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A hydrogen-driven biocatalytic approach to recycling synthetic analogues of NAD(P)H

  • We demonstrate a recycling system for synthetic nicotinamide cofactor analogues using a soluble hydrogenase with turnover number of >1000 for reduction of the cofactor analogues by H-2. Coupling this system to an ene reductase, we show quantitative conversion of N-ethylmaleimide to N-ethylsuccinimide. The biocatalyst system retained >50% activity after 7 h.

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Author details:Holly A. ReeveORCiD, Jake NicholsonORCiD, Farieha Altaf, Thomas H. LonsdaleORCiD, Janina PreisslerGND, Lars LauterbachORCiDGND, Oliver LenzORCiDGND, Silke LeimkühlerORCiDGND, Frank HollmannORCiDGND, Caroline E. PaulORCiDGND, Kylie A. VincentORCiD
DOI:https://doi.org/10.1039/d2cc02411j
ISSN:1359-7345
ISSN:1364-548X
Pubmed ID:https://pubmed.ncbi.nlm.nih.gov/36047350
Title of parent work (English):Chemical communications : ChemComm
Publisher:Royal Society of Chemistry
Place of publishing:Cambridge
Publication type:Article
Language:English
Date of first publication:2022/08/18
Publication year:2022
Release date:2024/05/31
Volume:58
Issue:75
Number of pages:4
First page:10540
Last Page:10543
Funding institution:Engineering and Physical Sciences Research Council (EPSRC) IB Catalyst; Award [EP/N013514/1]; NWO VENI grant [722.015.011]; Deutsche; Forschungsgemeinschaft (DFG, German Research Foundation) under Germany's; Excellence Strategy [EXC 2008-390540038]
Organizational units:Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie
Peer review:Referiert
Publishing method:Open Access / Hybrid Open-Access
License (German):License LogoCC-BY - Namensnennung 4.0 International
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