Differentially-charged liposomes interact with alphaherpesviruses and interfere with virus entry
- Exposure of phosphatidylserine (PS) in the outer leaflet of the plasma membrane is induced by infection with several members of the Alphaherpesvirinae subfamily. There is evidence that PS is used by the equine herpesvirus type 1 (EHV-1) during entry, but the exact role of PS and other phospholipids in the entry process remains unknown. Here, we investigated the interaction of differently charged phospholipids with virus particles and determined their influence on infection. Our data show that liposomes containing negatively charged PS or positively charged DOTAP (N-[1-(2,3-Dioleoyloxy)propyl]-N,N,N-trimethylammonium) inhibited EHV-1 infection, while neutral phosphatidylcholine (PC) had no effect. Inhibition of infection with PS was transient, decreased with time, and was dose dependent. Our findings indicate that both cationic and anionic phospholipids can interact with the virus and reduce infectivity, while, presumably, acting through different mechanisms. Charged phospholipids were found to have antiviral effects and may be used toExposure of phosphatidylserine (PS) in the outer leaflet of the plasma membrane is induced by infection with several members of the Alphaherpesvirinae subfamily. There is evidence that PS is used by the equine herpesvirus type 1 (EHV-1) during entry, but the exact role of PS and other phospholipids in the entry process remains unknown. Here, we investigated the interaction of differently charged phospholipids with virus particles and determined their influence on infection. Our data show that liposomes containing negatively charged PS or positively charged DOTAP (N-[1-(2,3-Dioleoyloxy)propyl]-N,N,N-trimethylammonium) inhibited EHV-1 infection, while neutral phosphatidylcholine (PC) had no effect. Inhibition of infection with PS was transient, decreased with time, and was dose dependent. Our findings indicate that both cationic and anionic phospholipids can interact with the virus and reduce infectivity, while, presumably, acting through different mechanisms. Charged phospholipids were found to have antiviral effects and may be used to inhibit EHV-1 infection.…
| Verfasserangaben: | Oleksandr KolyvushkoORCiDGND, Juliane Latzke, Ismail DahmaniORCiDGND, Nikolaus OsterriederORCiDGND, Salvatore ChiantiaORCiDGND, Walid AzabORCiD |
|---|---|
| DOI: | https://doi.org/10.3390/pathogens9050359 |
| ISSN: | 2076-0817 |
| Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/32397270 |
| Titel des übergeordneten Werks (Englisch): | Pathogens |
| Verlag: | MDPI |
| Verlagsort: | Basel |
| Publikationstyp: | Wissenschaftlicher Artikel |
| Sprache: | Englisch |
| Datum der Erstveröffentlichung: | 08.05.2020 |
| Erscheinungsjahr: | 2020 |
| Datum der Freischaltung: | 23.03.2023 |
| Freies Schlagwort / Tag: | EHV-1; alphaherpesvirus; inhibition; interaction; pathogen host; phosphatidylserine |
| Band: | 9 |
| Ausgabe: | 5 |
| Aufsatznummer: | 359 |
| Seitenanzahl: | 9 |
| Fördernde Institution: | Deutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [DFG AZ; 97/3-2]; Morris Animal Foundation [D19EQ-003]; Freie Universitat Berlin |
| Organisationseinheiten: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
| DDC-Klassifikation: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
| 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit | |
| Peer Review: | Referiert |
| Publikationsweg: | Open Access / Gold Open-Access |
| DOAJ gelistet | |
| Lizenz (Deutsch): |  CC-BY - Namensnennung 4.0 International |
| Externe Anmerkung: | Zweitveröffentlichung in der Schriftenreihe Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe ; 1088 |
