publish.UP Search

Overt language production of German past participles (2018)

Marusch, Tina ; Jäger, Lena A. ; Neiß, Leander ; Burchert, Frank ; Nickels, Lyndsey

We report two experiments and Bayesian modelling of the data collected. In both experiments, participants performed a long-lag primed picture naming task. Black-and-white line drawings were used as targets, which were overtly named by the participants. Their naming latencies were measured. In both experiments, primes consisted of past participle verbs (er tanzt/er hat getanzt “he dances/he has danced”) and the relationship between primes and targets was either morphological or unrelated. Experiment 1 additionally had phonologically and semantically related prime-target pairs as well as present tense primes. Both in Experiment 1 and 2, participants showed significantly faster naming latencies for morphologically related targets relative to the unrelated verb primes. In Experiment 1, no priming effects were observed in phonologically and semantically related control conditions. In addition, the production latencies were not influenced by verb type.

Thematic role assignment in the L1 acquisition of Tagalog (2018)

Garcia, Rowena ; Roeser, Jens ; Höhle, Barbara

It is a common finding across languages that young children have problems in understanding patient-initial sentences. We used Tagalog, a verb-initial language with a reliable voice-marking system and highly frequent patient voice constructions, to test the predictions of several accounts that have been proposed to explain this difficulty: the frequency account, the Competition Model, and the incremental processing account. Study 1 presents an analysis of Tagalog child-directed speech, which showed that the dominant argument order is agent-before-patient and that morphosyntactic markers are highly valid cues to thematic role assignment. In Study 2, we used a combined self-paced listening and picture verification task to test how Tagalog-speaking adults and 5- and 7-year-old children process reversible transitive sentences. Results showed that adults performed well in all conditions, while children's accuracy and listening times for the first noun phrase indicated more difficulty in interpreting patient-initial sentences in the agent voice compared to the patient voice. The patient voice advantage is partly explained by both the frequency account and incremental processing account.

Do mental health patients learn what their cognitive-behaviour therapists think they do? (2018)

Kühne, Franziska ; Lesser, Hannah ; Petri, Franziska ; Weck, Florian

Purpose: The acquisition of skills is essential to the conceptualization of cognitive-behavioural therapy. Yet, what experiences are encountered and what skills actually learned during therapy, and whether patients and therapists have concurrent views hereof, remains poorly understood. Method: An explorative pilot study with semi-structured, corresponding interview guides was conducted. Pilot data from our outpatient unit were transcribed and content-analyzed following current guidelines. Results: The responses of 18 participants (patients and their psychotherapists) were assigned to six main categories. Educational and cognitive aspects were mentioned most frequently and consistently by both groups. Having learned Behavioural alternatives attained the second highest agreement between perspectives. Conclusions: Patients and therapists valued CBT as an opportunity to learn new skills, which is an important prerequisite also for the maintenance of therapeutic change. We discuss limitations to generalizability but also theoretical and therapy implications.

Territorial reforms in Europe (2018)

Ebinger, Falk ; Kuhlmann, Sabine ; Bogumil, Joerg

Territorial reform is the most radical and contested reorganisation of local government. A sound evaluation of the outcome of such reforms is hence an important step to ensure the legitimation of any decision on the subject. However, in our view the discourse on the subject appears to be one sided, focusing primarily on overall fiscal effects scrutinised by economists. The contribution of this paper is hence threefold: Firstly, we provide an overview off territorial reforms in Europe, with a special focus on Eastern Germany as a promising case for cross-country comparisons. Secondly, we provide an over-view of the analytical classifications of these reforms and context factors to be considered in their evaluation. And thirdly, we analyse the literature on qualitative performance effects of these reforms. The results show that territorial reforms have a significant positive impact on functional performance, while the effects on participation and integration are indeed ambivalent. In doing so, we provide substantial arguments for a broader, more inclusive discussion on the success of territorial reforms.

Immunscreening Virulenz-adaptierter Expressionsbibliotheken aus einem in vitro Infektionsmodell mit Salmonella Enteritidis (2018)

Danckert, Lena

Die Folgen einer lebensmittelbedingten Erkrankung sind zum Teil gravierend, insbesondere für Kinder und immunsupprimierte Menschen. Hierbei gehören Salmonella und Campylobacter zu den häufigsten Erregern, die verantwortlich für gastrointestinale Erkrankungen in Deutschland sind. Trotz umfassender Maßnahmen der EU zur Prävention und Bekämpfung von Salmonellen in Geflügelbeständen und der Lebensmittel-Industrie, wird von einem stagnierenden Trend von Infektionszahlen berichtet. Zoonose-Erreger wie Salmonellen können über Nutztiere in die Nahrungskette des Menschen gelangen, wodurch sich Infektionsherde schnell ausbreiten können. Dabei sind bestehende Präventionsstrategien für Geflügel vorhanden, die aber nicht auf den Menschen übertragbar sind. Folglich sind Diagnostik und Prävention in der Lebensmittelindustrie essentiell. Deshalb besteht ein hoher Bedarf für spezifische, sensitive und zuverlässige Nachweismethoden, die eine Point-of-care Diagnostik gewährleisten. Durch ein wachsendes Verständnis der wirtsspezifischen Faktoren von S. enterica Serovaren kann die Entwicklung sowohl neuartiger diagnostischer Methoden, als auch neuartiger Therapien und Impfstoffe maßgeblich vorangetrieben werden. Infolgedessen wurde in dieser Arbeit ein infektionsähnliches in vitro Modell für S. Enteritidis etabliert und darauf basierend eine umfassende Untersuchung zur Identifizierung neuer Zielstrukturen für den Erreger durchgeführt. Während einer Salmonellen-Infektion ist die erste zelluläre Barriere im Wirt die Epithelschicht. Dementsprechend wurde eine humane Zelllinie (CaCo 2, Darmepithel) für die Pathogen-Wirt-Studie ausgewählt. Das Salmonellen-Transkriptom und morphologische Eigenschaften der Epithelzellen wurden in verschiedenen Phasen der Salmonellen-Infektion untersucht und mit bereits gut beschriebenen Virulenzfaktoren und Beobachtungen in Bezug gesetzt. Durch dieses Infektionsmodell konnte ein spezifischer Phänotyp für die intrazellulären Salmonellen in den Epithelzellen nachgewiesen werden. Zudem wurde aufgezeigt, dass bereits die Kultivierung in Flüssigmedium einen invasionsaktiven Zustand der Salmonellen erzeugt. Allerdings wurde durch die Kokultivierung mit Epithelzellen eine zusätzliche Expression relevanter Gene induziert, um eine effiziente Adhäsion und Transmembran-Transport zu gewährleisten. Letzterer ist charakteristisch für die intrazelluläre Limitierung von Nährstoffen und prägt den infektionsrelevanten Status. Unter Berücksichtigung dieser Faktoren ergab sich ein Phänotyp, der eindeutig Mechanismen zur Wirtsadaptation und möglicherweise auch Pathogenese aufzeigt. Die intrazellulären Bakterien müssen vom Wirt separiert werden, was ein wesentlicher Schritt für Pathogen-bestimmende Analysen ist. Hierbei wurde mithilfe einer Detergenz-basierten Lyse der eukaryotischen Zellmembran und differentieller Zentrifugation, der eukaryotische Eintrag minimal gehalten. Unter Verwendung der Virulenz-adaptierten Salmonellen wurden Untersuchungen in Hinblick auf die Identifizierung neuer Zielstrukturen für S. Enteritidis durchgeführt. Mithilfe eines immunologischen Screenings wurden neue potentielle Antigene entdeckt. Zu diesem Zweck wurden bakterielle cDNA-basierte Expressionsbibliotheken hergestellt, die durch eine vereinfachte Microarray-Anwendung ein Hochdurchsatzscreening von Proteinen als potentielle Binder ermöglichen. Folglich konnten neue unbeschriebene Proteine identifiziert werden, die sich durch eine Salmonella-Spezifität oder Membranständigkeit auszeichnen. Ebenso wurde ein Vergleich der im Screening identifizierten Proteine mit der Regulation der kodierenden Gene im infektionsähnlichen Modell durchgeführt. Dabei wurde deutlich, dass die Häufigkeit von Transkripten einen Einfluss auf die Verfügbarkeit in der cDNA-Bibliothek und folglich auch auf die Expressionsbibliothek nimmt. Angesichts eines Ungleichgewichts zwischen der Gesamtzahl protein-kodierender Gene in S. Enteritidis zu möglichen Klonen, die während des Microarray-Screenings untersucht werden können, besteht der Bedarf einer Anreicherung von Proteinen in der Expressionsbibliothek. Das infektionsähnliche Modell zeigte, dass nicht nur Virulenz-assoziierte, sondern auch Stress- und Metabolismus-relevante Gene hochreguliert werden. Durch die Konstruktion dieser spezifischen cDNA-Bibliotheken ist die Erkennung von charakteristischen molekularen Markern gegeben. Weiterhin wurden anhand der Transkriptomanalyse spezifisch hochregulierte Gene identifiziert, die relevant für das intrazelluläre Überleben von S. Enteritidis in humanen Epithelzellen sind. Hiervon wurden drei Gene näher untersucht, indem ihr Einfluss im infektionsähnlichen Modell mittels entsprechender Gen-Knockout-Stämme analysiert wurde. Dabei wurde für eine dieser Mutanten ein reduziertes Wachstum in der späten intrazellulären Phase nachgewiesen. Weiterführende in vitro Analysen sind für die Charakterisierung des Knockout-Stamms notwendig, um den Einsatz als potenzielles Therapeutikum zu verifizieren. Zusammenfassend wurde ein in vitro Infektionsmodell für S. Enteritidis etabliert, wodurch neue Zielstrukturen des Erregers identifiziert wurden. Diese sind für diagnostische oder therapeutische Anwendungen interessant. Das Modell lässt sich ebenso für andere intrazelluläre Pathogene übertragen und gewährleistet eine zuverlässige Identifizierung von potentiellen Antigenen.

