Refine
Year of publication
- 2013 (1317) (remove)
Document Type
- Article (912)
- Doctoral Thesis (174)
- Postprint (60)
- Preprint (48)
- Conference Proceeding (42)
- Review (36)
- Monograph/Edited Volume (30)
- Other (7)
- Habilitation Thesis (2)
- Master's Thesis (2)
Language
- English (1317) (remove)
Keywords
- Curriculum Framework (17)
- European values education (17)
- Europäische Werteerziehung (17)
- Familie (17)
- Family (17)
- Lehrevaluation (17)
- Studierendenaustausch (17)
- Unterrichtseinheiten (17)
- curriculum framework (17)
- lesson evaluation (17)
Institute
- Institut für Biochemie und Biologie (254)
- Institut für Physik und Astronomie (186)
- Institut für Geowissenschaften (181)
- Institut für Chemie (141)
- Department Psychologie (71)
- Institut für Mathematik (63)
- Wirtschaftswissenschaften (41)
- Department Linguistik (37)
- Institut für Informatik und Computational Science (35)
- Institut für Umweltwissenschaften und Geographie (34)
Ten ice-sheet models are used to study sensitivity of the Greenland and Antarctic ice sheets to prescribed changes of surface mass balance, sub-ice-shelf melting and basal sliding. Results exhibit a large range in projected contributions to sea-level change. In most cases, the ice volume above flotation lost is linearly dependent on the strength of the forcing. Combinations of forcings can be closely approximated by linearly summing the contributions from single forcing experiments, suggesting that nonlinear feedbacks are modest. Our models indicate that Greenland is more sensitive than Antarctica to likely atmospheric changes in temperature and precipitation, while Antarctica is more sensitive to increased ice-shelf basal melting. An experiment approximating the Intergovernmental Panel on Climate Change's RCP8.5 scenario produces additional first-century contributions to sea level of 22.3 and 8.1 cm from Greenland and Antarctica, respectively, with a range among models of 62 and 14 cm, respectively. By 200 years, projections increase to 53.2 and 26.7 cm, respectively, with ranges of 79 and 43 cm. Linear interpolation of the sensitivity results closely approximates these projections, revealing the relative contributions of the individual forcings on the combined volume change and suggesting that total ice-sheet response to complicated forcings over 200 years can be linearized.
Deep into the second half of the twentieth century the traditionalist definition of India as a country of villages remained dominant in official political rhetoric as well as cultural production. In the past two decades or so, this ruralist paradigm has been effectively superseded by a metropolitan imaginary in which the modern, globalised megacity increasingly functions as representative of India as a whole. Has the village, then, entirely vanished from the cultural imaginary in contemporary India? Addressing economic practices from upper-class consumerism to working-class family support strategies, this paper attempts to trace how 'the village' resurfaces or survives as a cultural reference point in the midst of the urban.
Effects of intraspecific and community density on the lifetime fecundity of long-lived shrubs
(2013)
Intra- and interspecific density dependence has profound consequences for plant population and community dynamics. In long-lived plants, however, lifetime patterns and mechanisms of density dependence are difficult to study. Here, we examine effects of intraspecific and community density on the lifetime fecundity of two long-lived shrub species from South African Fynbos: Protea repens (animal-pollinated, hermaphroditic) and Leucadendron rubrum (wind-pollinated, dioecious). Both species are serotinous, retaining seeds in cones until fire kills the mother plant. We measured lifetime fecundity as the product of cone number, proportion of cones that are not damaged by predation and seed set (fertile seeds per intact cone). Intraspecific and community densities were quantified by counting individuals of target species and all Proteaceae in small- and large-scale neighbourhoods (10 m and 50 m radius) around each focal individual. Additionally, we determined the age and size of focal individuals. We found that lifetime fecundity of the wind-pollinated L rubrum is density independent. In contrast, the lifetime fecundity of the animal-pollinated P. repens increases with large-scale intraspecific density and shows a hump-shaped relationship to large-scale community density. Community density has a hump-shaped effect on seed set (probably through partial absence of generalized pollinators at low and competition for pollinators at high densities) and negatively affects cone number per individual. For both species, plant age decreases seed set while increasing lifetime fecundity. The qualitative differences in the density dependence of lifetime fecundity may arise from differences between animal and wind pollination. In particular, interactions with generalized animal pollinators may cause community-level Allee effects with profound consequences for the future dynamics of long-lived plant populations and communities.
Calcium phosphate nanofibers with a diameter of only a few nanometers and a cotton-ball-like aggregate morphology have been reported several times in the literature. Although fiber formation seems reproducible in a variety of conditions, the crystal structure and chemical composition of the fibers have been elusive. Using scanning transmission electron microscopy, low dose electron (nano) diffraction, energy-dispersive X-ray spectroscopy, and energy-filtered transmission electron microscopy, we have assigned crystal structures and chemical compositions to the fibers. Moreover, we demonstrate that the mineralization process yields true polymer/calcium phosphate hybrid materials where the block copolymer template is closely associated with the calcium phosphate.
A novel procedure has been developed to conduct cell structure measurements on increment core samples of conifers. The procedure combines readily available hardware and software equipment. The essential part of the procedure is the application of a confocal laser scanning microscope (CLSM) which captures images directly from increment cores surfaced with the advanced WSL core-microtome. Cell wall and lumen are displayed with a strong contrast due to the monochrome black and green nature of the images. Consecutive images are merged into long images representing entire increment cores which are then analysed for cell structures in suitable software.
A problem encountered by many distributed hydrological modelling studies is high simulation errors at interior gauges when the model is only globally calibrated at the outlet. We simulated river runoff in the Elbe River basin in central Europe (148 268 km(2)) with the semi-distributed eco-hydrological model SWIM (Soil and Water Integrated Model). While global parameter optimisation led to Nash-Sutcliffe efficiencies of 0.9 at the main outlet gauge, comparisons with measured runoff series at interior points revealed large deviations. Therefore, we compared three different strategies for deriving sub-basin evapotranspiration: (1) modelled by SWIM without any spatial calibration, (2) derived from remotely sensed surface temperatures, and (3) calculated from long-term precipitation and discharge data. The results show certain consistencies between the modelled and the remote sensing based evapotranspiration rates, but there seems to be no correlation between remote sensing and water balance based estimations. Subsequent analyses for single sub-basins identify amongst others input weather data and systematic error amplification in inter-gauge discharge calculations as sources of uncertainty. The results encourage careful utilisation of different data sources for enhancements in distributed hydrological modelling.
