VMP1-deficient Chlamydomonas exhibits severely aberrant cell morphology and disrupted cytokinesies
- Background: The versatile Vacuole Membrane Protein 1 (VMP1) has been previously investigated in six species. It has been shown to be essential in macroautophagy, where it takes part in autophagy initiation. In addition, VMP1 has been implicated in organellar biogenesis; endo-, exo- and phagocytosis, and protein secretion; apoptosis; and cell adhesion. These roles underly its proven involvement in pancreatitis, diabetes and cancer in humans. Results: In this study we analyzed a VMP1 homologue from the green alga Chlamydomonas reinhardtii. CrVMP1 knockdown lines showed severe phenotypes, mainly affecting cell division as well as the morphology of cells and organelles. We also provide several pieces of evidence for its involvement in macroautophagy.
| Author details: | Hezi Tenenboim, Julia Smirnova, Lothar WillmitzerORCiDGND, Martin SteupORCiDGND, Yariv Brotman |
|---|---|
| DOI: | https://doi.org/10.1186/1471-2229-14-121 |
| ISSN: | 1471-2229 |
| Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/24885763 |
| Title of parent work (English): | BMC plant biology |
| Publisher: | BioMed Central |
| Place of publishing: | London |
| Publication type: | Article |
| Language: | English |
| Year of first publication: | 2014 |
| Publication year: | 2014 |
| Release date: | 2017/03/27 |
| Tag: | Autophagy; Cytokinesis; VMP1 |
| Volume: | 14 |
| Number of pages: | 13 |
| Funding institution: | Investitionsbank des Landes Brandenburg (ILB) [80139585] |
| Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
| Peer review: | Referiert |
| Publishing method: | Open Access |
