Dielectric Spectroscopy of Biomolecules up to 110 GHz
- Radio-frequency fields in the GHz range are increasingly applied in biotechnology and medicine. In order to fully exploit both their potential and their risks detailed information about the dielectric properties of biological material is needed. For this purpose a measuring system is presented that allows the acquisition of complex dielectric spectra over 4 frequency decade up to 110 GHz. Routines for calibration and for data evaluation according to physicochemical interaction models have been developed. The frequency dependent permittivity and dielectric loss of some proteins and nucleic acids, the main classes of biomolecules, and of their sub-units have been determined. Dielectric spectra are presented for the amino acid alanine, the proteins lysozyme and haemoglobin, the nucleotides AMP and ATP, and for the plasmid pET-21, which has been produced by bacterial culture. Characterisation of a variety of biomolecules is envisaged, as is the application to studies on protein structure and function.
Author details: | Eva-Maria LauxGND, Elena ErmilovaGND, Daniel Pannwitz, Jessica Gibbons, Ralph HölzelORCiDGND, Frank Fabian BierORCiDGND |
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DOI: | https://doi.org/10.1515/freq-2018-0010 |
ISSN: | 0016-1136 |
ISSN: | 2191-6349 |
Title of parent work (English): | Frequenz |
Publisher: | De Gruyter |
Place of publishing: | Berlin |
Publication type: | Article |
Language: | English |
Date of first publication: | 2018/03/26 |
Publication year: | 2018 |
Release date: | 2022/01/14 |
Tag: | DNA; amino acid; dielectric; permittivity; plasmid; protein; spectroscopy |
Volume: | 72 |
Issue: | 3-4 |
Number of pages: | 6 |
First page: | 135 |
Last Page: | 140 |
Funding institution: | Ministerium fur Wissenschaft, Forschung und Kultur [10.13039/501100004581, StaF]; Deutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [10.13039/501100001659, SPP1857, HO1298/4-1] |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
DDC classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Peer review: | Referiert |