Electrode-based AC electrokinetics of proteins
- Employing electric phenomena for the spatial manipulation of bioparticles from whole cells down to dissolved molecules has become a useful tool in biotechnology and analytics. AC electrokinetic effects like dielectrophoresis and AC electroosmosis are increasingly used to concentrate, separate and immobilize DNA and proteins. With the advance of photolithographical micro- and nanofabrication methods, novel or improved bioanalytical applications benefit from concentrating analytes, signal enhancement and locally controlled immobilization by AC electrokinetic effects. In this review of AC electrokinetics of proteins, the respective studies are classified according to their different electrode geometries: individual electrode pairs, interdigitated electrodes, quadrupole electrodes, and 3D configurations of electrode arrays. Known advantages and disadvantages of each layout are discussed.
Author details: | Eva-Maria LauxGND, Frank Fabian BierORCiDGND, Ralph HölzelORCiDGND |
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DOI: | https://doi.org/10.1016/j.bioelechem.2017.11.010 |
ISSN: | 1567-5394 |
ISSN: | 1878-562X |
Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/29182911 |
Title of parent work (English): | Bioelectrochemistry : official journal of the Bioelectrochemical Society ; an international journal devoted to electrochemical aspects of biology and biological aspects of electrochemistry |
Subtitle (English): | a mini-review |
Publisher: | Elsevier B.V. |
Place of publishing: | Amsterdam |
Publication type: | Review |
Language: | English |
Date of first publication: | 2017/11/23 |
Publication year: | 2018 |
Release date: | 2022/01/03 |
Tag: | AC electrokinetics; Dielectrophoresis; Electrodes; Electroosmosis; Proteins |
Volume: | 120 |
Number of pages: | 7 |
First page: | 76 |
Last Page: | 82 |
Funding institution: | European Regional Development Fund (ERDF)European Union (EU); Brandenburg Ministry of Sciences, Research and Cultural Affairs (MWFK) [85004117]; German Research Foundation (DFG)German Research Foundation (DFG) [SPP 1857 HO1298/4-1] |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
DDC classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Peer review: | Referiert |