- search hit 1 of 1
Surface immobilization strategies for tyrosinase as biocatalyst applicable to polymer network synthesis
- Enzymes have recently attracted increasing attention in material research based on their capacity to catalyze the conversion of polymer-bound moieties for synthesizing polymer networks, particularly bulk hydrogels. hi this study. the surface immobilization of a relevant enzyme. mushroom tyrosinase, should be explored using glass as model surface. In a first step. the glass support was functionalized with silanes to introduce either amine or carboxyl groups, as confirmed e.g. by X-ray photoelectron spectroscopy. By applying glutaraldehyde and EDC/NHS chemistry, respectively, surfaces have been activated for subsequent successful coupling of tyrosinase. Via protein hydrolysis and amino acid characterization by HPLC, the quantity of bound tyrosinase was shown to correspond to a full surface coverage. Based on the visualized enzymatic conversion of a test substrate at the glass support. the functionalized surfaces may be explored for surface-associated material synthesis in the future.
Author details: | Christian WischkeORCiDGND, Elen Baehr, Miroslava Racheva, Matthias HeuchelORCiDGND, Thomas Weigel, Andreas LendleinORCiDGND |
---|---|
DOI: | https://doi.org/10.1557/adv.2018.630 |
ISSN: | 2059-8521 |
Title of parent work (English): | MRS Advances |
Publisher: | Cambridge Univ. Press |
Place of publishing: | New York |
Publication type: | Article |
Language: | English |
Date of first publication: | 2018/12/11 |
Publication year: | 2018 |
Release date: | 2022/03/02 |
Volume: | 3 |
Issue: | 63 |
Number of pages: | 7 |
First page: | 3875 |
Last Page: | 3881 |
Funding institution: | German Federal Ministry of Education and Research via the MIE project [031A095B]; Helmholtz AssociationHelmholtz Association |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
DDC classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Peer review: | Referiert |