Development of a molecularly imprinted polymer-based electrochemical sensor for tyrosinase
- For the first time a molecularly imprinted polymer (MIP)-based sensor for tyrosinase is described. This sensor is based on the electropolymerization of scopoletin or o-phenylenediamine in the presence of tyrosinase from mushrooms, which has a high homology to the human enzyme. The template was removed either by treatment with proteinase Kor by alkaline treatment. The measuring signal was generated either by measuring the formation of a product by the target enzyme or by evaluation of the permeability of the redox marker ferricyanide. The o-phenylenediamine-based MIP sensor has a linear measuring range up to 50 nM of tyrosinase with a limit of detection of 3.97 nM (R 2 = 0.994) and shows good discrimination towards other proteins, e.g., bovine serum albumin and cytochrome c.
Verfasserangaben: | Aysu YarmanORCiDGND |
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DOI: | https://doi.org/10.3906/kim-1708-68 |
ISSN: | 1300-0527 |
Titel des übergeordneten Werks (Englisch): | Turkish journal of chemistry |
Verlag: | Türkiye Bilimsel ve Teknik Araştırma Kurumu |
Verlagsort: | Ankara |
Publikationstyp: | Wissenschaftlicher Artikel |
Sprache: | Englisch |
Datum der Erstveröffentlichung: | 06.11.2017 |
Erscheinungsjahr: | 2017 |
Datum der Freischaltung: | 31.03.2022 |
Freies Schlagwort / Tag: | Molecularly imprinted polymers; biomimetic sensors; electropolymerization; ophenylenediamine; scopoletin; tyrosinase |
Band: | 42 |
Ausgabe: | 2 |
Seitenanzahl: | 9 |
Erste Seite: | 346 |
Letzte Seite: | 354 |
Fördernde Institution: | Deutsche Forschungsgemeinschaft (DFG)German Research Foundation (DFG) [EXC 314]; Turkish-German University Scientific Research Projects Commission [2016BF0011] |
Organisationseinheiten: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
DDC-Klassifikation: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Peer Review: | Referiert |
Publikationsweg: | Open Access / Bronze Open-Access |