The Tinkerbell (Tink) Mutation Identifies the Dual-Specificity MAPK Phosphatase INDOLE-3-BUTYRIC ACID-RESPONSE5 (IBR5) as a Novel Regulator of Organ Size in Arabidopsis
- Mitogen-activated dual-specificity MAPK phosphatases are important negative regulators in the MAPK signalling pathways responsible for many essential processes in plants. In a screen for mutants with reduced organ size we have identified a mutation in the active site of the dual-specificity MAPK phosphatase INDOLE-3-BUTYRIC ACID-RESPONSE5 (IBR5) that we named tinkerbell (tink) due to its small size. Analysis of the tink mutant indicates that IBR5 acts as a novel regulator of organ size that changes the rate of growth in petals and leaves. Organ size and shape regulation by IBR5 acts independently of the KLU growth-regulatory pathway. Microarray analysis of tink/ibr5-6 mutants identified a likely role for this phosphatase in male gametophyte development. We show that IBR5 may influence the size and shape of petals through auxin and TCP growth regulatory pathways.
Author details: | Kim L. JohnsonORCiDGND, Sascha Ramm, Christian KappelORCiDGND, Sally Ward, Ottoline Leyser, Tomoaki Sakamoto, Tetsuya Kurata, Michael W. Bevan, Michael LenhardORCiDGND |
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DOI: | https://doi.org/10.1371/journal.pone.0131103 |
ISSN: | 1932-6203 |
Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/26147117 |
Title of parent work (English): | PLoS one |
Publisher: | PLoS |
Place of publishing: | San Fransisco |
Publication type: | Article |
Language: | English |
Year of first publication: | 2015 |
Publication year: | 2015 |
Release date: | 2017/03/27 |
Volume: | 10 |
Issue: | 7 |
Number of pages: | 17 |
Funding institution: | Biotechnology and Biological Sciences Research Council; Australian Research Council, Centre of Excellence in Plant Cell Walls |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
Peer review: | Referiert |
Publishing method: | Open Access |
External remark: | Zweitveröffentlichung in der Schriftenreihe Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe ; 427 |