APEC and Salmonella biofilm formations respectively. The new information will be useful for further biofilm associated studies particularly for evaluation of antibiofilm compounds and contribute in infection control. (C) 2020 PVJ.Biofilms are complex, sessile microbial communities that are problematic in clinical settings due to their association with survival and pathogenicity of bacteria. The biofilm formation supporting conditions for zoonotic serovars of Salmonella and avian pathogenic E. coli (APEC) from poultry have not been well studied yet. Clinical isolates of zoonotic Salmonella and APEC from poultry were evaluated for biofilm formation in four media at 37 degrees C and 40 degrees C after incubation of 48 and 72 hrs. The biofilms formed in 96 well plates were visualized and quantified with a new module of Aklides system using fluorescence microscope coupled with automated VideoScan Technology. After 72 hrs, brain heart infusion at 40 degrees C and Rappaport-Vassiliadis Soya broth at 37 degrees C were found most suitable for APEC and Salmonella biofilm formations respectively. The new information will be useful for further biofilm associated studies particularly for evaluation of antibiofilm compounds and contribute in infection control. (C) 2020 PVJ. All rights reserved…
Metadaten| Author details: | Shiza Nawaz, Muhammad Moman KhanORCiD, Jonas Noack, Asad Bashir AwanORCiD, Juliane SchiebelGND, Dirk RoggenbuckORCiD, Peter SchierackORCiD, Yasra Sarwar, Aamir Ali |
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| DOI: | https://doi.org/10.29261/pakvetj/2020.066 |
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| ISSN: | 0253-8318 |
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| ISSN: | 2074-7764 |
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| Title of parent work (English): | Pakistan veterinary journal |
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| Publisher: | University of Agriculture, Faculty of Veterinary Science |
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| Place of publishing: | Faisalabad |
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| Publication type: | Article |
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| Language: | English |
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| Date of first publication: | 2020/07/25 |
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| Publication year: | 2020 |
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| Release date: | 2024/01/17 |
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| Tag: | APEC; Salmonella; biofilm formation; video scan technology |
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| Volume: | 40 |
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| Issue: | 4 |
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| Number of pages: | 4 |
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| First page: | 527 |
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| Last Page: | 530 |
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| Funding institution: | project 'FISH ftir Tumor and Erregerdiagnostik mittels VideoScan -; Federal Ministry of Education and Research (BMBF, Germany)Federal; Ministry of Education & Research (BMBF) [03PSZZFIA] |
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| Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
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| DDC classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
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| Peer review: | Referiert |
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| Publishing method: | Open Access / Gold Open-Access |
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| DOAJ gelistet |
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| License (German): |  CC-BY-NC - Namensnennung, nicht kommerziell 4.0 International |
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