Live cell flattening
- Eukaryotic cell flattening is valuable for improving microscopic observations, ranging from bright field (BF) to total internal reflection fluorescence (TIRF) microscopy. Fundamental processes, such as mitosis and in vivo actin polymerization, have been investigated using these techniques. Here, we review the well known agar overlayer protocol and the oil overlay method. In addition, we present more elaborate microfluidics-based techniques that provide us with a greater level of control. We demonstrate these techniques on the social amoebae Dictyostelium discoideum, comparing the advantages and disadvantages of each method.
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| Author details: | Christian Westendorf, Albert J. Bae, Christoph Erlenkamper, Edouard Galland, Carl Franck, Eberhard Bodenschatz, Carsten BetaORCiDGND |
|---|---|
| URN: | urn:nbn:de:kobv:517-opus4-428311 |
| DOI: | https://doi.org/10.25932/publishup-42831 |
| ISSN: | 1866-8372 |
| Title of parent work (English): | Postprints der Universität Potsdam : Mathematisch Naturwissenschaftliche Reihe |
| Subtitle (English): | traditional and novel approaches |
| Publication series (Volume number): | Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe (835) |
| Publication type: | Postprint |
| Language: | English |
| Date of first publication: | 2020/03/05 |
| Publication year: | 2010 |
| Publishing institution: | Universität Potsdam |
| Release date: | 2020/03/05 |
| Tag: | PDMS; lower channel; microfluidic device; total internal reflection fluorescence; total internal reflection fluorescence microscopy |
| Issue: | 835 |
| Number of pages: | 17 |
| Source: | PMC Biophysics 3 (2010) 9 DOI: 10.1186/1757-5036-3-9 |
| Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät |
| DDC classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
| Peer review: | Referiert |
| Publishing method: | Open Access |
| License (English): |  Creative Commons - Namensnennung 2.0 Generic |