The relevance of biological Si cycling for dissolved silica (DSi) export from terrestrial biogeosystems is still in debate. Even in systems showing a high content of weatherable minerals, like Cambisols on volcanic tuff, biogenic Si (BSi) might contribute > 50% to DSi (Gerard et al., 2008). However, the number of biogeosystem studies is rather limited for generalized conclusions. To cover one end of controlling factors on DSi, i.e., weatherable minerals content, we studied a forested site with absolute quartz dominance (> 95 %). Here we hypothesise minimal effects of chemical weathering of silicates on DSi. During a four year observation period (05/2007-04/2011), we quantified (i) internal and external Si fluxes of a temperate-humid biogeosystem (beech, 120 yr) by BIOME-BGC (version ZALF), (ii) related Si budgets, and (iii) Si pools in soil and beech, chemically as well as by SEM-EDX. For the first time two compartments of biogenic Si in soils were analysed, i.e., phytogenic and zoogenic Si pool (testate amoebae). We quantified an average Si plant uptake of 35 kg Si ha(-1) yr(-1) - most of which is recycled to the soil by litterfall - and calculated an annual biosilicification from idiosomic testate amoebae of 17 kg Si ha(-1). The comparatively high DSi concentrations (6 mg L-1) and DSi exports (12 kg Si ha(-1) yr(-1)) could not be explained by chemical weathering of feldspars or quartz dissolution. Instead, dissolution of a relictic, phytogenic Si pool seems to be the main process for the DSi observed. We identified canopy closure accompanied by a disappearance of grasses as well as the selective extraction of pine trees 30 yr ago as the most probable control for the phenomena observed. From our results we concluded the biogeosystem to be in a transient state in terms of Si cycling.
Mean age of carbon in fine roots from temperate forests and grasslands with different management
(2013)
Fine roots are the most dynamic portion of a plant's root system and a major source of soil organic matter. By altering plant species diversity and composition, soil conditions and nutrient availability, and consequently belowground allocation and dynamics of root carbon (C) inputs, land-use and management changes may influence organic C storage in terrestrial ecosystems. In three German regions, we measured fine root radiocarbon (C-14) content to estimate the mean time since C in root tissues was fixed from the atmosphere in 54 grassland and forest plots with different management and soil conditions. Although root biomass was on average greater in grasslands 5.1 +/- 0.8 g (mean +/- SE, n = 27) than in forests 3.1 +/- 0.5 g (n = 27) (p < 0.05), the mean age of C in fine roots in forests averaged 11.3 +/- 1.8 yr and was older and more variable compared to grasslands 1.7 +/- 0.4 yr (p < 0.001). We further found that management affects the mean age of fine root C in temperate grasslands mediated by changes in plant species diversity and composition. Fine root mean C age is positively correlated with plant diversity (r = 0.65) and with the number of perennial species (r = 0.77). Fine root mean C age in grasslands was also affected by study region with averages of 0.7 +/- 0.1 yr (n= 9) on mostly organic soils in northern Germany and of 1.8 +/- 0.3 yr (n = 9) and 2.6 +/- 0.3 (n = 9) in central and southern Germany (p < 0.05). This was probably due to differences in soil nutrient contents and soil moisture conditions between study regions, which affected plant species diversity and the presence of perennial species. Our results indicate more long-lived roots or internal redistribution of C in perennial species and suggest linkages between fine root C age and management in grasslands. These findings improve our ability to predict and model belowground C fluxes across broader spatial scales.
Predictions of marine ice-sheet behaviour require models able to simulate grounding-line migration. We present results of an intercomparison experiment for plan-view marine ice-sheet models. Verification is effected by comparison with approximate analytical solutions for flux across the grounding line using simplified geometrical configurations (no lateral variations, no buttressing effects from lateral drag). Perturbation experiments specifying spatial variation in basal sliding parameters permitted the evolution of curved grounding lines, generating buttressing effects. The experiments showed regions of compression and extensional flow across the grounding line, thereby invalidating the boundary layer theory. Steady-state grounding-line positions were found to be dependent on the level of physical model approximation. Resolving grounding lines requires inclusion of membrane stresses, a sufficiently small grid size (<500 m), or subgrid interpolation of the grounding line. The latter still requires nominal grid sizes of <5 km. For larger grid spacings, appropriate parameterizations for ice flux may be imposed at the grounding line, but the short-time transient behaviour is then incorrect and different from models that do not incorporate grounding-line parameterizations. The numerical error associated with predicting grounding-line motion can be reduced significantly below the errors associated with parameter ignorance and uncertainties in future scenarios.
Among the climatological events of the last millennium, the Northern Hemisphere Medieval Climate Anomaly succeeded by the Little Ice Age are of exceptional importance. The origin of these regional climate anomalies remains a subject of debate and besides external influences like solar and volcanic activity, internal dynamics of the climate system might have also played a dominant role. Here, we present transient last millennium simulations of the fully coupled model of intermediate complexity Climber 3a forced with stochastically reconstructed wind-stress fields. Our results indicate that short-lived volcanic eruptions might have triggered a cascade of sea ice ocean feedbacks in the North Atlantic, ultimately leading to a persistent regime shift in the ocean circulation. We find that an increase in the Nordic Sea sea-ice extent on decadal timescales as a consequence of major volcanic eruptions in our model leads to a spin-up of the subpolar gyre and a weakened Atlantic meridional overturning circulation, eventually causing a persistent, basin-wide cooling. These results highlight the importance of regional climate feedbacks such as a regime shift in the subpolar gyre circulation for understanding the dynamics of past and future climate.
Huntington disease (HD), a dominantly inherited neurodegenerative disorder caused by the expansion of a CAG-encoded polyglutamine (polyQ) repeat in huntingtin (Htt), displays a highly heterogeneous etiopathology and disease onset. Here, we show that the translation of expanded CAG repeats in mutant Htt exon 1 leads to a depletion of charged glutaminyl-transfer RNA (tRNA) Gln-CUG that pairs exclusively to the CAG codon. This results in translational frameshifting and the generation of various transframe-encoded species that differently modulate the conformational switch to nucleate fibrillization of the parental polyQ protein. Intriguingly, the frameshifting frequency varies strongly among different cell lines and is higher in cells with intrinsically lower concentrations of tRNA Gln-CUG. The concentration of tRNA Gln-CUG also differs among different brain areas in the mouse. We propose that translational frameshifting may act as a significant disease modifier that contributes to the cell-selective neurotoxicity and disease course heterogeneity of HD on both cellular and individual levels.
