Reversible and rapid transfer-RNA deactivation as a mechanism of translational repression in stress
- Stress-induced changes of gene expression are crucial for survival of eukaryotic cells. Regulation at the level of translation provides the necessary plasticity for immediate changes of cellular activities and protein levels. In this study, we demonstrate that exposure to oxidative stress results in a quick repression of translation by deactivation of the aminoacylends of all transfer-RNA (tRNA). An oxidative-stress activated nuclease, angiogenin, cleaves first within the conserved single-stranded 3'-CCA termini of all tRNAs, thereby blocking their use in translation. This CCA deactivation is reversible and quickly repairable by the CCA-adding enzyme [ATP(CTP): tRNA nucleotidyltransferase]. Through this mechanism the eukaryotic cell dynamically represses and reactivates translation at low metabolic costs.
Author details: | Andreas Czech, Sandra Wende, Mario Moerl, Tao Pan, Zoya Ignatova |
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DOI: | https://doi.org/10.1371/journal.pgen.1003767 |
ISSN: | 1553-7404 |
Title of parent work (English): | PLoS Genetics : a peer-reviewed, open-access journal |
Publisher: | PLoS |
Place of publishing: | San Fransisco |
Publication type: | Article |
Language: | English |
Year of first publication: | 2013 |
Publication year: | 2013 |
Release date: | 2017/03/26 |
Volume: | 9 |
Issue: | 8 |
Number of pages: | 9 |
Funding institution: | Stiftung des Deutschen Volkes; DFG |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
Peer review: | Referiert |
Publishing method: | Open Access |