Gesundheitsmonitoring im Langstreckenmotorsport (2018)

Stoll, Josefine

Professionelle GT Langstreckenmotorsportler (Rennfahrer) müssen den hohen motorischen und kognitiven Ansprüchen ohne Verlust der Performance während eines Rennens endgegenwirken können. Sie müssen stets, bei hoher Geschwindigkeit fokussiert und konzentriert auf ihr Auto, die Rennstrecke und ihre Gegner reagieren können. Darüber hinaus sind Rennfahrer zusätzlich durch die notwendige Kommunikation im Auto mit den Ingenieuren und Mechanikern in der Boxengasse gefordert. Daten über die tatsächliche Beanspruchung und häufig auftretende Beschwerden und/oder Verletzung von Profiathleten liegen kaum vor. Für eine möglichst gute Performance im Auto während eines Rennens ist es notwendige neben der körperlichen Beanspruchung auch die häufigen Krankheitsbilder zu kennen. Auf Basis dessen kann eine optimale Prävention oder notwendige Therapie zur möglichst schnellen Reintegration in den Sport abgeleitet und entwickelt werden. Die vorliegende Arbeit befasst sich durch ein regelmäßiges Gesundheitsmonitoring mit der Erfassung häufiger Beschwerden und oder Verletzungen im GT Langestreckenmotorsport zur Ableitung eines präventiven (trainingstherapeutischen) und therapeutischen Konzeptes. Darüber hinaus, soll über die Einschätzung der körperlichen Leistungsfähigkeit der Athleten, auf Basis der Beanspruchung im Rennfahrzeug ein mögliches Trainingskonzept in Abhängigkeit der Saison entwickelt werden. Insgesamt wurden über 15 Jahre (2003-2017) 37 männliche Athleten aus dem GT Langstreckenmotorsport 353mal im Rahmen eines Gesundheitsmonitorings untersucht. Dabei wurden Athleten maximal 14 Jahre und mindestens 1 Jahr sportmedizinische betreut. Diese 2x im Jahr stattfindende Untersuchung beinhaltete im Wesentlichen eine sportmedizinische Untersuchung zur Einschätzung der Tauglichkeit für den Sport und die Erfassung der körperlichen Leistungsfähigkeit. Über das Gesundheitsmonitoring hinaus erfolgte die Betreuung zusätzlich an der Rennstrecke zur weiteren Erfassung der Beschwerden, Erkrankungen und Verletzungen der Athleten während ihrer sportartspezifischen Belastung. Zusammengefasst zeigen die Athleten geringe Prävalenzen und Inzidenzen der Krankheitsbilder bzw. Beschwerden. Ein Unterschied der Prävalenzen zeigt sich zwischen den Gesundheitsuntersuchungen und der Betreuung an der Rennstrecke. Die häufigsten Beschwerdebilder zeigen sich aus Orthopädie und Innerer Medizin. So sind Infekte der oberen Atemwege sowie Allergien neben Beschwerden der unteren Extremität und der Wirbelsäule am häufigsten. Demzufolge werden vorrangig physio- und trainingstherapeutische Konsequenzen abgeleitet. Eine medikamentöse Therapie erfolgt im Wesentlichen während der Rennbetreuung. Zur Reduktion der orthopädischen und internistischen Beschwerden sollten präventive Maßnahmen mehr betont werden. Die körperliche Leistungsfähigkeit zeigt im Wesentlichen über die Untersuchungsjahre eine stabile Performance für die Ausdauer-, Kraft und sensomotorische Leistungsfähigkeit. Die Ausdauerleistungsfähigkeit kann in Abhängigkeit der Sportartspezifik mit einer guten bis sehr guten Ausprägung definiert werden. Die Kraftleistungsfähigkeit und die sensomotorische Leistungsfähigkeit lassen sportartspezifische Unterschiede zu und sollte körpergewichtsbezogen betrachtet werden. Ein sportmedizinisches und trainingstherapeutisches Konzept müsste demnach eine regelmäßige ärztlich-medizinische Untersuchung mit dem Fokus der Orthopädie, Inneren Medizin und Hals- Nasen-Ohren-Kunde beinhalten. Darüber hinaus sollte eine regelmäßige Erfassung der körperlichen Leistungsfähigkeit zur möglichst effektiven Ableitung von Trainingsinhalten oder Präventionsmaßnahmen berücksichtig werden. Auf Grundlage der hohen Reisetätigkeit und der ganzjährigen Saison könnte ein 1-2x jährlich stattfindendes Trainingslager, im Sinne eines Grundlagen- und Aufbautrainings zur Optimierung der Leistungsfähigkeit beitragen, das Konzept komplementieren. Zudem scheint eine ärztliche Rennbetreuung notwendig.

Augmented liver inflammation in a microsomal prostaglandin E synthase 1 (mPGES-1)-deficient diet-induced mouse NASH model (2018)

Henkel, Janin ; Coleman, Charles Dominic ; Schraplau, Anne ; Jöhrens, Korinna ; Weiss, Thomas Siegfried ; Jonas, Wenke ; Schürmann, Annette ; Püschel, Gerhard Paul

In a subset of patients, non-alcoholic fatty liver disease (NAFLD) is complicated by cell death and inflammation resulting in non-alcoholic steatohepatitis (NASH), which may progress to fibrosis and subsequent organ failure. Apart from cytokines, prostaglandins, in particular prostaglandin E-2 (PGE(2)), play a pivotal role during inflammatory processes. Expression of the key enzymes of PGE(2) synthesis, cyclooxygenase 2 and microsomal PGE synthase 1 (mPGES-1), was increased in human NASH livers in comparison to controls and correlated with the NASH activity score. Both enzymes were also induced in NASH-diet-fed wild-type mice, resulting in an increase in hepatic PGE(2) concentration that was completely abrogated in mPGES-1-deficient mice. PGE(2) is known to inhibit TNF-alpha synthesis in macrophages. A strong infiltration of monocyte-derived macrophages was observed in NASH-diet-fed mice, which was accompanied with an increase in hepatic TNF-alpha expression. Due to the impaired PGE(2) production, TNF-alpha expression increased much more in livers of mPGES-1-deficient mice or in the peritoneal macrophages of these mice. The increased levels of TNF-alpha resulted in an enhanced IL-1 beta production, primarily in hepatocytes, and augmented hepatocyte apoptosis. In conclusion, attenuation of PGE(2) production by mPGES-1 ablation enhanced the TNF-alpha-triggered inflammatory response and hepatocyte apoptosis in diet-induced NASH.

Augmented liver inflammation in a microsomal prostaglandin E synthase 1 (mPGES-1)-deficient diet-induced mouse NASH model (2018)

Henkel, Janin ; Coleman, Charles Dominic ; Schraplau, Anne ; Jöhrens, Korinna ; Weiss, Thomas Siegfried ; Jonas, Wenke ; Schürmann, Annette ; Püschel, Gerhard Paul

In a subset of patients, non-alcoholic fatty liver disease (NAFLD) is complicated by cell death and inflammation resulting in non-alcoholic steatohepatitis (NASH), which may progress to fibrosis and subsequent organ failure. Apart from cytokines, prostaglandins, in particular prostaglandin E-2 (PGE(2)), play a pivotal role during inflammatory processes. Expression of the key enzymes of PGE(2) synthesis, cyclooxygenase 2 and microsomal PGE synthase 1 (mPGES-1), was increased in human NASH livers in comparison to controls and correlated with the NASH activity score. Both enzymes were also induced in NASH-diet-fed wild-type mice, resulting in an increase in hepatic PGE(2) concentration that was completely abrogated in mPGES-1-deficient mice. PGE(2) is known to inhibit TNF-alpha synthesis in macrophages. A strong infiltration of monocyte-derived macrophages was observed in NASH-diet-fed mice, which was accompanied with an increase in hepatic TNF-alpha expression. Due to the impaired PGE(2) production, TNF-alpha expression increased much more in livers of mPGES-1-deficient mice or in the peritoneal macrophages of these mice. The increased levels of TNF-alpha resulted in an enhanced IL-1 beta production, primarily in hepatocytes, and augmented hepatocyte apoptosis. In conclusion, attenuation of PGE(2) production by mPGES-1 ablation enhanced the TNF-alpha-triggered inflammatory response and hepatocyte apoptosis in diet-induced NASH.

Landscape evolution in the western Indian Himalaya since the Miocene (2018)

Eugster, Patricia

The Himalayan arc stretches >2500 km from east to west at the southern edge of the Tibetan Plateau, representing one of the most important Cenozoic continent-continent collisional orogens. Internal deformation processes and climatic factors, which drive weathering, denudation, and transport, influence the growth and erosion of the orogen. During glacial times wet-based glaciers sculpted the mountain range and left overdeepend and U-shaped valleys, which were backfilled during interglacial times with paraglacial sediments over several cycles. These sediments partially still remain within the valleys because of insufficient evacuation capabilities into the foreland. Climatic processes overlay long-term tectonic processes responsible for uplift and exhumation caused by convergence. Possible processes accommodating convergence within the orogenic wedge along the main Himalayan faults, which divide the range into four major lithologic units, are debated. In this context, the identification of processes shaping the Earth’s surface on short- and on long-term are crucial to understand the growth of the orogen and implications for landscape development in various sectors along the arc. This thesis focuses on both surface and tectonic processes that shape the landscape in the western Indian Himalaya since late Miocene. In my first study, I dated well-preserved glacially polished bedrock on high-elevated ridges and valley walls in the upper of the Chandra Valley the by means of 10Be terrestrial cosmogenic radionuclides (TCN). I used these ages and mapped glacial features to reconstruct the extent and timing of Pleistocene glaciation at the southern front of the Himalaya. I was able to reconstruct an extensive valley glacier of ~200 km length and >1000 m thickness. Deglaciation of the Chandra Valley glacier started subsequently to insolation increase on the Northern Hemisphere and thus responded to temperature increase. I showed that the timing this deglaciation onset was coeval with retreat of further midlatitude glaciers on the Northern and Southern Hemispheres. These comparisons also showed that the post-LGM deglaciation very rapid, occurred within a few thousand years, and was nearly finished prior to the Bølling/Allerød interstadial. A second study (co-authorship) investigates how glacial advances and retreats in high mountain environments impact the landscape. By 10Be TCN dating and geomorphic mapping, we obtained maximal length and height of the Siachen Glacier within the Nubra Valley. Today the Shyok and Nubra confluence is backfilled with sedimentary deposits, which are attributed to the valley blocking of the Siachen Glacier 900 m above the present day river level. A glacial dam of the Siachen Glacier blocked the Shyok River and lead to the evolution of a more than 20 km long lake. Fluvial and lacustrine deposits in the valley document alternating draining and filling cycles of the lake dammed by the Siachen Glacier. In this study, we can show that glacial incision was outpacing fluvial incision. In the third study, which spans the million-year timescale, I focus on exhumation and erosion within the Chandra and Beas valleys. In this study the position and discussed possible reasons of rapidly exhuming rocks, several 100-km away from one of the main Himalayan faults (MFT) using Apatite Fission Track (AFT) thermochronometry. The newly gained AFT ages indicate rapid exhumation and confirm earlier studies in the Chandra Valley. I assume that the rapid exhumation is most likely related to uplift over subsurface structures. I tested this hypothesis by combining further low-temperature thermochronometers from areas east and west of my study area. By comparing two transects, each parallel to the Beas/Chandra Valley transect, I demonstrate similarities in the exhumation pattern to transects across the Sutlej region, and strong dissimilarities in the transect crossing the Dhauladar Range. I conclude that the belt of rapid exhumation terminates at the western end of the Kullu-Rampur window. Therewith, I corroborate earlier studies suggesting changes in exhumation behavior in the western Himalaya. Furthermore, I discussed several causes responsible for the pronounced change in exhumation patterns along strike: 1) the role of inherited pre-collisional features such as the Proterozoic sedimentary cover of the Indian basement, former ridges and geological structures, and 2) the variability of convergence rates along the Himalayan arc due to an increased oblique component towards the syntaxis. The combination of field observations (geological and geomorphological mapping) and methods to constrain short- and long-term processes (10Be, AFT) help to understand the role of the individual contributors to exhumation and erosion in the western Indian Himalaya. With the results of this thesis, I emphasize the importance of glacial and tectonic processes in shaping the landscape by driving exhumation and erosion in the studied areas.