Habitat fragmentation is one of the most important causes for the decline of plant species. However, plants differing in phylogeny, habitat requirements and biology are likely to respond differently to habitat fragmentation. We ask whether case studies on the effects of habitat fragmentation conducted so far allow generalizations about its effects on the fitness and genetic diversity of populations of endangered plant species. We compared the characteristics of plant species endangered in Germany whose sensitivity to habitat fragmentation had been studied with those of the endangered species that had not been studied. We found strong discrepancies between the two groups with regard to their taxonomy and traits relevant to their sensitivity to habitat fragmentation. Monocots, graminoids, clonal, abiotically pollinated and self compatible species were underrepresented among the studied species, and most study species were from a few habitat types, in particular grasslands. We conclude that our current knowledge of the effects of habitat fragmentation on plant populations is not sufficient to provide widely applicable guidelines for species management. The selection of species studied so far has been biased toward species from certain habitats and species exhibiting traits that probably make them vulnerable to habitat fragmentation. Future studies should include community-wide approaches in different habitats, e.g. re-visitation studies in which the species pool is assessed at different time intervals, and population-biological studies of species from a wide range of habitats, and of different life forms and growth strategies. A more representative picture of the effects of habitat fragmentation would allow a better assessment of threats and more specific recommendations for optimally managing populations of endangered plants.
The Tuz Golu Basin is the largest sedimentary depression located at the center of the Central Anatolian Plateau, an extensive, low-relief region with elevations of ca. 1 km located between the Pontide and Tauride mountains. Presently, the basin morphology and sedimentation processes are mainly controlled by the extensional Tuz Golu Fault Zone in the east and the transtensional Inonu-Eskisehir Fault System in the west. The purpose of this study is to contribute to the understanding of the Plio-Quaternary deformation history and to refine the timing of the latest extensional phase of the Tuz Golu Basin. Field observations, kinematic analyses, interpretations of seismic reflection lines, and Ar-40/Ar-39 dating of a key ignimbrite layer suggest that a regional phase of NNW-SSE to NE-SW contraction ended by 6.81 +/- 0.24 Ma and was followed by N-S to NE-SW extension during the Pliocene-Quaternary periods. Based on sedimentological and chronostratigraphic markers, the average vertical displacement rates over the past 5 or 3 Ma with respect to the central part of Tuz Golu Lake are 0.03 to 0.05 mm/year for the fault system at the western flank of the basin and 0.08 to 0.13 mm/year at the eastern flank. Paleo-shorelines of the Tuz Golu Lake, vestiges of higher lake levels related to Quaternary climate change, are important strain markers and were formed during Last Glacial Maximum conditions as indicated by a radiocarbon age of 21.8 +/- 0.4 ka BP obtained from a stromatolitic crust. Geomorphic observations and deformed lacustrine shorelines suggest that the main strand of the Tuz Golu Fault Zone straddling the foothills of the Sereflikochisar-Aksaray range has not been active during the Holocene. Instead, deformation appears to have migrated towards the interior of the basin along an offshore fault that runs immediately west of Sereflikochisar Peninsula. This basinward migration of deformation is probably associated with various processes acting at the lithospheric scale, such as plateau uplift and/or microplate extrusion.
Many applications, such as intermittent data assimilation, lead to a recursive application of Bayesian inference within a Monte Carlo context. Popular data assimilation algorithms include sequential Monte Carlo methods and ensemble Kalman filters (EnKFs). These methods differ in the way Bayesian inference is implemented. Sequential Monte Carlo methods rely on importance sampling combined with a resampling step, while EnKFs utilize a linear transformation of Monte Carlo samples based on the classic Kalman filter. While EnKFs have proven to be quite robust even for small ensemble sizes, they are not consistent since their derivation relies on a linear regression ansatz. In this paper, we propose another transform method, which does not rely on any a priori assumptions on the underlying prior and posterior distributions. The new method is based on solving an optimal transportation problem for discrete random variables.
In this paper a change-point detection method is proposed by extending the singular spectrum transformation (SST) developed as one of the capabilities of singular spectrum analysis (SSA). The method uncovers change points related with trends and periodicities. The potential of the proposed method is demonstrated by analysing simple model time series including linear functions and sine functions as well as real world data (precipitation data in Kenya). A statistical test of the results is proposed based on a Monte Carlo simulation with surrogate methods. As a result, the successful estimation of change points as inherent properties in the representative time series of both trend and harmonics is shown. With regards to the application, we find change points in the precipitation data of Kenyan towns (Nakuru, Naivasha, Narok, and Kisumu) which coincide with the variability of the Indian Ocean Dipole (IOD) suggesting its impact of extreme climate in East Africa.
In this BEEBOOK paper we present a set of established methods for quantifying honey bee behaviour. We start with general methods for preparing bees for behavioural assays. Then we introduce assays for quantifying sensory responsiveness to gustatory, visual and olfactory stimuli. Presentation of more complex behaviours like appetitive and aversive learning under controlled laboratory conditions and learning paradigms under free-flying conditions will allow the reader to investigate a large range of cognitive skills in honey bees. Honey bees are very sensitive to changing temperatures. We therefore present experiments which aim at analysing honey bee locomotion in temperature gradients. The complex flight behaviour of honey bees can be investigated under controlled conditions in the laboratory or with sophisticated technologies like harmonic radar or RFID in the field. These methods will be explained in detail in different sections. Honey bees are model organisms in behavioural biology for their complex yet plastic division of labour. To observe the daily behaviour of individual bees in a colony, classical observation hives are very useful. The setting up and use of typical observation hives will be the focus of another section. The honey bee dance language has important characteristics of a real language and has been the focus of numerous studies. We here discuss the background of the honey bee dance language and describe how it can be studied. Finally, the mating of a honey bee queen with drones is essential to survival of the entire colony. We here give detailed and structured information how the mating behaviour of drones and queens can be observed and experimentally manipulated.
The ultimate goal of this chapter is to provide the reader with a comprehensive set of experimental protocols for detailed studies on all aspects of honey bee behaviour including investigation of pesticide and insecticide effects.
