The biosynthesis of the molybdenum cofactors in Escherichia coli
- The biosynthesis of the molybdenum cofactor (Moco) is highly conserved among all kingdoms of life. In all molybdoenzymes containing Moco, the molybdenum atom is coordinated to a dithiolene group present in the pterin-based 6-alkyl side chain of molybdopterin (MPT). In general, the biosynthesis of Moco can be divided into four steps in in bacteria: (i) the starting point is the formation of the cyclic pyranopterin monophosphate (cPMP) from 5 '-GTP, (ii) in the second step the two sulfur atoms are inserted into cPMP leading to the formation of MPT, (iii) in the third step the molybdenum atom is inserted into MPT to form Moco and (iv) in the fourth step bis-Mo-MPT is formed and an additional modification of Moco is possible with the attachment of a nucleotide (CMP or GMP) to the phosphate group of MPT, forming the dinucleotide variants of Moco. This review presents an update on the well-characterized Moco biosynthesis in the model organism Escherichia coli including novel discoveries from the recent years.
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| Author details: | Silke LeimkühlerORCiDGND |
|---|---|
| URN: | urn:nbn:de:kobv:517-opus4-516559 |
| DOI: | https://doi.org/10.25932/publishup-51655 |
| ISSN: | 1866-8372 |
| Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/32239579 |
| Title of parent work (German): | Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe |
| Publication series (Volume number): | Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe (1433) |
| Publication type: | Postprint |
| Language: | English |
| Date of first publication: | 2020/04/01 |
| Publication year: | 2020 |
| Publishing institution: | Universität Potsdam |
| Release date: | 2024/03/11 |
| Tag: | biotin sulfoxide reductase; converting factor; crystal-structure; formate dehydrogenase; in-vitro-synthesis; molybdopterin synthase; n-oxide reductase; periplasmic nitrate reductase; rhodobacter-capsulatus; xanthine dehydrogenase |
| Issue: | 6 |
| Number of pages: | 22 |
| Source: | Environ Microbiol. (2020) 22(6), 2007–2026. https://doi.org/10.1111/1462-2920.15003 |
| Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
| DDC classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
| Peer review: | Referiert |
| Publishing method: | Open Access / Green Open-Access |
| License (German): |  CC-BY - Namensnennung 4.0 International |
| External remark: | Bibliographieeintrag der Originalveröffentlichung/Quelle |
