Detecting carbohydrate–lectin interactions using a fluorescent probe based on DBD dyes
- Herein we present an efficient synthesis of a biomimetic probe with modular construction that can be specifically bound by the mannose binding FimH protein – a surface adhesion protein of E. coli bacteria. The synthesis combines the new and interesting DBD dye with the carbohydrate ligand mannose via a Click reaction. We demonstrate the binding to E. coli bacteria over a large concentration range and also present some special characteristics of those molecules that are of particular interest for the application as a biosensor. In particular, the mix-and-measure ability and the very good photo-stability should be highlighted here.
Author details: | Pablo WessigORCiDGND, Denise Bader, Dennis Tobias KlierGND, Cornelia Hettrich, Frank Fabian BierORCiDGND |
---|---|
URN: | urn:nbn:de:kobv:517-opus4-394382 |
Publication series (Volume number): | Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe (314) |
Publication type: | Postprint |
Language: | English |
Date of first publication: | 2016/01/14 |
Publication year: | 2016 |
Publishing institution: | Universität Potsdam |
Release date: | 2017/03/30 |
Tag: | assay; binding; conformational-changes; green-i; protein |
Number of pages: | 4 |
First page: | 1235 |
Last Page: | 1238 |
Source: | Analytical methods (2016) Nr. 8, S. 1235-1238. - DOI: 10.1039/C5AY02991K |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
Peer review: | Referiert |
Publishing method: | Open Access |
License (English): | Creative Commons - Namensnennung 3.0 Unported |