Algebraic foundation of Group Field Theory (2018)

Kegeles, Alexander

In this thesis we provide a construction of the operator framework starting from the functional formulation of group field theory (GFT). We define operator algebras on Hilbert spaces whose expectation values in specific states provide correlation functions of the functional formulation. Our construction allows us to give a direct relation between the ingredients of the functional GFT and its operator formulation in a perturbative regime. Using this construction we provide an example of GFT states that can not be formulated as states in a Fock space and lead to math- ematically inequivalent representations of the operator algebra. We show that such inequivalent representations can be grouped together by their symmetry properties and sometimes break the left translation symmetry of the GFT action. We interpret these groups of inequivalent representations as phases of GFT, similar to the classification of phases that we use in QFT’s on space-time.

Was bewirken Gebietsreformen? (2018)

Kuhlmann, Sabine ; Seyfried, Markus ; Siegel, John

Der vorliegende Beitrag fasst die bisherige Forschung über die Wirkungen von Gebietsreformen zusammen und analysiert diese aus inhaltlicher und methodischer Perspektive. Basierend auf einer Auswertung von ausgewählten nationalen und europäischen Studien werden Wirkungsbefunde in drei zentralen Dimensionen dargestellt: (1) Leistungsfähigkeit, Verwaltungs- und Veranstaltungskraft, (2) Einsparungen, Skalenerträge und Wirtschaftlichkeit und (3) Partizipation und demokratische Kontrolle. Im Ergebnis kann festgestellt werden, dass die Leistungs- und Handlungsfähigkeit kommunaler Verwaltungen durch Gebietsreformen überwiegend positiv beeinflusst wird. Dagegen sind die empirischen Befunde bezüglich Wirtschaftlichkeit, Einsparungen und Skalenerträge sowie zur Partizipation und demokratischen Kontrolle nicht eindeutig.

E-Learning Symposium 2018 (2018)

Keil, Reinhard ; Konert, Johannes ; Damnik, Gregor ; Gierl, Mark J. ; Proske, Antje ; Körndle, Hermann ; Narciss, Susanne ; Wahl, Marina ; Hölscher, Michael ; Mariani, Ennio ; Jaisli, Isabel ; Tscherejkina, Anna ; Morgiel, Anna ; Moebert, Tobias ; Herbstreit, Stephanie ; Mäker, Daniela ; Szalai, Cynthia ; Braun, Iris ; Kapp, Felix ; Hara, Tenshi C. ; Kubica, Tommy ; Stumpf, Sarah

In den vergangenen Jahren sind viele E-Learning-Innovationen entstanden. Einige davon wurden auf den vergangenen E-Learning Symposien der Universität Potsdam prä¬sentiert: Das erste E-Learning Symposium im Jahr 2012 konzentrierte sich auf unter-schiedliche Möglichkeiten der Studierendenaktivierung und Lehrgestaltung. Das Sym¬posium 2014 rückte vor allem die Studierenden ins Zentrum der Aufmerksamkeit. Im Jahr 2016 kam es durch das Zusammengehen des Symposiums mit der DeLFI-Tagung zu einer Fokussierung auf technische Innovationen. Doch was ist aus den Leucht¬türmen von gestern geworden, und brauchen wir überhaupt noch neue Leuchttürme? Das Symposium setzt sich in diesem Jahr unter dem Motto „Innovation und Nachhaltigkeit – (k)ein Gegensatz?“ mit mediengestützten Lehr- und Lernprozessen im universitären Kontext auseinander und reflektiert aktuelle technische sowie didaktische Ent¬wick¬lungen mit Blick auf deren mittel- bis langfristigen Einsatz in der Praxis. Dieser Tagungsband zum E-Learning Symposium 2018 an der Universität Potsdam beinhaltet eine Mischung von Forschungs- und Praxisbeiträgen aus verschiedenen Fach-disziplinen und eröffnet vielschichtige Perspektiven auf das Thema E-Learning. Dabei werden die Vielfalt der didaktischen Einsatzszenarien als auch die Potentiale von Werk-zeugen und Methoden der Informatik in ihrem Zusammenspiel beleuchtet. In seiner Keynote widmet sich Reinhard Keil dem Motto des Symposiums und geht der Nachhaltigkeit bei E-Learning-Projekten auf den Grund. Dabei analysiert und beleuchtet er anhand seiner über 15-jährigen Forschungspraxis die wichtigsten Wirkfaktoren und formuliert Empfehlungen zur Konzeption von E-Learning-Projekten. Im Gegensatz zu rein auf Kostenersparnis ausgerichteten (hochschul-)politischen Forderungen pro-klamiert er den Ansatz der hypothesengeleiteten Technikgestaltung, in der Nachhaltig-keit als Leitfrage oder Forschungsstrategie verstanden werden kann. In eine ähnliche Richtung geht der Beitrag von Iris Braun et al., die über Erfolgsfaktoren beim Einsatz von Audience Response Systemen in der universitären Lehre berichten. Ein weiteres aktuelles Thema, sowohl für die Bildungstechnologie als auch in den Bildungswissenschaften allgemein, ist die Kompetenzorientierung und –modellierung. Hier geht es darum (Problemlöse-)Fähigkeiten gezielt zu beschreiben und in den Mittel-punkt der Lehre zu stellen. Johannes Konert stellt in einem eingeladenen Vortrag zwei Projekte vor, die den Prozess beginnend bei der Definition von Kompetenzen, deren Modellie¬rung in einem semantischen maschinenlesbaren Format bis hin zur Erarbeitung von Methoden zur Kompetenzmessung und der elektronischen Zertifizie¬rung aufzeigen. Dabei geht er auf technische Möglichkeiten, aber auch Grenzen ein. Auf einer spezifi¬scheren Ebene beschäftigt sich Sarah Stumpf mit digitalen bzw. medien¬didak¬tischen Kompetenzen im Lehramtsstudium und stellt ein Framework für die Förderung ebensol¬cher Kompetenzen bei angehenden Lehrkräften vor. Der Einsatz von E-Learning birgt noch einige Herausforderungen. Dabei geht es oft um die Verbindung von Didaktik und Technik, den Erhalt von Aufmerksamkeit oder den Aufwand für das Erstellen von interaktiven Lehr- und Lerninhalten. Drei Beiträge in diesem Tagungsband beschäftigen sich mit dieser Thematik in unterschiedlichen Kon-texten und zeigen Best-Practices und Lösungsansätze auf: Der Beitrag von Martina Wahl und Michael Hölscher behandelt den besonderen Kontext von Blended Learning-Szenarien in berufsbegleitenden Studiengängen. Um die Veröffentlichung eines global frei verfügbaren Onlinekurses abseits der großen MOOC Plattformen und den didak-tischen Herausforderungen auch hinsichtlich der Motivation geht es im Beitrag von Ennio Marani und Isabel Jaisli. Schließlich schlagen Gregor Damnik et al. die auto¬matische Erzeugung von Aufgaben zur Erhöhung von Interaktivität und Adaptivität in digitalen Lernressourcen vor, um den teilweise erheblichen Erstellungsaufwand zu redu¬zieren. Zum Thema E-Learning zählen auch immer mobile Apps bzw. Spiele. Gleich zwei Beiträge beschäftigen sich mit dem Einsatz von E-Learning-Tools im Gesundheits-kontext: Anna Tscherejkina und Anna Morgiel stellen in ihrem Beitrag Minispiele zum Training von sozio-emotionalen Kompetenzen für Menschen mit Autismus vor, und Stephanie Herbstreit et al. berichten vom Einsatz einer mobilen Lern-App zur Ver¬besserung von klinisch-praktischem Unterricht.

Moving arms (2018)

Werner, Karsten ; Raab, Markus ; Fischer, Martin H.

Embodied cognition postulates a bi-directional link between the human body and its cognitive functions. Whether this holds for higher cognitive functions such as problem solving is unknown. We predicted that arm movement manipulations performed by the participants could affect the problem-solving solutions. We tested this prediction in quantitative reasoning tasks that allowed two solutions to each problem (addition or subtraction). In two studies with healthy adults (N=53 and N=50), we found an effect of problem-congruent movements on problem solutions. Consistent with embodied cognition, sensorimotor information gained via right or left arm movements affects the solution in different types of problem-solving tasks.

Kommunale Kinder- und Jugendgremien im Land Brandenburg (2018)

Rollfing, Petra

Kommunale Kinder- und Jugendgremien sind eine Möglichkeit, junge Menschen in der Kommune zu beteiligen. Die Masterarbeit beschäftigt sich mit den kommunalen Kinder- und Jugendgremien im Land Brandenburg, über die es in der wissenschaftlichen Literatur nur wenige Erkenntnisse gibt. Die Arbeit gibt einen Überblick über die bestehenden Gremien und zeigt, welche Kinder und Jugendliche sich beteiligen und wie der Entwicklungsstand der Gremien ist. Ausgehend von der Partizipationsleiter von Hart geht die Arbeit zudem in zwei Fallstudien in Senftenberg und Oranienburg der Frage nach, ob es sich bei den Kinder- und Jugendgremien um ernstgemeinte Partizipation handelt.

Vom Kadetten bis zum Admiral (2018)

Jentzsch, Christian

Die Royal Navy und die Kaiserliche Marine erlebten seit dem letzten Drittel des 19. Jahrhunderts einen grundlegenden Wandel. Prägten anfangs Segelschiffhybride als Hauptkampfmittel die beiden Flotten, dominierten sie Großkampfschiffe vor Beginn des Ersten Weltkrieges. Aber auch U-Boote und Seeflugzeuge spielten eine zunehmende Rolle. Vor allem die drastischen technologischen und gesellschaftlichen Entwicklungen wirkten sich auf die Aufgaben, Rekrutierung und Ausbildung der Offiziere aus. Christian Jentzsch untersucht, wie das Seeoffizierkorps der Kaiserlichen Marine und das Executive Officer Corps der Royal Navy auf die Herausforderungen reagierten.

