A highly efficient pipeline for protein expression in Leishmania tarentolae using infrared fluorescence protein as marker
- Background: Leishmania tarentolae, a unicellular eukaryotic protozoan, has been established as a novel host for recombinant protein production in recent years. Current protocols for protein expression in Leishmania are, however, time consuming and require extensive lab work in order to identify well-expressing cell lines. Here we established an alternative protein expression work-flow that employs recently engineered infrared fluorescence protein (IFP) as a suitable and easy-to-handle reporter protein for recombinant protein expression in Leishmania. As model proteins we tested three proteins from the plant Arabidopsis thaliana, including a NAC and a type-B ARR transcription factor. Results: IFP and IFP fusion proteins were expressed in Leishmania and rapidly detected in cells by deconvolution microscopy and in culture by infrared imaging of 96-well microtiter plates using small cell culture volumes (2 mu L
Author details: | Hakan Dortay, Bernd Müller-RöberORCiDGND |
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URL: | http://www.microbialcellfactories.com/home/ |
DOI: | https://doi.org/10.1186/1475-2859-9-29 |
ISSN: | 1475-2859 |
Publication type: | Article |
Language: | English |
Year of first publication: | 2010 |
Publication year: | 2010 |
Release date: | 2017/03/25 |
Source: | Microbial cell factories. - ISSN 1475-2859. - 9 (2010), 5, Art. 29 |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
Peer review: | Referiert |
Publishing method: | Open Access |