Transcription factor RD26 is a key regulator of metabolic reprogramming during dark-induced senescence
- Leaf senescence is a key process in plants that culminates in the degradation of cellular constituents and massive reprogramming of metabolism for the recovery of nutrients from aged leaves for their reuse in newly developing sinks. We used molecular-biological and metabolomics approaches to identify NAC transcription factor (TF) RD26 as an important regulator of metabolic reprogramming in Arabidopsis thaliana. RD26 directly activates CHLOROPLAST VESICULATION (CV), encoding a protein crucial for chloroplast protein degradation, concomitant with an enhanced protein loss in RD26 over-expressors during senescence, but a reduced decline of protein in rd26 knockout mutants. RD26 also directly activates LKR/SDH involved in lysine catabolism, and PES1 important for phytol degradation. Metabolic profiling revealed reduced c-aminobutyric acid (GABA) in RD26 overexpressors, accompanied by the induction of respective catabolic genes. Degradation of lysine, phytol and GABA is instrumental for maintaining mitochondrial respiration inLeaf senescence is a key process in plants that culminates in the degradation of cellular constituents and massive reprogramming of metabolism for the recovery of nutrients from aged leaves for their reuse in newly developing sinks. We used molecular-biological and metabolomics approaches to identify NAC transcription factor (TF) RD26 as an important regulator of metabolic reprogramming in Arabidopsis thaliana. RD26 directly activates CHLOROPLAST VESICULATION (CV), encoding a protein crucial for chloroplast protein degradation, concomitant with an enhanced protein loss in RD26 over-expressors during senescence, but a reduced decline of protein in rd26 knockout mutants. RD26 also directly activates LKR/SDH involved in lysine catabolism, and PES1 important for phytol degradation. Metabolic profiling revealed reduced c-aminobutyric acid (GABA) in RD26 overexpressors, accompanied by the induction of respective catabolic genes. Degradation of lysine, phytol and GABA is instrumental for maintaining mitochondrial respiration in carbon-limiting conditions during senescence. RD26 also supports the degradation of starch and the accumulation of mono-and disaccharides during senescence by directly enhancing the expression of AMY1, SFP1 and SWEET15 involved in carbohydrate metabolism and transport. Collectively, during senescence RD26 acts by controlling the expression of genes across the entire spectrum of the cellular degradation hierarchy.…
Author details: | Iman KamranfarORCiDGND, Gang-Ping Xue, Takayuki TohgeORCiD, Mastoureh SedaghatmehrORCiDGND, Alisdair FernieORCiDGND, Salma BalazadehORCiDGND, Bernd Mueller-RoeberORCiDGND |
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DOI: | https://doi.org/10.1111/nph.15127 |
ISSN: | 0028-646X |
ISSN: | 1469-8137 |
Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/29659022 |
Title of parent work (English): | New phytologist : international journal of plant science |
Publisher: | Wiley |
Place of publishing: | Hoboken |
Publication type: | Article |
Language: | English |
Date of first publication: | 2018/04/16 |
Publication year: | 2018 |
Release date: | 2021/11/24 |
Tag: | Arabidopsis; fatty acid; primary metabolism; protein and amino acid degradation; respiration; senescence |
Volume: | 218 |
Issue: | 4 |
Number of pages: | 15 |
First page: | 1543 |
Last Page: | 1557 |
Funding institution: | University of Potsdam; Max Planck Institute of Molecular Plant Physiology; Iran Ministry of Science; Chamran University of Ahvaz; Deutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [FOR 948, MU 1199/14-2, BA4769/1-2, MU 1199/16-1] |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
DDC classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Peer review: | Referiert |
Publishing method: | Open Access / Hybrid Open-Access |
License (German): | ![]() |