Psychometric properties of figure rating scales in children (2018)

Zitzmann, Jana ; Warschburger, Petra

This study examined psychometric properties of figure rating scales, particularly the effects of ascending silhouette ordering, in 153 children, 9 to 13 years old. Two versions of Collins’s (1991) figural rating scale were presented: the original scale (figures arranged ascendingly) and a modified version (randomized figure ordering. Ratings of current and ideal figure were elicited and body dissatisfaction was calculated. All children were randomly assigned to one of two subgroups and completed both scale versions in a different sequence. There were no significant differences in figure selection and body dissatisfaction between the two figure orderings. Regarding the selection of the current figure, results showed that girls are more affected by the silhouette ordering than boys. Our results suggest that figure rating scales are both valid and reliable, whereby correlation coefficients reveal greater stability for ideal figure selections and body dissatisfaction ratings when using the scale with ascending figure ordering.

The Requisite Rigour in the Identification of Customary International Law (2018)

Arajärvi, Noora

Over the last few decades, the methodology for the identification of customary international law (CIL) has been changing. Both elements of CIL – practice and opinio juris – have assumed novel and broader forms, as noted in the Reports of the Special Rapporteur of the International Law Commission (2013, 2014, 2015, 2016). This paper discusses these Reports and the draft conclusions, and reaction by States in the Sixth Committee of the United Nations General Assembly (UNGA), highlighting the areas of consensus and contestation. This ties to the analysis of the main doctrinal positions, with special attention being given to the two elements of CIL, and the role of the UNGA resolutions. The underlying motivation is to assess the real or perceived crisis of CIL, and the author develops the broader argument maintaining that in order to retain unity within international law, the internal limits of CIL must be carefully asserted.

Forging an Italian hero? (2018)

Scianna, Bastian Matteo

Over the last two decades, Amedeo Guillet (1909–2010) has been turned into a public and military hero. His exploits as a guerrilla leader in Italian East Africa in 1941 have been exaggerated to forge a narrative of an honourable resistance against overwhelming odds. Thereby, Guillet has been showcased as a romanticized colonial explorer who was an apolitical and timeless Italian officer. He has been compared to Lawrence of Arabia in order to raise his international visibility, while his genuine Italian brand is perpetuated domestically. By elevating him to an official role model, the Italian Army has gained a focal point for military heroism that was also acceptable in the public memory as the embodiment of a ‘glorious’ defeat narrative.

Straftaten in virtuellen Welten (2017)

Bosch, Sebastian

Virtuelle Welten wie World of Warcraft oder Second Life werden weltweit von vielen Millionen Menschen genutzt. Aufgrund der vielfältigen Interaktionsmöglichkeiten der Nutzer untereinander kommt es dabei immer wieder auch zu Verhaltensweisen, die die Frage nach strafrechtlicher Relevanz aufwerfen. Sebastian Bosch greift in seiner Arbeit einzelne Tatbestände des deutschen StGB heraus und untersucht diese auf ihre Begehbarkeit innerhalb virtueller Welten. Besondere Aufmerksamkeit wird dabei dem Betrug gem. § 263 StGB gewidmet, da dieser im Hinblick auf den speziellen »Tatort« der virtuellen Welten besonders interessante Fragen aufwirft. Im Anschluss daran wird untersucht, ob der Täter einer solchen Straftat auch entsprechend bestraft werden sollte, oder ob sich aufgrund des Spielcharakters virtueller Welten nicht eine Privilegierung aufdrängt, wie sie aus dem Sportstrafrecht bekannt ist. Der Autor gelangt zu dem Ergebnis, dass eine Privilegierung vorgenommen werden sollte, soweit es sich bei der verletzenden Handlung um eine spiel- bzw. weltenkonforme Handlung handelt.

Weiterhin vergessen: Der Krieg in Jemen (2018)

Schmidt, Renate

Testing the Consensus Model (2018)

Liepertz, Sven ; Borowski, Andreas

The structure and definition of professional knowledge is a continuing focus of science education research. In 2012, a pedagogical content knowledge (PCK) summit was held and it suggested a model of professional knowledge and skill including PCK, which was later often called the Consensus Model (Gess-Newsome, 2015. A model of teacher professional knowledge and skill including PCK: Results of the thinking from the PCK summit. In A. Berry, P. J. Friedrichsen, & J. Loughran (Eds.), Teaching and learning in science series. Re-examining pedagogical content knowledge in science education (1st ed., pp. 28–42). New York, NY: Routledge). The Consensus Model proposes a potential powerful framework for the relations among teachers’ different professional knowledge bases, but to date it has neither been investigated empirically nor systematically. In this study, we investigated the relationships suggested by the Consensus Model among different aspects of teachers’ knowledge and skill. A sample of 35 physics teachers and their classes participated in the investigation; both teachers and their students in these classes took paper-and-pencil tests. Furthermore, a lesson taught by each of the teachers was videotaped and analysed. The video analysis focused on the interconnectedness of the content structure of the lesson as representation of the in-class actions of the teachers. The interconnectedness is understood as a direct result of the application of professional knowledge of the teachers to their teaching. The teachers’ knowledge showed no significant influence on the interconnectedness of the lesson content structure. However, the results confirmed the influence of interconnectedness and certain aspects of professional knowledge on students’ outcomes. Therefore, interconnectedness of content structure could be verified as one indicator of teachers’ instructional quality.

The complete mitochondrial genome of the common vole, Microtus arvalis (Rodentia: Arvicolinae) (2018)

Folkertsma, Remco ; Westbury, Michael V. ; Eccard, Jana Anja ; Hofreiter, Michael

The common vole, Microtus arvalis belongs to the genus Microtus in the subfamily Arvicolinae. In this study, the complete mitochondrial genome of M. arvalis was recovered using shotgun sequencing and an iterative mapping approach using three related species. Phylogenetic analyses using the sequence of 21 arvicoline species place the common vole as a sister species to the East European vole (Microtus levis), but as opposed to previous results we find no support for the recognition of the genus Neodon within the subfamily Arvicolinae, as this is, as well as the genus Lasiopodomys, found within the Microtus genus.

Selective effects of age of acquisition on morphological priming (2017)

Veríssimo, João ; Heyer, Vera ; Jacob, Gunnar ; Clahsen, Harald

Is there an ideal time window for language acquisition after which nativelike representation and processing are unattainable? Although this question has been heavily debated, no consensus has been reached. Here, we present evidence for a sensitive period in language development and show that it is specific to grammar. We conducted a masked priming task with a group of Turkish-German bilinguals and examined age of acquisition (AoA) effects on the processing of complex words. We compared a subtle but meaningful linguistic contrast, that between grammatical inflection and lexical-based derivation. The results showed a highly selective AoA effect on inflectional (but not derivational) priming. In addition, the effect displayed a discontinuity indicative of a sensitive period: Priming from inflected forms was nativelike when acquisition started before the age of 5 but declined with increasing AoA. We conclude that the acquisition of morphological rules expressing morphosyntactic properties is constrained by maturational factors.

Europeanisation, stateness, and professions (2017)

Schmidt-Wellenburg, Christian

The article explores Europeanisation as an effect of European political integration, a process driven by struggles over the legitimate political and social order that is to prevail in Europe. Firstly, an analytic framework is constructed, drawing on insights from Pierre Bourdieu’s work on similar struggles over nation-stateness. Secondly, the mechanisms identified are used to assess the role played by economic experts and expertise in the process of European political integration. It is argued that concepts arising from economic disciplines, agents educated in economics, and practising economic professionals influence European political integration and have benefited from Europeanisation initiated by this process. Special emphasis is placed on strategies of integrating Europe by law or by market, on governing Europe using economic expertise, on the role played by economic academia in researching and objectifying Europe, and on staffing European institutions with economists.

Rights and Obligations of Third Parties in Armed Conflict (2016)

Krieger, Heike

This paper will turn into a contribution to a book on community obligations. It focusses on third parties' rights and obligations in armed conflict. It is often said that international law has developed from a legal order which is designed to protect sovereignty to a system which also promotes community interests. This shift is said to be reflected in structural changes of the legal system. The creation of rights and obligations for third parties is generally seen as a part of this perceived paradigmatic shift. Community interests can be furthered either by negative duties of abstention, by an entitlement for third states, or even by duties to take positive measures. Since the shift towards protecting community interests apparently requires some form of cooperation, positive rights and duties to protect and to promote appear to be indispensable. Authors relying on a community perspective often dismiss duties of abstention as an expression of indifference in the face of a violation of a fundamental norm. Solidarity seems to require that third states take a more proactive role in actively enforcing community interests. The paper aims to test this understanding on the basis of an analysis of rights and obligations of third states in armed conflict. In order to argue that duties of abstention of third states are a central instrument for promoting community interests in relation to armed conflicts, the paper will first trace pertinent structural changes in international law. In particular, it will question the extent to which positive rights and obligations of third states have been firmly established in international law. In a second step, this contribution will evaluate the overall tendencies in the ongoing lawmaking process for promoting community interests in relation to armed conflict.

Biomarker Discovery in Multiple Sclerosis and Parkinson’s disease (2018)

Stößel, Daniel

Neuroinflammatory and neurodegenerative diseases such as Parkinson's (PD) and multiple sclerosis (MS) often result in a severe impairment of the patient´s quality of life. Effective therapies for the treatment are currently not available, which results in a high socio-economic burden. Due to the heterogeneity of the disease subtypes, stratification is particularly difficult in the early phase of the disease and is mainly based on clinical parameters such as neurophysiological tests and central nervous imaging. Due to good accessibility and stability, blood and cerebrospinal fluid metabolite markers could serve as surrogates for neurodegenerative processes. This can lead to an improved mechanistic understanding of these diseases and further be used as "treatment response" biomarkers in preclinical and clinical development programs. Therefore, plasma and CSF metabolite profiles will be identified that allow differentiation of PD from healthy controls, association of PD with dementia (PDD) and differentiation of PD subtypes such as akinetic rigid and tremor dominant PD patients. In addition, plasma metabolites for the diagnosis of primary progressive MS (PPMS) should be investigated and tested for their specificity to relapsing-remitting MS (RRMS) and their development during PPMS progression. By applying untargeted high-resolution metabolomics of PD patient samples and in using random forest and partial least square machine learning algorithms, this study identified 20 plasma metabolites and 14 CSF metabolite biomarkers. These differentiate against healthy individuals with an AUC of 0.8 and 0.9 in PD, respectively. We also identify ten PDD specific serum metabolites, which differentiate against healthy individuals and PD patients without dementia with an AUC of 1.0, respectively. Furthermore, 23 akinetic-rigid specific plasma markers were identified, which differentiate against tremor-dominant PD patients with an AUC of 0.94 and against healthy individuals with an AUC of 0.98. These findings also suggest more severe disease pathology in the akinetic-rigid PD than in tremor dominant PD. In the analysis of MS patient samples a partial least square analysis yielded predictive models for the classification of PPMS and resulted in 20 PPMS specific metabolites. In another MS study unknown changes in human metabolism were identified after administration of the multiple sclerosis drug dimethylfumarate, which is used for the treatment of RRMS. These results allow to describe and understand the hitherto completely unknown mechanism of action of this new drug and to use these findings for the further development of new drugs and targets against RRMS. In conclusion, these results have the potential for improved diagnosis of these diseases and improvement of mechanistic understandings, as multiple deregulated pathways were identified. Moreover, novel Dimethylfumarate targets can be used to aid drug development and treatment efficiency. Overall, metabolite profiling in combination with machine learning identified as a promising approach for biomarker discovery and mode of action elucidation.

