Selection of protein-protein interactions of desired affinities with a bandpass circuit
- We have developed a genetic circuit in Escherichia coli that can be used to select for protein-protein interactions of different strengths by changing antibiotic concentrations in the media. The genetic circuit links protein-protein interaction strength to beta-lactamase activity while simultaneously imposing tuneable positive and negative selection pressure for beta-lactamase activity. Cells only survive if they express interacting proteins with affinities that fall within set high- and low-pass thresholds; i.e. the circuit therefore acts as a bandpass filter for protein-protein interactions. We show that the circuit can be used to recover protein-protein interactions of desired affinity from a mixed population with a range of affinities. The circuit can also be used to select for inhibitors of protein-protein interactions of defined strength. (C) 2018 Elsevier Ltd. All rights reserved.
Verfasserangaben: | Katherine Emily BrechunORCiD, Katja Maren ArndtORCiDGND, G. Andrew WoolleyORCiD |
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DOI: | https://doi.org/10.1016/j.jmb.2018.11.011 |
ISSN: | 0022-2836 |
ISSN: | 1089-8638 |
Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/30448232 |
Titel des übergeordneten Werks (Englisch): | Journal of molecular biology : JMB |
Verlag: | Elsevier |
Verlagsort: | London |
Publikationstyp: | Wissenschaftlicher Artikel |
Sprache: | Englisch |
Datum der Erstveröffentlichung: | 15.11.2018 |
Erscheinungsjahr: | 2018 |
Datum der Freischaltung: | 20.04.2021 |
Freies Schlagwort / Tag: | biological engineering; genetic circuit; protein-protein interactions; selection system; synthetic biology; twin-arginine translocation |
Band: | 431 |
Ausgabe: | 2 |
Seitenanzahl: | 10 |
Erste Seite: | 391 |
Letzte Seite: | 400 |
Fördernde Institution: | National Institutes of HealthUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [R33MH105724]; Natural Sciences and Engineering Research Council of CanadaNatural Sciences and Engineering Research Council of Canada; German Academic Exchange Service (DAAD) Deutscher Akademischer AustauschdienstDeutscher Akademischer Austausch Dienst (DAAD) |
Organisationseinheiten: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
DDC-Klassifikation: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Peer Review: | Referiert |
Publikationsweg: | Open Access / Green Open-Access |