CytoSeg 2.0
- Motivation: Actin filaments (AFs) are dynamic structures that substantially change their organization over time. The dynamic behavior and the relatively low signal-to-noise ratio during live-cell imaging have rendered the quantification of the actin organization a difficult task. Results: We developed an automated image-based framework that extracts AFs from fluorescence microscopy images and represents them as networks, which are automatically analyzed to identify and compare biologically relevant features. Although the source code is freely available, we have now implemented the framework into a graphical user interface that can be installed as a Fiji plugin, thus enabling easy access by the research community.
| Author details: | Jacqueline NowakORCiDGND, Kristin Gennermann, Staffan PerssonORCiDGND, Zoran NikoloskiORCiDGND |
|---|---|
| DOI: | https://doi.org/10.1093/bioinformatics/btaa035 |
| ISSN: | 1367-4803 |
| ISSN: | 1460-2059 |
| Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/31971582 |
| Title of parent work (English): | Bioinformatics |
| Subtitle (English): | automated extraction of actin filaments |
| Publisher: | Oxford Univ. Press |
| Place of publishing: | Oxford |
| Publication type: | Article |
| Language: | English |
| Date of first publication: | 2020/01/23 |
| Publication year: | 2020 |
| Release date: | 2023/07/13 |
| Volume: | 36 |
| Issue: | 9 |
| Number of pages: | 2 |
| First page: | 2950 |
| Last Page: | 2951 |
| Funding institution: | German Federal Ministry of Research and Education project SHAPENET; [031L0177A]; Australian Research CouncilAustralian Research Council; [DP19001941, FT160100218]; IRRTF grant from the University of Melbourne |
| Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
| DDC classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
| Peer review: | Referiert |
| Publishing method: | Open Access / Hybrid Open-Access |
| DOAJ gelistet | |
| License (German): |  CC-BY - Namensnennung 4.0 International |