The spatial and temporal variability of a low-centred polygon on the eastern floodplain area of the lower Anabar River (72.070 degrees N, 113.921 degrees E; northern Yakutia, Siberia) has been investigated using a multi-method approach. The present-day vegetation in each square metre was analysed, revealing a community of Larix, shrubby Betula, and Salix on the polygon rim, a dominance of Carex and Andromeda polifolia in the rim-to-pond transition zone, and a predominantly monospecific Scorpidium scorpioides coverage within the pond. The total organic carbon (TOC) content, TOC/TN (total nitrogen) ratio, grain size, vascular plant macrofossils, moss remains, diatoms, and pollen were analysed for two vertical sections and a sediment core from a transect across the polygon. Radiocarbon dating indicates that the formation of the polygon started at least 1500 yr ago; the general positions of the pond and rim have not changed since that time. Two types of pond vegetation were identified, indicating two contrasting development stages of the polygon. The first was a well-established moss association, dominated by submerged or floating Scorpidium scorpioides and/or Drepanocladus spp. and overgrown by epiphytic diatoms such as Tabellaria flocculosa and Eunotia taxa. This stage coincides temporally with a period in which the polygon was only drained by lateral subsurface water flow, as indicated by mixed grain sizes. A different moss association occurred during times of repeated river flooding (indicated by homogeneous medium-grained sand that probably accumulated during the annual spring snowmelt), characterized by an abundance of Meesia triquetra and a dominance of benthic diatoms (e. g. Navicula vulpina), indicative of a relatively high pH and a high tolerance of disturbance. A comparison of the local polygon vegetation (inferred from moss and macrofossil spectra) with the regional vegetation (inferred from pollen spectra) indicated that the moss association with Scorpidium scorpioides became established during relatively favourable climatic conditions, while the association dominated by Meesia triquetra occurred during periods of harsh climatic conditions. Our study revealed a strong riverine influence (in addition to climatic influences) on polygon development and the type of peat accumulated.
The digital laser rangefinder GLM Professional (R) BOSCH 250 VF was tested as a modified preproduction model with regard to its applicability of quantifying humans' height. The aim of this investigation was to determine and evaluate the instrument's precision, as well as its manageability within anthropometric field studies. Data collected by the digital laser rangefinder did not show a significant difference to data of control by an anthropometer. Furthermore, more than 96% of the difference values are located within area of agreement. Nevertheless, the GLM Professional (R) is a highly sensitive instrument and mean SD within threefold data acquisition is twice as high as SD resulting from data collection by an anthropometer. However, due to the minimal percentage differences within data acquisition and compared to the standard method, the GLM Professional (R) is proved to be a reliable instrument and to be highly applicable for anthropometric field studies. Furthermore, due to its excellent manageability and compact size, the GLM Professional (R) shows a very good applicability even for less trained anthropometrists and thus ameliorates the possibilities of collecting reliable data within anthropometric field studies.
We demonstrate new fluorophore-labelled materials based on acrylamide and on oligo(ethylene glycol) (OEG) bearing thermoresponsive polymers for sensing purposes and investigate their thermally induced solubility transitions. It is found that the emission properties of the polarity-sensitive (solvatochromic) naphthalimide derivative attached to three different thermoresponsive polymers are highly specific to the exact chemical structure of the macromolecule. While the dye emits very weakly below the LCST when incorporated into poly(N-isopropylacrylamide) (pNIPAm) or into a polyacrylate backbone bearing only short OEG side chains, it is strongly emissive in polymethacrylates with longer OEG side chains. Heating of the aqueous solutions above their cloud point provokes an abrupt increase of the fluorescence intensity of the labelled pNIPAm, whereas the emission properties of the dye are rather unaffected as OEG-based polyacrylates and methacrylates undergo phase transition. Correlated with laser light scattering studies, these findings are ascribed to the different degrees of pre-aggregation of the chains at low temperatures and to the extent of dehydration that the phase transition evokes. It is concluded that although the temperature-triggered changes in the macroscopic absorption characteristics, related to large-scale alterations of the polymer chain conformation and aggregation, are well detectable and similar for these LCST-type polymers, the micro-environment provided to the dye within each polymer network differs substantially. Considering sensing applications, this finding is of great importance since the temperature-regulated fluorescence response of the polymer depends more on the macromolecular architecture than the type of reporter fluorophore.
Sugar-based molecules and polysaccharide biomass can be turned into porous functional carbonaceous products at comparably low temperatures of 400 degrees C under a nitrogen atmosphere in the presence of an ionic liquid (IL) or a poly(ionic liquid) (PIL). The IL and PIL act as "activation agents" with own structural contribution, and effectively promote the conversion and pore generation in the biomaterials even at a rather low doping ratio (7 wt%). In addition, this "induced carbonization" and pore forming phenomenon enables the preservation of the biotemplate shape to the highest extent and was employed to fabricate shaped porous carbonaceous materials from carbohydrate-based biotemplates, exemplified here with cellulose filter membranes, coffee filter paper and natural cotton. These carbonized hybrids exhibit comparably good mechanical properties, such as bendability of membranes or shape recovery of foams. Moreover, the nitrogen atoms incorporated in the final products from the IL/PIL precursors further improve the oxidation stability in the fire-retardant tests.
Magnetic field generation in a jet-sheath plasma via the kinetic Kelvin-Helmholtz instability
(2013)
We have investigated the generation of magnetic fields associated with velocity shear between an unmagnetized relativistic jet and an unmagnetized sheath plasma. We have examined the strong magnetic fields generated by kinetic shear (Kelvin-Helmholtz) instabilities. Compared to the previous studies using counter-streaming performed by Alves et al. (2012), the structure of the kinetic Kelvin-Helmholtz instability (KKHI) of our jet-sheath configuration is slightly different, even for the global evolution of the strong transverse magnetic field. In our simulations the major components of growing modes are the electric field E-z, perpendicular to the flow boundary, and the magnetic field B-y, transverse to the flow direction. After the B-y component is excited, an induced electric field E-x, parallel to the flow direction, becomes significant. However, other field components remain small. We find that the structure and growth rate of KKHI with mass ratios m(i)/m(e) = 1836 and m(i)/m(e) = 20 are similar. In our simulations in the nonlinear stage is not as clear as in counter-streaming cases. The growth rate for a mildly-relativistic jet case (gamma(j) = 1.5) is larger than for a relativistic jet case (gamma(j) = 15).
Sustainable land use in Mountain Regions under global change synthesis across scales and disciplines
(2013)
Mountain regions provide essential ecosystem goods and services (EGS) for both mountain dwellers and people living outside these areas. Global change endangers the capacity of mountain ecosystems to provide key services. The Mountland project focused on three case study regions in the Swiss Alps and aimed to propose land-use practices and alternative policy solutions to ensure the provision of key EGS under climate and land-use changes. We summarized and synthesized the results of the project and provide insights into the ecological, socioeconomic, and political processes relevant for analyzing global change impacts on a European mountain region. In Mountland, an integrative approach was applied, combining methods from economics and the political and natural sciences to analyze ecosystem functioning from a holistic human-environment system perspective. In general, surveys, experiments, and model results revealed that climate and socioeconomic changes are likely to increase the vulnerability of the EGS analyzed. We regard the following key characteristics of coupled human-environment systems as central to our case study areas in mountain regions: thresholds, heterogeneity, trade-offs, and feedback. Our results suggest that the institutional framework should be strengthened in a way that better addresses these characteristics, allowing for (1) more integrative approaches, (2) a more network-oriented management and steering of political processes that integrate local stakeholders, and (3) enhanced capacity building to decrease the identified vulnerability as central elements in the policy process. Further, to maintain and support the future provision of EGS in mountain regions, policy making should also focus on project-oriented, cross-sectoral policies and spatial planning as a coordination instrument for land use in general.