Der "immerwährende Staatssekretär" (2019)

Apostolow, Markus

Als erster Staatssekretär des 1949 gegründeten Bundesministeriums der Justiz war Walter Strauß maßgeblich für dessen personellen Aufbau verantwortlich. Während seiner Amtszeit, die erst 1963 endete, diente Strauß unter fünf verschiedenen Ministern. Damit verkörperte er die Kontinuität der Arbeit und galt nicht von ungefähr als der eigentliche 'Herrscher der Rosenburg', dem Bonner Amtssitz des Ministeriums. Durch seinen Führungsstil, der die Forderung nach Qualität mit einem geradezu paternalistischen Verantwortungsgefühl verband, prägte der Gründungsstaatssekretär den Geist des Hauses für lange Zeit. Obwohl er jüdischer Herkunft war und im Nationalsozialismus zum Kreis der rassisch Verfolgten gehört hatte, griff Strauß bei der Auswahl des Personals allerdings in hohem Maße auf die Mitarbeit von Personen zurück, die durch ihre Tätigkeit im 'Dritten Reich' belastet waren. Warum dies so war, sucht der Autor nicht nur anhand biografischer Prägungen, die Strauß im Kaiserreich, in der Weimarer Republik, dem Nationalsozialismus und der Besatzungszeit erfahren hatte, sondern auch durch eine umfassende Darstellung der wesentlichen Merkmale und Kennzeichen seiner Personalpolitik zu ergründen: Wie weit reichte sein Einfluss Welche Rolle spielte er bei Auswahl und Beförderungen, in erster Linie der Beamten des höheren Dienstes, in Abgrenzung zu anderen Akteuren Und in welchem Maße war er bei seinen Entscheidungen durch institutionelle Rahmenbedingungen eingeschränkt 366 pp

Colonial Injustices and the Law of State Responsibility (2017)

Buser, Andreas

Caribbean States organised in CARICOM recently brought forward reparation claims against several European States to compensate slavery and (native) genocides in the Caribbean and even threatened to approach the International Court of Justice. The paper provides for an analysis of the facts behind the CARICOM claim and asks whether the law of state responsibility is able to provide for the demanded compensation. As the intertemporal principle generally prohibits retroactive application of today’s international rules, the paper argues that the complete claim must be based on the law of state responsibility governing in the time of the respective conduct. An inquiry into the history of primary (prohibition of slavery and genocide) as well as secondary rules of State responsibility reveals that both sets of rules were underdeveloped or non-existent at the times of slavery and alleged (native) genocides. Therefore, the author concludes that the CARICOM claim is legally flawed but nevertheless worth the attention as it once again exposes imperial and colonial injustices of the past and their legitimization by historical international law and international/natural lawyers.

Subtle fingers – tangible numbers: The influence of finger counting experience on mental number representations (2018)

Sixtus, Elena

Numbers are omnipresent in daily life. They vary in display format and in their meaning so that it does not seem self-evident that our brains process them more or less easily and flexibly. The present thesis addresses mental number representations in general, and specifically the impact of finger counting on mental number representations. Finger postures that result from finger counting experience are one of many ways to convey numerical information. They are, however, probably the one where the numerical content becomes most tangible. By investigating the role of fingers in adults’ mental number representations the four presented studies also tested the Embodied Cognition hypothesis which predicts that bodily experience (e.g., finger counting) during concept acquisition (e.g., number concepts) stays an immanent part of these concepts. The studies focussed on different aspects of finger counting experience. First, consistency and further details of spontaneously used finger configurations were investigated when participants repeatedly produced finger postures according to specific numbers (Study 1). Furthermore, finger counting postures (Study 2), different finger configurations (Study 2 and 4), finger movements (Study 3), and tactile finger perception (Study 4) were investigated regarding their capability to affect number processing. Results indicated that active production of finger counting postures and single finger movements as well as passive perception of tactile stimulation of specific fingers co-activated associated number knowledge and facilitated responses towards corresponding magnitudes and number symbols. Overall, finger counting experience was reflected in specific effects in mental number processing of adult participants. This indicates that finger counting experience is an immanent part of mental number representations. Findings are discussed in the light of a novel model. The MASC (Model of Analogue and Symbolic Codes) combines and extends two established models of number and magnitude processing. Especially a symbolic motor code is introduced as an essential part of the model. It comprises canonical finger postures (i.e., postures that are habitually used to represent numbers) and finger-number associations. The present findings indicate that finger counting functions both as a sensorimotor magnitude and as a symbolic representational format and that it thereby directly mediates between physical and symbolic size. The implications are relevant both for basic research regarding mental number representations and for pedagogic practices regarding the effectiveness of finger counting as a means to acquire a fundamental grasp of numbers.

Synthesis of artifical building blocks for sortase-mediated ligation and their enzymatic linkage (2018)

Dai, Xiaolin

The enzyme Sortase A catalyzes the formation of a peptide bond between the recognition sequence LPXTG and an oligoglycine. While manifold ligations between proteins and various biomolecules, proteins and small synthetic molecules as well as proteins and surfaces have been reported, the aim of this thesis was to investigate the sortase-catalyzed linkage between artificial building blocks. Hence, this could pave the way for the use of sortase A for tasks from a chemical point of view and maybe even materials science. For the proof of concept, the studied systems were kept as simple as possible at first by choosing easily accessible silica NPs and commercially available polymers. These building blocks were functionalized with peptide motifs for sortase-mediated ligation. Silica nanoparticles were synthesized with diameters of 60 and 200 nm and surface modified with C=C functionalities. Then, peptides bearing a terminal cysteine were covalently linked by means of a thiol-ene reaction. 60 nm SiO2 NPs were functionalized with pentaglycines, while peptides with LPETG motif were linked to 200 nm silica particles. Polyethyleneglycol (PEG) and poly(N isopropylacrylamide) (PNIPAM) were likewise functionalized with peptides by thiol-ene reaction between cysteine residues and C=C units in the polymer end groups. Hence, G5-PEG and PNIPAM-LPETG conjugates were obtained. With this set of building blocks, NP–polymer hybrids, NP–NP, and polymer–polymer structures were generated by sortase-mediated ligation and the product formation shown by transmission electron microscopy, MALDI-ToF mass spectrometry and dynamic light scatting, among others. Thus, the linkage of these artificial building blocks by the enzyme sortase A could be demonstrated. However, when using commercially available polymers, the purification of the polymer–peptide conjugates was impossible and resulted in a mixture containing unmodified polymer. Therefore, strategies were developed for the own synthesis of pure peptide-polymer and polymer-peptide conjugates as building blocks for sortase-mediated ligation. The designed routes are based on preparing polymer blocks via RAFT polymerization from CTAs that are attached to N- or C-terminus, respectively, of a peptide. GG-PNIPAM was synthesized through attachment of a suitable RAFT CTA to Fmoc-GG in an esterification reaction, followed by polymerization of NIPAM and cleavage of the Fmoc protection group. Furthermore, several peptides were synthesized by solid-phase peptide synthesis. The linkage of a RAFT CTA (or polymerization initiator) to the N-terminus of a peptide can be conducted in an automated fashion as last step in a peptide synthesizer. The synthesis of such a conjugate couldn’t be realized in the time frame of this thesis, but many promising strategies exist to continue this strategy using different coupling reagents. Such polymer building blocks can be used to synthesize protein-polymer conjugates catalyzed by sortase A and the approach can be carried on to the synthesis of block copolymers by using polymer blocks with peptide motifs on both ends. Although the proof of concept demonstrated in this thesis only shows examples that can be also synthesized by exclusively chemical techniques, a toolbox of such building blocks will enable the future formation of new materials and pave the way for the application of enzymes in materials science. In addition to nanoparticle systems and block copolymers, this also includes combination with protein-based building blocks to form hybrid materials. Hence, sortase could become an enzymatic tool that complements established chemical linking technologies and provides specific peptide motifs that are orthogonal to all existing chemical functional groups.

Symbolic regression for identification, prediction, and control of dynamical systems (2018)

Quade, Markus

In the present work, we use symbolic regression for automated modeling of dynamical systems. Symbolic regression is a powerful and general method suitable for data-driven identification of mathematical expressions. In particular, the structure and parameters of those expressions are identified simultaneously. We consider two main variants of symbolic regression: sparse regression-based and genetic programming-based symbolic regression. Both are applied to identification, prediction and control of dynamical systems. We introduce a new methodology for the data-driven identification of nonlinear dynamics for systems undergoing abrupt changes. Building on a sparse regression algorithm derived earlier, the model after the change is defined as a minimum update with respect to a reference model of the system identified prior to the change. The technique is successfully exemplified on the chaotic Lorenz system and the van der Pol oscillator. Issues such as computational complexity, robustness against noise and requirements with respect to data volume are investigated. We show how symbolic regression can be used for time series prediction. Again, issues such as robustness against noise and convergence rate are investigated us- ing the harmonic oscillator as a toy problem. In combination with embedding, we demonstrate the prediction of a propagating front in coupled FitzHugh-Nagumo oscillators. Additionally, we show how we can enhance numerical weather predictions to commercially forecast power production of green energy power plants. We employ symbolic regression for synchronization control in coupled van der Pol oscillators. Different coupling topologies are investigated. We address issues such as plausibility and stability of the control laws found. The toolkit has been made open source and is used in turbulence control applications. Genetic programming based symbolic regression is very versatile and can be adapted to many optimization problems. The heuristic-based algorithm allows for cost efficient optimization of complex tasks. We emphasize the ability of symbolic regression to yield white-box models. In contrast to black-box models, such models are accessible and interpretable which allows the usage of established tool chains.

