Simple and robust
- A spectrum of 7562 publications on Molecularly Imprinted Polymers (MIPs) has been presented in literature within the last ten years (Scopus, September 7, 2020). Around 10 % of the papers published on MIPs describe the recognition of proteins. The straightforward synthesis of MIPs is a significant advantage as compared with the preparation of enzymes or antibodies. MIPs have been synthesized from only one up to six functional monomers while proteins are made up of 20 natural amino acids. Furthermore, they can be synthesized against structures of low immunogenicity and allow multi-analyte measurements via multi-target synthesis. Electrochemical methods allow simple polymer synthesis, removal of the template and readout. Among the different sensor configurations electrochemical MIP-sensors provide the broadest spectrum of protein analytes. The sensitivity of MIP-sensors is sufficiently high for biomarkers in the sub-nanomolar region, nevertheless the cross-reactivity of highly abundant proteins in human serum is still a challenge. MIPsA spectrum of 7562 publications on Molecularly Imprinted Polymers (MIPs) has been presented in literature within the last ten years (Scopus, September 7, 2020). Around 10 % of the papers published on MIPs describe the recognition of proteins. The straightforward synthesis of MIPs is a significant advantage as compared with the preparation of enzymes or antibodies. MIPs have been synthesized from only one up to six functional monomers while proteins are made up of 20 natural amino acids. Furthermore, they can be synthesized against structures of low immunogenicity and allow multi-analyte measurements via multi-target synthesis. Electrochemical methods allow simple polymer synthesis, removal of the template and readout. Among the different sensor configurations electrochemical MIP-sensors provide the broadest spectrum of protein analytes. The sensitivity of MIP-sensors is sufficiently high for biomarkers in the sub-nanomolar region, nevertheless the cross-reactivity of highly abundant proteins in human serum is still a challenge. MIPs for proteins offer innovative tools not only for clinical and environmental analysis, but also for bioimaging, therapy and protein engineering.…
Author details: | Aysu YarmanORCiDGND, Sevinç KurbanoğluORCiD, Ingo ZebgerORCiDGND, Frieder W. SchellerORCiDGND |
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DOI: | https://doi.org/10.1016/j.snb.2020.129369 |
ISSN: | 0925-4005 |
ISSN: | 1873-3077 |
Title of parent work (English): | Sensors and actuators : B, Chemical : an international journal devoted to research and development of chemical transducers |
Subtitle (English): | the claims of protein sensing by molecularly imprinted polymers |
Publisher: | Elsevier Science |
Place of publishing: | Amsterdam [u.a.] |
Publication type: | Article |
Language: | English |
Date of first publication: | 2021/03/01 |
Publication year: | 2021 |
Release date: | 2024/04/10 |
Tag: | Biomimetic sensors; Functional scaffolds; Molecularly imprinted polymer; Plastibodies; Proteins |
Volume: | 330 |
Article number: | 129369 |
Number of pages: | 12 |
Funding institution: | Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany's Excellence StrategyGerman Research Foundation (DFG) [EXC 2008/1, 390540038] |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
DDC classification: | 5 Naturwissenschaften und Mathematik / 53 Physik / 530 Physik |
6 Technik, Medizin, angewandte Wissenschaften / 62 Ingenieurwissenschaften / 620 Ingenieurwissenschaften und zugeordnete Tätigkeiten | |
Peer review: | Referiert |