We consider diffusion processes with a spatially varying diffusivity giving rise to anomalous diffusion. Such heterogeneous diffusion processes are analysed for the cases of exponential, power-law, and logarithmic dependencies of the diffusion coefficient on the particle position. Combining analytical approaches with stochastic simulations, we show that the functional form of the space-dependent diffusion coefficient and the initial conditions of the diffusing particles are vital for their statistical and ergodic properties. In all three cases a weak ergodicity breaking between the time and ensemble averaged mean squared displacements is observed. We also demonstrate a population splitting of the time averaged traces into fast and slow diffusers for the case of exponential variation of the diffusivity as well as a particle trapping in the case of the logarithmic diffusivity. Our analysis is complemented by the quantitative study of the space coverage, the diffusive spreading of the probability density, as well as the survival probability.
A water soluble fluorescent polymer as a dual colour sensor for temperature and a specific protein
(2013)
We present two thermoresponsive water soluble copolymers prepared via free radical statistical copolymerization of N-isopropylacrylamide (NIPAm) and of oligo(ethylene glycol) methacrylates (OEGMAs), respectively, with a solvatochromic 7-(diethylamino)-3-carboxy-coumarin (DEAC)-functionalized monomer. In aqueous solutions, the NIPAm-based copolymer exhibits characteristic changes in its fluorescence profile in response to a change in solution temperature as well as to the presence of a specific protein, namely an anti-DEAC antibody. This polymer emits only weakly at low temperatures, but exhibits a marked fluorescence enhancement accompanied by a change in its emission colour when heated above its cloud point. Such drastic changes in the fluorescence and absorbance spectra are observed also upon injection of the anti-DEAC antibody, attributed to the specific binding of the antibody to DEAC moieties. Importantly, protein binding occurs exclusively when the polymer is in the well hydrated state below the cloud point, enabling a temperature control on the molecular recognition event. On the other hand, heating of the polymer-antibody complexes releases a fraction of the bound antibody. In the presence of the DEAC-functionalized monomer in this mixture, the released antibody competitively binds to the monomer and the antibody-free chains of the polymer undergo a more effective collapse and inter-aggregation. In contrast, the emission properties of the OEGMA-based analogous copolymer are rather insensitive to the thermally induced phase transition or to antibody binding. These opposite behaviours underline the need for a carefully tailored molecular design of responsive polymers aimed at specific applications, such as biosensing.
A rare example of in situ linker generation with the formation of soft porous Zn- and Co-MOFs (IFP-9 and -10, respectively) is reported. The flexible ethoxy groups of IFP-9 and -10 protrude into the 1D hexagonal channels. The gas-sorption behavior of both materials for H-2, CO2 and CH4 showed wide hysteretic isotherms, typical for MOFs having a flexible substituent which can give rise to a gate effect.
Control of fixation duration during scene viewing by interaction of foveal and peripheral processing
(2013)
Processing in our visual system is functionally segregated, with the fovea specialized in processing fine detail (high spatial frequencies) for object identification, and the periphery in processing coarse information (low frequencies) for spatial orienting and saccade target selection. Here we investigate the consequences of this functional segregation for the control of fixation durations during scene viewing. Using gaze-contingent displays, we applied high-pass or low-pass filters to either the central or the peripheral visual field and compared eye-movement patterns with an unfiltered control condition. In contrast with predictions from functional segregation, fixation durations were unaffected when the critical information for vision was strongly attenuated (foveal low-pass and peripheral high-pass filtering); fixation durations increased, however, when useful information was left mostly intact by the filter (foveal high-pass and peripheral low-pass filtering). These patterns of results are difficult to explain under the assumption that fixation durations are controlled by foveal processing difficulty. As an alternative explanation, we developed the hypothesis that the interaction of foveal and peripheral processing controls fixation duration. To investigate the viability of this explanation, we implemented a computational model with two compartments, approximating spatial aspects of processing by foveal and peripheral activations that change according to a small set of dynamical rules. The model reproduced distributions of fixation durations from all experimental conditions by variation of few parameters that were affected by specific filtering conditions.
Whenever eye movements are measured, a central part of the analysis has to do with where subjects fixate and why they fixated where they fixated. To a first approximation, a set of fixations can be viewed as a set of points in space; this implies that fixations are spatial data and that the analysis of fixation locations can be beneficially thought of as a spatial statistics problem. We argue that thinking of fixation locations as arising from point processes is a very fruitful framework for eye-movement data, helping turn qualitative questions into quantitative ones. We provide a tutorial introduction to some of the main ideas of the field of spatial statistics, focusing especially on spatial Poisson processes. We show how point processes help relate image properties to fixation locations. In particular we show how point processes naturally express the idea that image features' predictability for fixations may vary from one image to another. We review other methods of analysis used in the literature, show how they relate to point process theory, and argue that thinking in terms of point processes substantially extends the range of analyses that can be performed and clarify their interpretation.
Intracellular photoactivation of caged cGMP induces myosin II and actin responses in motile cells
(2013)
Cyclic GMP (cGMP) is a ubiquitous second messenger in eukaryotic cells. It is assumed to regulate the association of myosin II with the cytoskeleton of motile cells. When cells of the social amoeba Dictyostelium discoideum are exposed to chemoattractants or to increased osmotic stress, intracellular cGMP levels rise, preceding the accumulation of myosin II in the cell cortex. To directly investigate the impact of intracellular cGMP on cytoskeletal dynamics in a living cell, we released cGMP inside the cell by laser-induced photo-cleavage of a caged precursor. With this approach, we could directly show in a live cell experiment that an increase in intracellular cGMP indeed induces myosin II to accumulate in the cortex. Unexpectedly, we observed for the first time that also the amount of filamentous actin in the cell cortex increases upon a rise in the cGMP concentration, independently of cAMP receptor activation and signaling. We discuss our results in the light of recent work on the cGMP signaling pathway and suggest possible links between cGMP signaling and the actin system.
