TY  - JOUR
A1  - Czech, Andreas
A1  - Wende, Sandra
A1  - Moerl, Mario
A1  - Pan, Tao
A1  - Ignatova, Zoya
T1  - Reversible and rapid transfer-RNA deactivation as a mechanism of translational repression in stress
T2  - PLoS Genetics : a peer-reviewed, open-access journal
N2  - Stress-induced changes of gene expression are crucial for survival of eukaryotic cells. Regulation at the level of translation provides the necessary plasticity for immediate changes of cellular activities and protein levels. In this study, we demonstrate that exposure to oxidative stress results in a quick repression of translation by deactivation of the aminoacylends of all transfer-RNA (tRNA). An oxidative-stress activated nuclease, angiogenin, cleaves first within the conserved single-stranded 3'-CCA termini of all tRNAs, thereby blocking their use in translation. This CCA deactivation is reversible and quickly repairable by the CCA-adding enzyme [ATP(CTP): tRNA nucleotidyltransferase]. Through this mechanism the eukaryotic cell dynamically represses and reactivates translation at low metabolic costs.
Y1  - 2013
UR  - https://publishup.uni-potsdam.de/frontdoor/index/index/docId/34830
SN  - 1553-7404
VL  - 9
IS  - 8
PB  - PLoS
CY  - San Fransisco
ER  -