Yanjun Yu, Shenjie Wu, Jacqueline Nowak, Guangda Wang, Libo Han, Zhidi Feng, Amelie Mendrinna, Yinping Ma, Huan Wang, Xiaxia Zhang, Juan Tian, Li Dong, Zoran Nikoloski, Staffan Persson, Zhaosheng Kong
- Cotton (Gossypium hirsutum) fibres consist of single cells that grow in a highly polarized manner, assumed to be controlled by the cytoskeleton(1-3). However, how the cytoskeletal organization and dynamics underpin fibre development remains unexplored. Moreover, it is unclear whether cotton fibres expand via tip growth or diffuse growth(2-4). We generated stable transgenic cotton plants expressing fluorescent markers of the actin and microtubule cytoskeleton. Live-cell imaging revealed that elongating cotton fibres assemble a cortical filamentous actin network that extends along the cell axis to finally form actin strands with closed loops in the tapered fibre tip. Analyses of F-actin network properties indicate that cotton fibres have a unique actin organization that blends features of both diffuse and tip growth modes. Interestingly, typical actin organization and endosomal vesicle aggregation found in tip-growing cell apices were not observed in fibre tips. Instead, endomembrane compartments were evenly distributed along theCotton (Gossypium hirsutum) fibres consist of single cells that grow in a highly polarized manner, assumed to be controlled by the cytoskeleton(1-3). However, how the cytoskeletal organization and dynamics underpin fibre development remains unexplored. Moreover, it is unclear whether cotton fibres expand via tip growth or diffuse growth(2-4). We generated stable transgenic cotton plants expressing fluorescent markers of the actin and microtubule cytoskeleton. Live-cell imaging revealed that elongating cotton fibres assemble a cortical filamentous actin network that extends along the cell axis to finally form actin strands with closed loops in the tapered fibre tip. Analyses of F-actin network properties indicate that cotton fibres have a unique actin organization that blends features of both diffuse and tip growth modes. Interestingly, typical actin organization and endosomal vesicle aggregation found in tip-growing cell apices were not observed in fibre tips. Instead, endomembrane compartments were evenly distributed along the elongating fibre cells and moved bi-directionally along the fibre shank to the fibre tip. Moreover, plus-end tracked microtubules transversely encircled elongating fibre shanks, reminiscent of diffusely growing cells. Collectively, our findings indicate that cotton fibres elongate via a unique tip-biased diffuse growth mode.…
MetadatenAuthor details: | Yanjun Yu, Shenjie Wu, Jacqueline NowakORCiDGND, Guangda WangORCiD, Libo Han, Zhidi Feng, Amelie Mendrinna, Yinping Ma, Huan Wang, Xiaxia Zhang, Juan Tian, Li Dong, Zoran NikoloskiORCiDGND, Staffan PerssonORCiDGND, Zhaosheng KongORCiD |
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DOI: | https://doi.org/10.1038/s41477-019-0418-8 |
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ISSN: | 2055-026X |
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ISSN: | 2055-0278 |
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Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/31040442 |
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Title of parent work (English): | Nature plants |
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Publisher: | Nature Publ. Group |
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Place of publishing: | London |
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Publication type: | Article |
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Language: | English |
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Date of first publication: | 2019/04/30 |
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Publication year: | 2019 |
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Release date: | 2021/02/23 |
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Volume: | 5 |
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Issue: | 5 |
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Number of pages: | 7 |
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First page: | 498 |
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Last Page: | 504 |
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Funding institution: | National Key Research and Development Program of China [2016YFD0100505, 2016YFD0100306]; National Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of China [31601350]; National Major Project for Developing New GM Crops [2016ZX08005]; State Key Laboratory of Plant Genomics; R@MAP Professorship at University of Melbourne; Future Fellowship grant [FT160100218]; IRRTF-RNC grant via University of Melbourne |
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Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
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DDC classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
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Peer review: | Referiert |
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