Due to limited public budgets and the need to economize, the analysis of costs of hazard mitigation and emergency management of natural hazards becomes increasingly important for public natural hazard and risk management. In recent years there has been a growing body of literature on the estimation of losses which supported to help to determine benefits of measures in terms of prevented losses. On the contrary, the costs of mitigation are hardly addressed. This paper thus aims to shed some light on expenses for mitigation and emergency services. For this, we analysed the annual costs of mitigation efforts in four regions/countries of the Alpine Arc: Bavaria (Germany), Tyrol (Austria), South Tyrol (Italy) and Switzerland. On the basis of PPP values (purchasing power parities), annual expenses on public safety ranged from EUR 44 per capita in the Free State of Bavaria to EUR 216 in the Autonomous Province of South Tyrol. To analyse the (variable) costs for emergency services in case of an event, we used detailed data from the 2005 floods in the Federal State of Tyrol (Austria) as well as aggregated data from the 2002 floods in Germany. The analysis revealed that multi-hazards, the occurrence and intermixture of different natural hazard processes, contribute to increasing emergency costs. Based on these findings, research gaps and recommendations for costing Alpine natural hazards are discussed.
Flood loss modeling is an important component within flood risk assessments. Traditionally, stage-damage functions are used for the estimation of direct monetary damage to buildings. Although it is known that such functions are governed by large uncertainties, they are commonly applied - even in different geographical regions - without further validation, mainly due to the lack of real damage data. Until now, little research has been done to investigate the applicability and transferability of such damage models to other regions. In this study, the last severe flood event in the Austrian Lech Valley in 2005 was simulated to test the performance of various damage functions from different geographical regions in Central Europe for the residential sector. In addition to common stage-damage curves, new functions were derived from empirical flood loss data collected in the aftermath of recent flood events in neighboring Germany. Furthermore, a multi-parameter flood loss model for the residential sector was adapted to the study area and also evaluated with official damage data. The analysis reveals that flood loss functions derived from related and more similar regions perform considerably better than those from more heterogeneous data sets of different regions and flood events. While former loss functions estimate the observed damage well, the latter overestimate the reported loss clearly. To illustrate the effect of model choice on the resulting uncertainty of damage estimates, the current flood risk for residential areas was calculated. In the case of extreme events like the 300 yr flood, for example, the range of losses to residential buildings between the highest and the lowest estimates amounts to a factor of 18, in contrast to properly validated models with a factor of 2.3. Even if the risk analysis is only performed for residential areas, our results reveal evidently that a carefree model transfer in other geographical regions might be critical. Therefore, we conclude that loss models should at least be selected or derived from related regions with similar flood and building characteristics, as far as no model validation is possible. To further increase the general reliability of flood loss assessment in the future, more loss data and more comprehensive loss data for model development and validation are needed.
Background/Aims: Carbon monoxide (CO) interferes with cytochrome-dependent cellular functions and acts as gaseous transmitter. CO is released from CO-releasing molecules (CORM) including tricarbonyl-dichlororuthenium (II) dimer (CORM-2), molecules considered for the treatment of several disorders including vascular dysfunction, inflammation, tissue ischemia and organ rejection. Cytochrome P450-sensitive function include formation of 1,25-dihydroxyvitamin D-3 (1,25(OH)(2)D-3) by renal 25-hydroxyvitamin D-3 1-alpha-hydroxylase (Cyp27b1). The enzyme is regulated by PTH, FGF23 and klotho. 1,25(OH)(2)D-3 regulates Ca2+ and phosphate transport as well as klotho expression. The present study explored, whether CORM-2 influences 1,25(OH)(2)D-3 formation and klotho expression. Methods: Mice were treated with intravenous CORM-2 (20 mg/kg body weight). Plasma 1,25(OH)(2)D-3 and FGF23 concentrations were determined by ELISA, phosphate, calcium and creatinine concentrations by colorimetric methods, transcript levels by quantitative RT-PCR and protein expression by western blotting. Fgf23 mRNA transcript levels were further determined in rat osteosarcoma UMR106 cells without or with prior treatment for 24 hours with 20 mu M CORM-2. Results: CORM-2 injection within 24 hours significantly increased FGF23 plasma levels and decreased 1,25(OH)(2)D-3 plasma levels, renal Cyp27b1 gene expression as well as renal klotho protein abundance and transcript levels. Moreover, treatment of UMR106 cells with CORM-2 significantly increased Fgf23 transcript levels. Conclusion: CO-releasing molecule CORM-2 enhances FGF23 expression and release and decreases klotho expression and 1,25(OH)(2)D-3 synthesis.
Background: The biological properties of oxidized and non-oxidized PTH are substantially different. Oxidized PTH (oxPTH) loses its PTH receptor-stimulating properties, whereas non-oxidized PTH (n-oxPTH) is a full agonist of the receptor. This was described in more than 20 well published studies in the 1970(s) and 80(s). However, PTH oxidation has been ignored during the development of PTH assays for clinical use so far. Even the nowadays used third generation assay systems do not consider oxidation of PTH. We recently developed an assay to differentiate between oxPTH and n-oxPTH. In the current study we established normal values for this assay system. Furthermore, we compare the ratio of oxPTH to n-oxPTH in different population with chronic renal failure: 620 children with renal failure stage 2-4 of the 4C study, 342 adult patients on dialysis, and 602 kidney transplant recipients. In addition, we performed modeling of the interaction of either oxPTH or n-oxPTH with the PTH receptor using biophysical structure approaches. Results: The children had the highest mean as well as maximum n-oxPTH concentrations as compared to adult patients (both patients on dialysis as well as kidney transplant recipients). The relationship between oxPTH and n-oxPTH of individual patients varied substantially in all three populations with renal impairment. The analysis of n-oxPTH in 89 healthy control subjects revealed that n-oxPTH concentrations in patient with renal failure were higher as compared to healthy adult controls (2.25-fold in children with renal failure, 1.53-fold in adult patients on dialysis, and 1.56-fold in kidney transplant recipients, respectively). Computer assisted biophysical structure modeling demonstrated, however, minor sterical- and/or electrostatic changes in oxPTH and n-oxPTH. This indicated that PTH oxidation may induce refolding of PTH and hence alters PTH-PTH receptor interaction via oxidation induced three-dimensional structure alteration of PTH. Conclusion: A huge proportion of circulating PTH measured by current state-of-the-art assay systems is oxidized and thus not biologically active. The relationship between oxPTH and n-oxPTH of individual patients varied substantially. Non-oxidized PTH concentrations are 1.5 - 2.25 fold higher in patients with renal failure as compared to health controls. Measurements of n-oxPTH may reflect the hormone status more precise. The iPTH measures describes most likely oxidative stress in patients with renal failure rather than the PTH hormone status. This, however, needs to be demonstrated in further clinical studies.