Combination of Agility and Plyometric Training Provides Similar Training Benefits as Combined Balance and Plyometric Training in Young Soccer Players (2018)

Makhlouf, Issam ; Chaouachi, Anis ; Chaouachi, Mehdi ; Othman, Aymen Ben ; Granacher, Urs

Introduction: Studies that combined balance and resistance training induced larger performance improvements compared with single mode training. Agility exercises contain more dynamic and sport-specific movements compared with balance training. Thus, the purpose of this study was to contrast the effects of combined balance and plyometric training with combined agility and plyometric training and an active control on physical fitness in youth. Methods: Fifty-seven male soccer players aged 10–12 years participated in an 8-week training program (2 × week). They were randomly assigned to a balance-plyometric (BPT: n = 21), agility-plyometric (APT: n = 20) or control group (n = 16). Measures included proxies of muscle power [countermovement jump (CMJ), triple-hop-test (THT)], muscle strength [reactive strength index (RSI), maximum voluntary isometric contraction (MVIC) of handgrip, back extensors, knee extensors], agility [4-m × 9-m shuttle run, Illinois change of direction test (ICODT) with and without the ball], balance (Standing Stork, Y-Balance), and speed (10–30 m sprints). Results: Significant time × group interactions were found for CMJ, hand grip MVIC force, ICODT without a ball, agility (4 m × 9 m), standing stork balance, Y-balance, 10 and 30-m sprint. The APT pre- to post-test measures displayed large ES improvements for hand grip MVIC force, ICODT without a ball, agility test, CMJ, standing stork balance test, Y-balance test but only moderate ES improvements with the 10 and 30 m sprints. The BPT group showed small (30 m sprint), moderate (hand grip MVIC, ICODTwithout a ball) and large ES [agility (4 m × 9 m) test, CMJ, standing stork balance test, Y-balance] improvements, respectively. Conclusion: In conclusion, both training groups provided significant improvements in all measures. It is recommended that youth incorporate balance exercises into their training and progress to agility with their strength and power training.

Combination of Agility and Plyometric Training Provides Similar Training Benefits as Combined Balance and Plyometric Training in Young Soccer Players (2018)

Makhlouf, Issam ; Chaouachi, Anis ; Chaouachi, Mehdi ; Othman, Aymen Ben ; Granacher, Urs

Introduction: Studies that combined balance and resistance training induced larger performance improvements compared with single mode training. Agility exercises contain more dynamic and sport-specific movements compared with balance training. Thus, the purpose of this study was to contrast the effects of combined balance and plyometric training with combined agility and plyometric training and an active control on physical fitness in youth. Methods: Fifty-seven male soccer players aged 10–12 years participated in an 8-week training program (2 × week). They were randomly assigned to a balance-plyometric (BPT: n = 21), agility-plyometric (APT: n = 20) or control group (n = 16). Measures included proxies of muscle power [countermovement jump (CMJ), triple-hop-test (THT)], muscle strength [reactive strength index (RSI), maximum voluntary isometric contraction (MVIC) of handgrip, back extensors, knee extensors], agility [4-m × 9-m shuttle run, Illinois change of direction test (ICODT) with and without the ball], balance (Standing Stork, Y-Balance), and speed (10–30 m sprints). Results: Significant time × group interactions were found for CMJ, hand grip MVIC force, ICODT without a ball, agility (4 m × 9 m), standing stork balance, Y-balance, 10 and 30-m sprint. The APT pre- to post-test measures displayed large ES improvements for hand grip MVIC force, ICODT without a ball, agility test, CMJ, standing stork balance test, Y-balance test but only moderate ES improvements with the 10 and 30 m sprints. The BPT group showed small (30 m sprint), moderate (hand grip MVIC, ICODTwithout a ball) and large ES [agility (4 m × 9 m) test, CMJ, standing stork balance test, Y-balance] improvements, respectively. Conclusion: In conclusion, both training groups provided significant improvements in all measures. It is recommended that youth incorporate balance exercises into their training and progress to agility with their strength and power training.

Postactivation Potentiation of the Plantar Flexors Does Not Directly Translate to Jump Performance in Female Elite Young Soccer Players (2018)

Prieske, Olaf ; Maffiuletti, Nicola A. ; Granacher, Urs

Background: Infection with human immunodeficiency virus (HIV) affects muscle mass, altering independent activities of people living with HIV (PLWH). Resistance training alone (RT) or combined with aerobic exercise (AE) is linked to improved muscle mass and strength maintenance in PLWH. These exercise benefits have been the focus of different meta-analyses, although only a limited number of studies have been identified up to the year 2013/4. An up-to-date systematic review and meta-analysis concerning the effect of RT alone or combined with AE on strength parameters and hormones is of high value, since more and recent studies dealing with these types of exercise in PLWH have been published. Methods: Randomized controlled trials evaluating the effects of RT alone, AE alone or the combination of both (AERT) on PLWH was performed through five web-databases up to December 2017. Risk of bias and study quality was attained using the PEDro scale. Weighted mean difference (WMD) from baseline to post-intervention changes was calculated. The I2 statistics for heterogeneity was calculated. Results: Thirteen studies reported strength outcomes. Eight studies presented a low risk of bias. The overall change in upper body strength was 19.3 Kg (95% CI: 9.8±28.8, p< 0.001) after AERT and 17.5 Kg (95% CI: 16±19.1, p< 0.001) for RT. Lower body change was 29.4 Kg (95% CI: 18.1±40.8, p< 0.001) after RT and 10.2 Kg (95% CI: 6.7±13.8, p< 0.001) for AERT. Changes were higher after controlling for the risk of bias in upper and lower body strength and for supervised exercise in lower body strength. A significant change towards lower levels of IL-6 was found (-2.4 ng/dl (95% CI: -2.6, -2.1, p< 0.001). Conclusion: Both resistance training alone and combined with aerobic exercise showed a positive change when studies with low risk of bias and professional supervision were analyzed, improving upper and, more critically, lower body muscle strength. Also, this study found that exercise had a lowering effect on IL-6 levels in PLWH.

Postactivation Potentiation of the Plantar Flexors Does Not Directly Translate to Jump Performance in Female Elite Young Soccer Players (2018)

Prieske, Olaf ; Maffiuletti, Nicola A. ; Granacher, Urs

Background: Infection with human immunodeficiency virus (HIV) affects muscle mass, altering independent activities of people living with HIV (PLWH). Resistance training alone (RT) or combined with aerobic exercise (AE) is linked to improved muscle mass and strength maintenance in PLWH. These exercise benefits have been the focus of different meta-analyses, although only a limited number of studies have been identified up to the year 2013/4. An up-to-date systematic review and meta-analysis concerning the effect of RT alone or combined with AE on strength parameters and hormones is of high value, since more and recent studies dealing with these types of exercise in PLWH have been published. Methods: Randomized controlled trials evaluating the effects of RT alone, AE alone or the combination of both (AERT) on PLWH was performed through five web-databases up to December 2017. Risk of bias and study quality was attained using the PEDro scale. Weighted mean difference (WMD) from baseline to post-intervention changes was calculated. The I2 statistics for heterogeneity was calculated. Results: Thirteen studies reported strength outcomes. Eight studies presented a low risk of bias. The overall change in upper body strength was 19.3 Kg (95% CI: 9.8±28.8, p< 0.001) after AERT and 17.5 Kg (95% CI: 16±19.1, p< 0.001) for RT. Lower body change was 29.4 Kg (95% CI: 18.1±40.8, p< 0.001) after RT and 10.2 Kg (95% CI: 6.7±13.8, p< 0.001) for AERT. Changes were higher after controlling for the risk of bias in upper and lower body strength and for supervised exercise in lower body strength. A significant change towards lower levels of IL-6 was found (-2.4 ng/dl (95% CI: -2.6, -2.1, p< 0.001). Conclusion: Both resistance training alone and combined with aerobic exercise showed a positive change when studies with low risk of bias and professional supervision were analyzed, improving upper and, more critically, lower body muscle strength. Also, this study found that exercise had a lowering effect on IL-6 levels in PLWH.

Measuring decadal vertical land-level changes from SRTM-C (2000) and TanDEM-X (∼ 2015) in the south-central Andes (2018)

Purinton, Benjamin ; Bookhagen, Bodo

In the arctic and high mountains it is common to measure vertical changes of ice sheets and glaciers via digital elevation model (DEM) differencing. This requires the signal of change to outweigh the noise associated with the datasets. Excluding large landslides, on the ice-free earth the land-level change is smaller in vertical magnitude and thus requires more accurate DEMs for differencing and identification of change. Previously, this has required meter to submeter data at small spatial scales. Following careful corrections, we are able to measure land-level changes in gravel-bed channels and steep hillslopes in the south-central Andes using the SRTM-C (collected in 2000) and the TanDEM-X (collected from 2010 to 2015) near-global 12–30m DEMs. Long-standing errors in the SRTM-C are corrected using the TanDEM-X as a control surface and applying cosine-fit co-registration to remove ∼ 1∕10 pixel (∼ 3m) shifts, fast Fourier transform (FFT) and filtering to remove SRTM-C short- and long-wavelength stripes, and blocked shifting to remove remaining complex biases. The datasets are then differenced and outlier pixels are identified as a potential signal for the case of gravel-bed channels and hillslopes. We are able to identify signals of incision and aggradation (with magnitudes down to ∼ 3m in the best case) in two > 100km river reaches, with increased geomorphic activity downstream of knickpoints. Anthropogenic gravel excavation and piling is prominently measured, with magnitudes exceeding ±5m (up to > 10m for large piles). These values correspond to conservative average rates of 0.2 to > 0.5myr−1 for vertical changes in gravel-bed rivers. For hillslopes, since we require stricter cutoffs for noise, we are only able to identify one major landslide in the study area with a deposit volume of 16±0.15×106m3. Additional signals of change can be garnered from TanDEM-X auxiliary layers; however, these are more difficult to quantify. The methods presented can be extended to any region of the world with SRTM-C and TanDEM-X coverage where vertical land-level changes are of interest, with the caveat that remaining vertical uncertainties in primarily the SRTM-C limit detection in steep and complex topography.

