Sarah R. Smith, Chris L. Dupont, James K. McCarthy, Jared T. Broddrick, Miroslav Obornik, Ales Horak, Zoltán Füssy, Jaromir Cihlar, Sabrina Kleessen, Hong Zheng, John P. McCrow, Kim K. Hixson, Wagner L. Araujo, Adriano Nunes-Nesi, Alisdair Fernie, Zoran Nikoloski, Bernhard O. Palsson, Andrew E. Allen
- Diatoms outcompete other phytoplankton for nitrate, yet little is known about the mechanisms underpinning this ability. Genomes and genome-enabled studies have shown that diatoms possess unique features of nitrogen metabolism however, the implications for nutrient utilization and growth are poorly understood. Using a combination of transcriptomics, proteomics, metabolomics, fluxomics, and flux balance analysis to examine short-term shifts in nitrogen utilization in the model pennate diatom in Phaeodactylum tricornutum, we obtained a systems-level understanding of assimilation and intracellular distribution of nitrogen. Chloroplasts and mitochondria are energetically integrated at the critical intersection of carbon and nitrogen metabolism in diatoms. Pathways involved in this integration are organelle-localized GS-GOGAT cycles, aspartate and alanine systems for amino moiety exchange, and a split-organelle arginine biosynthesis pathway that clarifies the role of the diatom urea cycle. This unique configuration allows diatoms toDiatoms outcompete other phytoplankton for nitrate, yet little is known about the mechanisms underpinning this ability. Genomes and genome-enabled studies have shown that diatoms possess unique features of nitrogen metabolism however, the implications for nutrient utilization and growth are poorly understood. Using a combination of transcriptomics, proteomics, metabolomics, fluxomics, and flux balance analysis to examine short-term shifts in nitrogen utilization in the model pennate diatom in Phaeodactylum tricornutum, we obtained a systems-level understanding of assimilation and intracellular distribution of nitrogen. Chloroplasts and mitochondria are energetically integrated at the critical intersection of carbon and nitrogen metabolism in diatoms. Pathways involved in this integration are organelle-localized GS-GOGAT cycles, aspartate and alanine systems for amino moiety exchange, and a split-organelle arginine biosynthesis pathway that clarifies the role of the diatom urea cycle. This unique configuration allows diatoms to efficiently adjust to changing nitrogen status, conferring an ecological advantage over other phytoplankton taxa.…
MetadatenAuthor details: | Sarah R. Smith, Chris L. Dupont, James K. McCarthyORCiD, Jared T. Broddrick, Miroslav Obornik, Ales Horak, Zoltán FüssyORCiD, Jaromir Cihlar, Sabrina Kleessen, Hong Zheng, John P. McCrow, Kim K. HixsonORCiD, Wagner L. AraujoORCiD, Adriano Nunes-Nesi, Alisdair FernieORCiDGND, Zoran NikoloskiORCiDGND, Bernhard O. PalssonORCiD, Andrew E. AllenORCiD |
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DOI: | https://doi.org/10.1038/s41467-019-12407-y |
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ISSN: | 2041-1723 |
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Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/31591397 |
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Title of parent work (English): | Nature Communications |
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Publisher: | Nature Publ. Group |
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Place of publishing: | London |
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Publication type: | Article |
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Language: | English |
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Date of first publication: | 2019/10/07 |
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Publication year: | 2019 |
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Release date: | 2020/12/07 |
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Tag: | Biochemistry; Computational biology and bioinformatics; Evolution; Microbiology; Molecular biology |
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Volume: | 10 |
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Number of pages: | 14 |
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Funding institution: | National Science FoundationNational Science Foundation (NSF) [NSF-MCB-1024913, NSF-MCB-1818390]; United States Department of Energy Genomics Science Program [DE-SC00006719, DE-SC0008593, DE-SC0008701]; Gordon and Betty Moore FoundationGordon and Betty Moore Foundation [GBMF3828]; Czech Science FoundationGrant Agency of the Czech Republic [15-17643S]; ERDF/ESF [CZ.02.1.01/0.0/0.0/16_019/0000759]; Department of Department of Energy (DOE) |
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Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
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DDC classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
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Peer review: | Referiert |
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Publishing method: | Open Access / Gold Open-Access |
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| DOAJ gelistet |
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