Eye-movement research on implicit prosody has found effects of lexical stress on syntactic ambiguity resolution, suggesting that metrical well-formedness constraints interact with syntactic category assignment. Building on these findings, the present eyetracking study investigates whether contextual bias can modulate the effects of metrical structure on syntactic ambiguity resolution in silent reading. Contextual bias and potential stress-clash in the ambiguous region were crossed in a 2 x 2 design. Participants read biased context sentences followed by temporarily ambiguous test sentences. In the three-word ambiguous region, main effects of lexical stress were dominant, while early effects of context were absent. Potential stress clash yielded a significant increase in first-pass regressions and re-reading probability across the three words. In the disambiguating region, the disambiguating word itself showed increased processing difficulty (lower skipping and increased re-reading probability) when the disambiguation engendered a stress clash configuration, while the word immediately following showed main effects of context in those same measures. Taken together, effects of lexical stress upon eye movements were swift and pervasive across first-pass and second-pass measures, while effects of context were relatively delayed. These results indicate a strong role for implicit meter in guiding parsing, one that appears insensitive to higher-level constraints. Our findings are problematic for two classes of models, the two-stage garden-path model and the constraint-based competition-integration model, but can be explained by a variation on the two-stage model, the unrestricted race model.
Sightseeing in the poorest quarters of southern hemisphere cities has been observed occurring in Cape Town, Rio de Janeiro, Mumbai and many other cities. The increasing global interest in touring poor urban environments is accompanied by a strong morally charged debate; so far, this debate has not been critically addressed. This article avoids asking if slum tourism is good or bad, but instead seeks a second-order observation, i.e. to investigate under what conditions the social praxis of slum tourism is considered as good or bad, by processing information on esteem or dis-esteem among tourists and tour providers. Special attention is given to any relation between morality and place, and the thesis posited is that the moral charging of slum tourism is dependent on the presence of specific preconceived notions of slums and poverty. This shall be clarified by means of references to two empirical case studies carried out in (1) Cape Town in 2007 and 2008 and (2) Mumbai in 2009.
BACKGROUND: Mirror therapy (MT) was found to improve motor function after stroke, but its neural mechanisms remain unclear, especially in single stroke patients.
OBJECTIVES: The following imaging study was designed to compare brain activation patterns evoked by the mirror illusion in single stroke patients with normal subjects.
METHODS: Fifteen normal volunteers and five stroke patients with severe arm paresis were recruited. Cerebral activations during movement mirroring by means of a video chain were recorded with functional magnetic resonance imaging (fMRI). Single-subject analysis was performed using SPM 8.
RESULTS: For normal subjects, ten and thirteen subjects displayed lateralized cerebral activations evoked by the mirror illusion while moving their right and left hand respectively. The magnitude of this effect in the precuneus contralateral to the seen hand was not dependent on movement speed or subjective experience. Negative correlation of activation strength with age was found for the right hand only. The activation pattern in stroke patients is comparable to that of normal subjects and present in four out of five patients.
CONCLUSIONS: In summary, the mirror illusion can elicit cerebral activation contralateral to the perceived hand in the majority of single normal subjects, but not in all of them. This is similar even in stroke patients with severe hemiparesis.
Changes in rainfall interception along a secondary forest succession gradient in lowland Panama
(2013)
Secondary forests are rapidly expanding in tropical regions. Yet, despite the importance of understanding the hydrological consequences of land-cover dynamics, the relationship between forest succession and canopy interception is poorly understood. This lack of knowledge is unfortunate because rainfall interception plays an important role in regional water cycles and needs to be quantified for many modeling purposes. To help close this knowledge gap, we designed a throughfall monitoring study along a secondary succession gradient in a tropical forest region of Panama. The investigated gradient comprised 20 forest patches 3 to 130 yr old. We sampled each patch with a minimum of 20 funnel-type throughfall collectors over a continuous 2month period that had nearly 900 mm of rain. During the same period, we acquired forest inventory data and derived several forest structural attributes. We then applied simple and multiple regression models (Bayesian model averaging, BMA) and identified those vegetation parameters that had the strongest influence on the variation of canopy interception. Our analyses yielded three main findings. First, canopy interception changed rapidly during forest succession. After only a decade, throughfall volumes approached levels that are typical for mature forests. Second, a parsimonious (simple linear regression) model based on the ratio of the basal area of small stems to the total basal area outperformed more complex multivariate models (BMA approach). Third, based on complementary forest inventory data, we show that the influence of young secondary forests on interception in realworld fragmented landscapes might be detectable only in regions with a substantial fraction of young forests. Our re-sults suggest that where entire catchments undergo forest regrowth, initial stages of succession may be associated with a substantial decrease of streamflow generation. Our results further highlight the need to study hydrological processes in all forest succession stages, including early ones.
The dynamics of external contributions to the geomagnetic field is investigated by applying time-frequency methods to magnetic observatory data. Fractal models and multiscale analysis enable obtaining maximum quantitative information related to the short-term dynamics of the geomagnetic field activity. The stochastic properties of the horizontal component of the transient external field are determined by searching for scaling laws in the power spectra. The spectrum fits a power law with a scaling exponent beta, a typical characteristic of self-affine time-series. Local variations in the power-law exponent are investigated by applying wavelet analysis to the same time-series. These analyses highlight the self-affine properties of geomagnetic perturbations and their persistence. Moreover, they show that the main phases of sudden storm disturbances are uniquely characterized by a scaling exponent varying between 1 and 3, possibly related to the energy contained in the external field. These new findings suggest the existence of a long-range dependence, the scaling exponent being an efficient indicator of geomagnetic activity and singularity detection. These results show that by using magnetogram regularity to reflect the magnetosphere activity, a theoretical analysis of the external geomagnetic field based on local power-law exponents is possible.
Background: Low birth weight (LBW) might be a risk factor for acquiring lower respiratory tract infections (LRTIs) associated with disease related complications in early childhood. HFMD, a frequent viral infection in southern China, is a leading cause of lower respiratory tract infections in children. We analyzed whether LBW is a risk factor for children with HFMD to develop lower respiratory tract infections.
Methods: A total of 298 children with HFMD, admitted to a hospital in Qingyuan city, Guangdong province, were recruited. Demographic data and clinical parameters such as serum glucose level and inflammatory markers including peripheral white blood cell count, serum C-reactive protein, and erythrocyte sedimentation rate were routinely collected on admission. Birth weight data were derived from birth records.
Results: Mean birth weight (BW) was 167 g lower in patients with HFMD and LRTIs as compared to patients with solely HFMD (p = 0.022) and the frequency of birth weight below the tenth percentile was significantly higher in patients with HFMD and LRTIs (p = 0.002).