Measuring decadal vertical land-level changes from SRTM-C (2000) and TanDEM-X (∼ 2015) in the south-central Andes (2018)

Purinton, Benjamin ; Bookhagen, Bodo

In the arctic and high mountains it is common to measure vertical changes of ice sheets and glaciers via digital elevation model (DEM) differencing. This requires the signal of change to outweigh the noise associated with the datasets. Excluding large landslides, on the ice-free earth the land-level change is smaller in vertical magnitude and thus requires more accurate DEMs for differencing and identification of change. Previously, this has required meter to submeter data at small spatial scales. Following careful corrections, we are able to measure land-level changes in gravel-bed channels and steep hillslopes in the south-central Andes using the SRTM-C (collected in 2000) and the TanDEM-X (collected from 2010 to 2015) near-global 12–30m DEMs. Long-standing errors in the SRTM-C are corrected using the TanDEM-X as a control surface and applying cosine-fit co-registration to remove ∼ 1∕10 pixel (∼ 3m) shifts, fast Fourier transform (FFT) and filtering to remove SRTM-C short- and long-wavelength stripes, and blocked shifting to remove remaining complex biases. The datasets are then differenced and outlier pixels are identified as a potential signal for the case of gravel-bed channels and hillslopes. We are able to identify signals of incision and aggradation (with magnitudes down to ∼ 3m in the best case) in two > 100km river reaches, with increased geomorphic activity downstream of knickpoints. Anthropogenic gravel excavation and piling is prominently measured, with magnitudes exceeding ±5m (up to > 10m for large piles). These values correspond to conservative average rates of 0.2 to > 0.5myr−1 for vertical changes in gravel-bed rivers. For hillslopes, since we require stricter cutoffs for noise, we are only able to identify one major landslide in the study area with a deposit volume of 16±0.15×106m3. Additional signals of change can be garnered from TanDEM-X auxiliary layers; however, these are more difficult to quantify. The methods presented can be extended to any region of the world with SRTM-C and TanDEM-X coverage where vertical land-level changes are of interest, with the caveat that remaining vertical uncertainties in primarily the SRTM-C limit detection in steep and complex topography.

Abrüstungsverträge in der UNO (2018)

Kane, Angela

Das dritte Working Paper in der KFG Working Paper Series analysiert Zustand und Perspektiven völkerrechtlicher Abrüstungsverträge unter der Ägide der Vereinten Nationen. Während die dreißig Jahre zwischen der Kuba-Krise und dem Fall des Eisernen Vorhangs für die Abrüstung eine erfolgreiche Periode gewesen seien, seien in den Vereinten Nationen seither außer dem Waffenhandelsvertrag keine weiteren Abrüstungsverträge abgeschlossen worden. Die gegenwärtige Stimmung sei abwartend bis negativ, obwohl es ein Nachholbedürfnis gebe, Abrüstungsverträge an die heutigen politischen Gegebenheiten sowie an den Stand der Technik anzupassen. Die Verfasserin schlägt als Lösung vor, durch eine Politik der kleinen Schritte ein besseres Abrüstungsklima zu schaffen, indem dem Diskurs auf Grundlage zusätzlicher Protokolle zu bestehenden Verträgen und notfalls auch durch ein Ausweichen auf andere Gremien eine neue Richtung verliehen werde.

Assessment of Dietary Intake of Long-Distance Race Car Drivers (2018)

Appiah-Dwomoh, Edem Korkor ; Carlsohn, Anja ; Mayer, Frank

Long-distance race car drivers are classified as athletes. The sport is physically and mentally demanding, requiring long hours of practice. Therefore, optimal dietary intake is essential for health and performance of the athlete. The aim of the study was to evaluate dietary intake and to compare the data with dietary recommendations for athletes and for the general adult population according to the German Nutrition Society (DGE). A 24-h dietary recall during a competition preparation phase was obtained from 16 male race car drivers (28.3 ± 6.1 years, body mass index (BMI) of 22.9 ± 2.3 kg/m2). The mean intake of energy, nutrients, water and alcohol was recorded. The mean energy, vitamin B2, vitamin E, folate, fiber, calcium, water and alcohol intake were 2124 ± 814 kcal/day, 1.3 ± 0.5 mg/day, 12.5 ± 9.5 mg/day, 231.0 ± 90.9 ug/day, 21.4 ± 9.4 g/day, 1104 ± 764 mg/day, 3309 ± 1522 mL/day and 0.8 ± 2.5 mL/day respectively. Our study indicated that many of the nutrients studied, including energy and carbohydrate, were below the recommended dietary intake for both athletes and the DGE.

Assessment of Dietary Intake of Long-Distance Race Car Drivers (2018)

Appiah-Dwomoh, Edem Korkor ; Carlsohn, Anja ; Frank, Mayer

Long-distance race car drivers are classified as athletes. The sport is physically and mentally demanding, requiring long hours of practice. Therefore, optimal dietary intake is essential for health and performance of the athlete. The aim of the study was to evaluate dietary intake and to compare the data with dietary recommendations for athletes and for the general adult population according to the German Nutrition Society (DGE). A 24-h dietary recall during a competition preparation phase was obtained from 16 male race car drivers (28.3 ± 6.1 years, body mass index (BMI) of 22.9 ± 2.3 kg/m2). The mean intake of energy, nutrients, water and alcohol was recorded. The mean energy, vitamin B2, vitamin E, folate, fiber, calcium, water and alcohol intake were 2124 ± 814 kcal/day, 1.3 ± 0.5 mg/day, 12.5 ± 9.5 mg/day, 231.0 ± 90.9 ug/day, 21.4 ± 9.4 g/day, 1104 ± 764 mg/day, 3309 ± 1522 mL/day and 0.8 ± 2.5 mL/day respectively. Our study indicated that many of the nutrients studied, including energy and carbohydrate, were below the recommended dietary intake for both athletes and the DGE.

Dynamic coronal mass ejection process and magnetic reconnection (2018)

Saad Hassanin, Alshaimaa

The Sun is the nearest star to the Earth. It consists of an interior and an atmosphere. The convection zone is the outermost layer of the solar interior. A flux rope may emerge as a coherent structure from the convection zone into the solar atmosphere or be formed by magnetic reconnection in the atmosphere. A flux rope is a bundle of magnetic field lines twisting around an axis field line, creating a helical shape by which dense filament material can be supported against gravity. The flux rope is also considered as the key structure of the most energetic phenomena in the solar system, such as coronal mass ejections (CMEs) and flares. These magnetic flux ropes can produce severe geomagnetic storms. In particular, to improve the ability to forecast space weather, it is important to enrich our knowledge about the dynamic formation of flux ropes and the underlying physical mechanisms that initiate their eruption, such as a CME. A confined eruption consists of a filament eruption and usually an associated are, but does not evolve into a CME; rather, the moving plasma is halted in the solar corona and usually seen to fall back. The first detailed observations of a confined filament eruption were obtained on 2002 May 27by the TRACE satellite in the 195 A band. So, in the Chapter 3, we focus on a flux rope instability model. A twisted flux rope can become unstable by entering the kink instability regime. We show that the kink instability, which occurs if the twist of a flux rope exceeds a critical value, is capable of initiating of an eruption. This model is tested against the well observed confined eruption on 2002 May 27 in a parametric magnetohydrodynamic (MHD) simulation study that comprises all phases of the event. Very good agreement with the essential observed properties is obtained, only except for a relatively poor matching of the initial filament height. Therefore, in Chapter 4, we submerge the center point of the flux rope deeper below the photosphere to obtain a flatter coronal rope section and a better matching with the initial height profile of the erupting filament. This implies a more realistic inclusion of the photospheric line tying. All basic assumptions and the other parameter settings are kept the same as in Chapter 3. This complement of the parametric study shows that the flux rope instability model can yield an even better match with the observational data. We also focus in Chapters 3 and 4 on the magnetic reconnection during the confined eruption, demonstrating that it occurs in two distinct locations and phases that correspond to the observed brightenings and changes of topology, and consider the fate of the erupting flux, which can reform a (less twisted) flux rope. The Sun also produces series of homologous eruptions, i.e. eruptions which occur repetitively in the same active region and are of similar morphology. Therefore, in Chapter 5, we employ the reformed flux rope as a new initial condition, to investigate the possibility of subsequent homologous eruptions. Free magnetic energy is built up by imposing motions in the bottom boundary, such as converging motions, leading to flux cancellation. We apply converging motions in the sunspot area, such that a small part of the flux from the sunspots with different polarities is transported toward the polarity inversion line (PIL) and cancels with each other. The reconnection associated with the cancellation process forms more helical magnetic flux around the reformed flux rope, which leads to a second and a third eruption. In this study, we obtain the first MHD simulation results of a homologous sequence of eruptions that show a transition from a confined to two ejective eruptions, based on the reformation of a flux rope after each eruption.

Experimental Study of Liquid Interfaces with Compositional Gradients (2018)

Eickelmann, Stephan Felix

The topic of this thesis is the experimental investigation of evaporating thin films on planar solid substrates and the enrichment, the crystal growth and Marangoni flows near the three phase line in the case of partially wetting mixtures of volatile and non volatile liquids. In short, it deals with the properties of planar liquid films and with those of thin liquid sections near the three phase contact line. In both cases the liquid looses continuously one component by evaporation. One topic is the rupture behavior of ultra-thin films of binary mixtures of a volatile solvent and a nonvolatile solute. It is studied how the thickness at which the film ruptures is related to the solute crystallization at the liquid/ substrate interface as soon as the solute reaches supersaturation. A universal relation between the rupture thickness and the saturation behaviour is presented. The second research subject are individual nanoparticles embedded in molecularly thin films at planar substrates. It is found that the nanoparticles cause an unexpectedly large film surface distortion (meniscus). This distortion can be measured quantitatively by conventional reflective microscopy although the nanoparticles are much smaller than the Rayleigh diffraction limit. Investigations with binary mixtures of volatile solvents and non-volatile solutes (polymers) aim at a better understanding/prediction of the final solute coverage, the timeresolved film thinning, the time-resolved solvent evaporation, and the evolution of the solute concentration within the thinning film. A quantiative theoretical description of the experimental findings is derived. Experiments of completely miscible binary mixtures of volatile liquids, which individually form continuous planar films show unexpectedly that films of mixtures are not necessarily continuous and planar. Rather, they may form surface undulations or even rupture. This is explained with surface Marangoni flows. A new method for the exceptionally fast fabrication (mm/min) of ultralong aligned diphenylalanin single crystals via dip casting is presented. It is shown how the specific evaporation conditions at the three phase line can be used for a controlled peptide crystal growth process. It is further demonstrated how the confinement inside a smalll capillary affects the peptide crystallization and how this can be understood (and used).

