The Role of the Nucleotides in the Insertion of the bis-Molybdopterin Guanine Dinucleotide Cofactor into apo-Molybdoenzymes
- The role of the GMP nucleotides of the bis-molybdopterin guanine dinucleotide (bis-MGD) cofactor of the DMSO reductase family has long been a subject of discussion. The recent characterization of the bis-molybdopterin (bis-Mo-MPT) cofactor present in the E. coli YdhV protein, which differs from bis-MGD solely by the absence of the nucleotides, now enables studying the role of the nucleotides of bis-MGD and bis-MPT cofactors in Moco insertion and the activity of molybdoenzymes in direct comparison. Using the well-known E. coli TMAO reductase TorA as a model enzyme for cofactor insertion, we were able to show that the GMP nucleotides of bis-MGD are crucial for the insertion of the bis-MGD cofactor into apo-TorA.
| Author details: | Kim Tiedemann, Chantal Iobbi-NivolORCiD, Silke LeimkühlerORCiDGND |
|---|---|
| DOI: | https://doi.org/10.3390/molecules27092993 |
| ISSN: | 1420-3049 |
| Title of parent work (English): | Molecules |
| Publisher: | MDPI |
| Place of publishing: | Basel, Schweiz |
| Further contributing person(s): | Roberto Fernandez-Lafuente |
| Publication type: | Article |
| Language: | English |
| Date of first publication: | 2022/05/06 |
| Publication year: | 2022 |
| Release date: | 2022/09/27 |
| Tag: | TMAO reductase; bis-MGD; chaperone; molybdenum cofactor |
| Volume: | 27 |
| Article number: | 2993 |
| Print run: | 9 |
| Number of pages: | 15 |
| First page: | 1 |
| Last Page: | 15 |
| Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
| Extern / Extern | |
| DDC classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
| Peer review: | Referiert |
| Grantor: | Publikationsfonds der Universität Potsdam |
| Publishing method: | Open Access / Gold Open-Access |
| License (German): |  CC-BY - Namensnennung 4.0 International |
| External remark: | Zweitveröffentlichung in der Schriftenreihe Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe ; 1268 |