Conclusions: The results of the study show that low birth weight is associated with a higher incidence of lower respiratory tract infections in young children with HFMD.
Quantifying the association of plant functional traits to environmental gradients is a promising approach for understanding and projecting community responses to land use and climatic changes. Although habitat fragmentation and climate are expected to affect plant communities interactively, there is a lack of empirical studies addressing trait associations to fragmentation in different climatic regimes.
In this study, we analyse data on the key functional traits: specific leaf area (SLA), plant height, seed mass and seed number. First, we assess the evidence for the community assembly mechanisms habitat filtering and competition at different spatial scales, using several null-models and a comprehensive set of community-level trait convergence and divergence indices. Second, we analyse the association of community-mean traits with patch area and connectivity along a south-north productivity gradient.
We found clear evidence for trait convergence due to habitat filtering. In contrast, the evidence for trait divergence due to competition fundamentally depended on the null-model used. When the null-model controlled for habitat filtering, there was only evidence for trait divergence at the smallest sampling scale (0.25 m x 0.25 m). All traits varied significantly along the S-N productivity gradient. While plant height and SLA were consistently associated with fragmentation, the association of seed mass and seed number with fragmentation changed along the S-N gradient.
Our findings indicate trait convergence due to drought stress in the arid sites and due to higher productivity in the mesic sites. The association of plant traits to fragmentation is likely driven by increased colonization ability in small and/or isolated patches (plant height, seed number) or increased persistence ability in isolated patches (seed mass).
Our study provides the first empirical test of trait associations with fragmentation along a productivity gradient. We conclude that it is crucial to study the interactive effects of different ecological drivers on plant functional traits.
The possibility of an impact of global warming on the Indian monsoon is of critical importance for the large population of this region. Future projections within the Coupled Model Intercomparison Project Phase 3 (CMIP-3) showed a wide range of trends with varying magnitude and sign across models. Here the Indian summer monsoon rainfall is evaluated in 20 CMIP-5 models for the period 1850 to 2100. In the new generation of climate models, a consistent increase in seasonal mean rainfall during the summer monsoon periods arises. All models simulate stronger seasonal mean rainfall in the future compared to the historic period under the strongest warming scenario RCP-8.5. Increase in seasonal mean rainfall is the largest for the RCP-8.5 scenario compared to other RCPs. Most of the models show a northward shift in monsoon circulation by the end of the 21st century compared to the historic period under the RCP-8.5 scenario. The interannual variability of the Indian monsoon rainfall also shows a consistent positive trend under unabated global warming. Since both the long-term increase in monsoon rainfall as well as the increase in interannual variability in the future is robust across a wide range of models, some confidence can be attributed to these projected trends.
Intimate partner violence as a global problem - international and interdisciplinary perspectives
(2013)
This editorial introduces the Focus Section on Intimate Partner Violence (IPV) as a worldwide problem, which brings together six papers that are truly international and interdisciplinary. They provide insights into IPV from nine different cultures - China, Germany, Italy, Japan, Mexico, Northern Ireland, Sweden, Turkey, and the United States - from scholars in the fields of psychology, gender studies, political science, and economics. The first three papers look at how widespread the experience of IPV is among different groups of women, examine selected risk factors associated with heightened vulnerability to victimization, and discuss consequences of intimate partner victimization. Another two papers place the problem of IPV in the wider context of societal perceptions and attitudes about victims and perpetrators of IPV in different countries, whereas the last paper examines the role of individual differences in the management of emotions in the escalation or de-escalation of relationship conflict. In combination, the papers highlight the interplay between the macro level of social and cultural norms condoning the use of violence, the micro level of family relations and construction of couple relationships, and the individual level of attitudes and behaviors that precipitate IPV.
Transcriptome analysis through next-generation sequencing technologies allows the generation of detailed gene catalogs for non-model species, at the cost of new challenges with regards to computational requirements and bioinformatics expertise. Here, we present TRAPID, an online tool for the fast and efficient processing of assembled RNA-Seq transcriptome data, developed to mitigate these challenges. TRAPID offers high-throughput open reading frame detection, frameshift correction and includes a functional, comparative and phylogenetic toolbox, making use of 175 reference proteomes. Benchmarking and comparison against state-of-the-art transcript analysis tools reveals the efficiency and unique features of the TRAPID system.
Polymers exhibiting cell-selective effects represent an extensive research field with high relevance for biomedical applications e.g. in the cardiovascular field supporting re-endothelialization while suppressing smooth muscle cell overgrowth. Such an endothelial cell-selective effect could be recently demonstrated for a copolyetheresterurethane (PDC) containing biodegradable poly(p-dioxanone) and poly(epsilon-caprolactone) segments, which selectively enhanced the adhesion of human umbilical vein endothelial cells (HUVEC) while suppressing the attachment of smooth muscle cells (SMC).
In this study we investigated the influence of the fibre orientation (random and aligned) and fibre diameter (2 mu m and 500 nm) of electrospun PDC scaffolds on the adhesion, proliferation and apoptosis of HUVEC and SMC.
Adhesion, viability and proliferation of HUVEC was diminished when the fibre diameter was reduced to a submicron scale, while the orientation of the microfibres did only slightly influence the cellular behaviour. In contrast, a submicron fibre diameter improved SMC viability. In conclusion, PDC scaffolds with micron-sized single fibres could be promising candidate materials for cell-selective stent coatings.
Invasive alien species pose a great threat to the integrity of natural communities by competition with and predation on native species. In Germany the invasive raccoon dog (Nyctereutes procyonoides) and the native red fox (Vulpes vulpes) occupy a similar ecological niche. Therefore, the aim of our study was to discover the extent of exploitative diet competition between these two generalist carnivores. Carcasses of red foxes (n=256) and raccoon dogs (n=253) were collected throughout Mecklenburg Western-Pomerania (north-east Germany) and stomachs contains were analysed. Frequency of occurrence and biomass share indicate that both canids are omnivorous and pursue opportunistic feeding strategies. Small mammals and edible plant material were the most important food resources for red foxes and raccoon dogs. Nonetheless, interspecies differences were recorded for edible plant material, small mammals and insects. While red foxes mostly feed on voles, raccoon dogs consumed mice and shrews as often as voles. Only raccoon dogs preyed on amphibians. There were no differences in carrion consumption, both species scavenged on wild boar and we found clear competition for carrion year-round. Moreover, there was evidence that two red foxes foraged on raccoon dogs and vice versa. The mean annual interspecies diet overlap index was relatively high. The diets determined for raccoon dogs and red foxes were quite similar and a similar food niche breadth was recorded. However, only minor competition is assumed to take place since differences in feeding habits do exist.