Anika Küken, Frederik Sommer, Liliya Yaneva-Roder, Luke C. M. Mackinder, Melanie Hoehne, Stefan Geimer, Martin C. Jonikas, Michael Schroda, Mark Stitt, Zoran Nikoloski, Tabea Mettler-Altmann
- Cells and organelles are not homogeneous but include microcompartments that alter the spatiotemporal characteristics of cellular processes. The effects of microcompartmentation on metabolic pathways are however difficult to study experimentally. The pyrenoid is a microcompartment that is essential for a carbon concentrating mechanism (CCM) that improves the photosynthetic performance of eukaryotic algae. Using Chlamydomonas reinhardtii, we obtained experimental data on photosynthesis, metabolites, and proteins in CCM-induced and CCM-suppressed cells. We then employed a computational strategy to estimate how fluxes through the Calvin-Benson cycle are compartmented between the pyrenoid and the stroma. Our model predicts that ribulose-1,5-bisphosphate (RuBP), the substrate of Rubisco, and 3-phosphoglycerate (3PGA), its product, diffuse in and out of the pyrenoid, respectively, with higher fluxes in CCM-induced cells. It also indicates that there is no major diffusional barrier to metabolic flux between the pyrenoid and stroma. OurCells and organelles are not homogeneous but include microcompartments that alter the spatiotemporal characteristics of cellular processes. The effects of microcompartmentation on metabolic pathways are however difficult to study experimentally. The pyrenoid is a microcompartment that is essential for a carbon concentrating mechanism (CCM) that improves the photosynthetic performance of eukaryotic algae. Using Chlamydomonas reinhardtii, we obtained experimental data on photosynthesis, metabolites, and proteins in CCM-induced and CCM-suppressed cells. We then employed a computational strategy to estimate how fluxes through the Calvin-Benson cycle are compartmented between the pyrenoid and the stroma. Our model predicts that ribulose-1,5-bisphosphate (RuBP), the substrate of Rubisco, and 3-phosphoglycerate (3PGA), its product, diffuse in and out of the pyrenoid, respectively, with higher fluxes in CCM-induced cells. It also indicates that there is no major diffusional barrier to metabolic flux between the pyrenoid and stroma. Our computational approach represents a stepping stone to understanding microcompartmentalized CCM in other organisms.…
MetadatenAuthor details: | Anika KükenORCiDGND, Frederik Sommer, Liliya Yaneva-Roder, Luke C. M. MackinderORCiD, Melanie Hoehne, Stefan Geimer, Martin C. Jonikas, Michael SchrodaORCiD, Mark StittORCiDGND, Zoran NikoloskiORCiDGND, Tabea Mettler-AltmannORCiD |
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DOI: | https://doi.org/10.7554/eLife.37960 |
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ISSN: | 2050-084X |
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Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/30306890 |
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Title of parent work (English): | eLife |
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Publisher: | eLife Sciences Publications |
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Place of publishing: | Cambridge |
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Publication type: | Article |
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Language: | English |
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Year of first publication: | 2018 |
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Publication year: | 2018 |
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Release date: | 2021/09/13 |
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Volume: | 7 |
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Number of pages: | 23 |
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Funding institution: | Deutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [EXC 1028]; Bundesministerium fur Bildung und ForschungFederal Ministry of Education & Research (BMBF) [FKZ0313924]; Max-Planck-GesellschaftMax Planck Society; National Science FoundationNational Science Foundation (NSF) [EF-1105617]; National Institutes of HealthUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [DP2-GM-119137]; Simons Foundation [55108535] |
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Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
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Peer review: | Referiert |
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Publishing method: | Open Access / Gold Open-Access |
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| DOAJ gelistet |
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External remark: | Zweitveröffentlichung in der Schriftenreihe Postprints der Universität Potsdam : Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe ; 1122 |
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