Organic matter quality structures benthic fatty acid patterns and the abundance of fungi and bacteria in temperate lakes
- Benthic microbial communities (BMCs) play important roles in the carbon cycle of lakes, and benthic littoral zones in particular have been previously highlighted as biogeochemical hotspots. Dissolved organic matter (DOM) presents the major carbon pool in lakes, and although the effect of DOM composition on the pelagic microbial community composition is widely accepted, little is known about its effect on BMCs, particularly aquatic fungi. Therefore, we investigated the composition of benthic littoral microbial communities in twenty highly diverse lakes in northeast Germany. DOM quality was analyzed via size exclusion chromatography (SEC), fluorescence parallel factor analyses (PRAFACs) and UV-Vis spectroscopy. We determined the BMC composition and biomass using phospholipid-derived fatty acids (PLFA) and extended the interpretation to the analysis of fungi by applying a Bayesian mixed model. We present evidence that the quality of DOM structures the BMCs, which are dominated by heterotrophic bacteria and show low fungal biomass. TheBenthic microbial communities (BMCs) play important roles in the carbon cycle of lakes, and benthic littoral zones in particular have been previously highlighted as biogeochemical hotspots. Dissolved organic matter (DOM) presents the major carbon pool in lakes, and although the effect of DOM composition on the pelagic microbial community composition is widely accepted, little is known about its effect on BMCs, particularly aquatic fungi. Therefore, we investigated the composition of benthic littoral microbial communities in twenty highly diverse lakes in northeast Germany. DOM quality was analyzed via size exclusion chromatography (SEC), fluorescence parallel factor analyses (PRAFACs) and UV-Vis spectroscopy. We determined the BMC composition and biomass using phospholipid-derived fatty acids (PLFA) and extended the interpretation to the analysis of fungi by applying a Bayesian mixed model. We present evidence that the quality of DOM structures the BMCs, which are dominated by heterotrophic bacteria and show low fungal biomass. The fungal biomass increases when the DOM pool is processed by microorganisms of allochthonous origin, whereas the opposite is true for bacteria.…
Author details: | Robert Taube, Lars GanzertORCiDGND, Hans-Peter GrossartORCiDGND, Gerd GleixnerORCiD, Katrin PremkeORCiD |
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DOI: | https://doi.org/10.1016/j.scitotenv.2017.07.256 |
ISSN: | 0048-9697 |
ISSN: | 1879-1026 |
Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/28818662 |
Title of parent work (English): | The science of the total environment : an international journal for scientific research into the environment and its relationship with man |
Publisher: | Elsevier |
Place of publishing: | Amsterdam |
Publication type: | Article |
Language: | English |
Date of first publication: | 2017/09/14 |
Publication year: | 2017 |
Release date: | 2022/04/07 |
Tag: | Aquatic fungi; FASTAR; PARAFAC; PLFA; Size exclusion chromatography (SEC); Stable isotopes |
Volume: | 610 |
Number of pages: | 13 |
First page: | 469 |
Last Page: | 481 |
Funding institution: | Mycolink project [SAW-2014-IGB]; Pact for Innovation and Research of the Gottfried Wilhelm Leibniz Scientific Community; German Research Foundation project MicroPrime (DFG) [GR1540/23-1] |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
DDC classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Peer review: | Referiert |
Publishing method: | Open Access / Green Open-Access |