Xi Wen, Viktoria Unger, Gerald Jurasinski, Franziska Koebsch, Fabian Horn, Gregor Rehder, Torsten Sachs, Dominik Zak, Gunnar Lischeid, Klaus-Holger Knorr, Michael E. Boettcher, Matthias Winkel, Paul L. E. Bodelier, Susanne Liebner
- The rewetting of drained peatlands alters peat geochemistry and often leads to sustained elevated methane emission. Although this methane is produced entirely by microbial activity, the distribution and abundance of methane-cycling microbes in rewetted peatlands, especially in fens, is rarely described. In this study, we compare the community composition and abundance of methane-cycling microbes in relation to peat porewater geochemistry in two rewetted fens in northeastern Germany, a coastal brackish fen and a freshwater riparian fen, with known high methane fluxes. We utilized 16S rRNA high-throughput sequencing and quantitative polymerase chain reaction (qPCR) on 16S rRNA, mcrA, and pmoA genes to determine microbial community composition and the abundance of total bacteria, methanogens, and methanotrophs. Electrical conductivity (EC) was more than 3 times higher in the coastal fen than in the riparian fen, averaging 5.3 and 1.5 mS cm(-1), respectively. Porewater concentrations of terminal electron acceptors (TEAs) varied within andThe rewetting of drained peatlands alters peat geochemistry and often leads to sustained elevated methane emission. Although this methane is produced entirely by microbial activity, the distribution and abundance of methane-cycling microbes in rewetted peatlands, especially in fens, is rarely described. In this study, we compare the community composition and abundance of methane-cycling microbes in relation to peat porewater geochemistry in two rewetted fens in northeastern Germany, a coastal brackish fen and a freshwater riparian fen, with known high methane fluxes. We utilized 16S rRNA high-throughput sequencing and quantitative polymerase chain reaction (qPCR) on 16S rRNA, mcrA, and pmoA genes to determine microbial community composition and the abundance of total bacteria, methanogens, and methanotrophs. Electrical conductivity (EC) was more than 3 times higher in the coastal fen than in the riparian fen, averaging 5.3 and 1.5 mS cm(-1), respectively. Porewater concentrations of terminal electron acceptors (TEAs) varied within and among the fens. This was also reflected in similarly high intra- and inter-site variations of microbial community composition. Despite these differences in environmental conditions and electron acceptor availability, we found a low abundance of methanotrophs and a high abundance of methanogens, represented in particular by Methanosaetaceae, in both fens. This suggests that rapid (re) establishment of methanogens and slow (re) establishment of methanotrophs contributes to prolonged increased methane emissions following rewetting.…
MetadatenAuthor details: | Xi Wen, Viktoria UngerORCiD, Gerald JurasinskiORCiD, Franziska Koebsch, Fabian HornORCiD, Gregor RehderORCiD, Torsten SachsORCiDGND, Dominik ZakORCiD, Gunnar LischeidORCiDGND, Klaus-Holger KnorrORCiD, Michael E. Boettcher, Matthias WinkelORCiDGND, Paul L. E. Bodelier, Susanne LiebnerORCiDGND |
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DOI: | https://doi.org/10.5194/bg-15-6519-2018 |
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ISSN: | 1726-4170 |
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ISSN: | 1726-4189 |
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Title of parent work (English): | Biogeosciences |
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Publisher: | Copernicus |
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Place of publishing: | Göttingen |
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Publication type: | Article |
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Language: | English |
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Date of first publication: | 2018/11/06 |
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Publication year: | 2018 |
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Release date: | 2021/06/30 |
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Volume: | 15 |
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Issue: | 21 |
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Number of pages: | 18 |
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First page: | 6519 |
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Last Page: | 6536 |
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Funding institution: | DFG (Deutsche Forschungsgemeinschaft)German Research Foundation (DFG) [GRK 2000]; China Scholarship Council (CSC)China Scholarship Council [201408620031]; National Science Foundation (NSF)National Science Foundation (NSF) [ARCSS-1500931]; Helmholtz Gemeinschaft (HGF)Helmholtz Association [VH-NG-919, VH-NG-821]; Helmholtz Postdoc Programme [PD-129]; Terrestrial Environmental Observatories (TERENO) Network; Leibniz Institute for Baltic Sea Research (IOW); European Social Fund (ESF)European Social Fund (ESF) [ESF/14-BM-A55-0030/16]; Ministry of Education, Science and Culture of Mecklenburg-Western Pomerania [ESF/14-BM-A55-0030/16] |
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Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
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DDC classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
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License (German): | CC-BY - Namensnennung 4.0 International |
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