Boundary Value Problems on Manifolds with Singularities (2018)

Khalil, Sara

In the thesis there are constructed new quantizations for pseudo-differential boundary value problems (BVPs) on manifolds with edge. The shape of operators comes from Boutet de Monvel’s calculus which exists on smooth manifolds with boundary. The singular case, here with edge and boundary, is much more complicated. The present approach simplifies the operator-valued symbolic structures by using suitable Mellin quantizations on infinite stretched model cones of wedges with boundary. The Mellin symbols themselves are, modulo smoothing ones, with asymptotics, holomorphic in the complex Mellin covariable. One of the main results is the construction of parametrices of elliptic elements in the corresponding operator algebra, including elliptic edge conditions.

Der Integrationsprozess Kosovos in die Europäische Union (2018)

Kilgus, Tobias

Die Republik Kosovo stellt einen Beitrittskandidaten sui generis in der Erweiterungsgeschichte der Europäischen Union dar. Die besonderen Beziehungen zwischen der EU und dem "potenziellen" Kandidaten resultieren aus dem ungelösten Territorialkonflikt mit Serbien, dem unvollendeten Staatsaufbau sowie der eingeschränkten Souveränität Kosovos. Diese Einzelfallstudie untersucht Kosovos Integrationsprozess in den Staatenverbund im Kontext der Spezifika des Beitrittsaspiranten, der stockenden EU-Südosterweiterung und der multiplen EU-Krise. Davon ausgehend werden vier Zukunftsszenarien für Kosovos Integrationsverlauf, auch mit Blick auf differenzierte Integrationsoptionen, entwickelt.

Kombination von Fluoreszenzmikroskopie und Rasterkraftmikroskopie zur Aufklärung physiologischer Prozesse in lebenden Zellen (2018)

Roder, Phillip

Innerhalb dieser Doktorarbeit wurde eine neuartige Mikromanipulationstechnik für die lokale Flüssigkeitsabgabe am komplexen Drüsengewebe der Schabe P. americana charakterisiert und für die damit verbundene gezielte Manipulation von einzelnen Zellen in einem Zellkomplex (Gewebe) angewandt. Bei dieser Mikromanipulationstechnik handelt es sich um die seit 2009 bekannte nanofluidische Rasterkraftmikroskopie (FluidFM = fluidic force microscopy). Dabei werden sehr kleine mikrokanälige Rasterkraftspitzen bzw. Mikro-/Nanopipetten mit einer Öffnung zwischen 300 nm und 2 µm verwendet, mit denen es möglich ist, sehr kleine Volumina im Pikoliter- bis Femtoliter-Bereich (10-12 L – 10-15 L) gezielt und ortsgenau abzugeben. Das Ziel dieser Arbeit war die Analyse zellulärer Prozesse, wie z. B. Zell-Zell-Kommunikation oder Signalweiterleitung, zwischen benachbarten Zellen unter Zuhilfenahme der Fluoreszenzmikroskopie. Mit dieser Methode können die Zellen und ihre Bestandteile mittels vorheriger Farbstoffbeladung unter einem Mikroskop mit hohem Kontrast optisch dargestellt werden. Mit Hilfe der Fluoreszenzmikroskopie sollten schlussendlich die zellulären Reaktionen innerhalb des Gewebes nach der lokalen Manipulation visualisiert werden. Zunächst wurde die Anwendung des Systems an Luft und wässriger Umgebung beschrieben. In diesem Zusammenhang wurde eine Reinigungs- und Beladungsmethode entwickelt, mit der es möglich war, die kostspieligen Mikro-/Nanopipetten zu reinigen und anschließend mehrmals wiederzuverwenden. Hierzu wurde eine alternative Methode getestet, mit der das Diffusionsverhalten von Farbstoffmolekülen in unterschiedlichen Medien untersucht werden kann. Des Weiteren wurden die Systemparameter optimiert, welche nötig sind, um zwischen der Probenoberfläche und der Pipette einen guten Pipettenöffnungs-abschluss zu erhalten. Dieser Abschluss ist essentiell, damit die abgegebene Flüssigkeit ausschließlich in der Abgaberegion mit der Probe wechselwirkt und die darauffolgenden Reaktionen nur innerhalb des Gewebes erfolgen, da ansonsten die Zell-Zell-Signalweiterleitung zwischen den Zellen nicht eindeutig nachvollzogen werden kann. Diese interzelluläre Kommunikation wurde anhand zweier sekundärer Botenstoffe (Ca2+ und NO) untersucht. Hierbei war es möglich einzelne lokale Reaktionen zu detektieren, welche sich über weitere Zellen ausbreiteten. Schlussendlich wurde die Fertigung einer speziellen Injektionspipette beschrieben, welche an zwei biologischen Systemen getestet wurde.

Skala zur Erfassung der Beratungskompetenz im Lehramtsstudium (2018)

Kücholl, Denise ; Lazarides, Rebecca ; Westphal, Andrea ; Lohse-Bossenz, Hendrik

Die vorliegende Studie befasst sich mit der Entwicklung und Validierung einer Skala zur Erfassung der Beratungskompetenz bei Lehramtsstudierenden im Praxissemester. Befragt wurden insgesamt 200 Studierende unterschiedlicher Lehramtsstudiengänge der Universität Potsdam. Faktoranalysen zeigten, dass das Konstrukt der Beratungskompetenz in vier Subskalen differenziert werden kann (Personale Ressourcen, Kooperation und Perspektivüberahme, Berater-Skills sowie Ressourcen- und Lösungsorientierung). Bezüglich der internen Konsistenz ergaben sich für die Subskalen Werte, die insgesamt als akzeptabel bis gut einzuschätzen sind. Die Subskalen waren erwartungsgemäß positiv korreliert. Für alle vier Subskalen der Beratungskompetenz ergaben sich Zusammenhänge schwacher bis mittlerer Stärke mit den Validierungsvariablen (Selbstwirksamkeit in Beratungen, Beratungsmotivation sowie Pädagogische Vorerfahrungen). Die Ergebnisse werden bezüglich ihrer Implikationen für die Lehrkräftebildung diskutiert.

Verbindungen etablieren (2018)

Krauskopf, Karsten ; Frohn, Julia

Ausgangslage dieses Beitrags ist, dass sich Lehrkräfte für den Vorbereitungs- und Schuldienst wechselseitig aus Brandenburg und Berlin rekrutieren. Dazu widmen sich die Projekte im Rahmen der Qualitätsoffensive Lehrerbildung der Universität Potsdam und der Humboldt-Universität zu Berlin den Herausforderungen einer Lehrkräftebildung für ein inklusives Schulsystem. Dies bot Anlass, eine Vernetzung aus drei Perspektiven zu initiieren: der Projekte, der phasenübergreifenden Lehrkräftebildung und – ausblickhaft – der Verknüpfung von wissenschaftlichen und praktischen Anliegen. Auf Basis von Fokusgruppengesprächen mit Expert_innen der zweiten Ausbildungsphase aus Berlin und Brandenburg sucht dieser Beitrag explorativ Erfahrungen und Erwartungen aus Perspektive der zweiten Phase zu kategorisieren. Die gewonnenen Kategorien werden knapp dargestellt und diskutiert. Dies soll auch den Dialog zwischen QLB-Projekten sowie erster und zweiter Phase der Lehrkräftebildung erweitern.

Zusammenarbeit von Lehrkräften, Lehramtsstudierenden und Wissenschaftler_innen (2018)

Jennek, Julia ; Kleemann, Katrin ; Vock, Miriam

Die Zusammenarbeit von Universität, Lehramtsstudierenden und Schulen gelingt, wenn die besonderen Bedürfnisse der drei Partner beachtet werden. So wünschen sich Schulen Entlastung und Unterstützung bei ihrer Weiterentwicklung, Studierende wollen ihr zukünftiges Tätigkeitsfeld näher kennenlernen und sich ausprobieren, während Wissenschaftler_innen eigene Forschungsinteressen vertreten. Um eine gute Netzwerkarbeit sicherzustellen, sind insbesondere gegenseitiges Vertrauen, gute Kommunikation und gemeinsame Ziele von besonderer Bedeutung. Im Beitrag wird der Ansatz des Projekts „Campusschulen“ vorgestellt, welches die Vernetzung von Lehrkräften, Lehramtsstudierenden und Wissenschaftler_ innen zu allerseitigem Vorteil ermöglichen soll. Es wird die konkrete Netzwerkarbeit an einem Beispiel vorgestellt sowie die übergeordneten Aufgaben der Projektkoordination, die das Gelingen der Vernetzung unterstützt, erläutert und diskutiert.

Internationalisierung der Lehrerbildung an der Universität Potsdam (2018)

Ahlgrimm, Frederik ; Heck, Sebastian

In den vergangenen Jahren wurde immer wieder gefordert, dass die Lehrerbildung an deutschen Hochschulen stärker internationalisiert werden solle. Die Universität Potsdam hat sich als Teil ihrer Internationalisierungsstrategie zur Aufgabe gemacht, die internationale Dimension ihres renommierten Modells der Lehrerbildung zu fördern. In diesem Beitrag wird dargestellt, wie im Rahmen des Teilprojektes Internationalisierung der QLB – ausgehend vom politisch-gesellschaftlichen Kontext und dem Status Quo im Bereich der Lehrerbildung – zunächst ein internes Verständnis von Internationalisierung entwickelt und darauf aufbauend eine Strategie mit konkreten Maßnahmen abgeleitet wurde. Der Implementierungsprozess führte zu Vernetzungen sowohl innerhalb der Universität als auch mit externen und internationalen Akteuren im Bildungswesen. Die vorgestellten Maßnahmen können auch für andere Hochschulen von Interesse sein.

Lehrerbildung und Schulpraktische Studien im Spiegel eines Spiralcurriculums (2018)

Tosch, Frank

Den fünf bildungswissenschaftlichen bzw. fachdidaktischen Praktika (Schulpraktische Studien) der Potsdamer Lehrerbildung liegt das Modell eines Spiralcurriculums zugrunde. Der professionelle Kompetenzerwerb wird als längerfristiger, nicht abschließbarer Prozess in beiden Phasen der Lehrerbildung sowie im tertiären Bereich begriffen. Im Beitrag werden einige historisch-systematische Positionen des Spiralcurriculums verortet und auf die Herausforderungen der Lehrerbildung gespiegelt. Die Gesamtheit der curricularen Ziele, Inhalte und Methoden aller Schulpraktischen Studien wird im Kontext des Spiralcurriculums entlang von drei tragenden Leitlinien: der fächerspezifischen, der fächerübergreifenden Erkenntnisgewinnung sowie der individuellen Kompetenzentwicklung systematisiert und vernetzt. Als Entwicklungsaufgabe wird ein „Spiralcurriculum Lehrerbildung“ benannt, das auf der Angebotsseite ein abgestimmtes Curriculum aller beteiligten Disziplinen etabliert und zugleich den individuellen Kompetenzerwerb der Studierenden als Zielmaxime systematisch im Blick hat.

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