Direct determination of bacterial cell viability using carbon nanotubes modified screen-printed electrodes
- For the early detection of bacterial infection, there is a need for rapid, sensitive, and label-free assays. Thus, in this study, nanostrucured microbial electrochemical platform is designed to monitor the viability and cell growth of S. aureus. Using multi-walled carbon nanotube modified screen-printed electrodes (MWCNTs/SPE), the cyclic voltammetric measurements showed only one irreversible oxidation peak at 600 mV vs Ag/AgCl that accounts for the viable and metabolically active bacterial cells. The assay was optimized and the secreted metabolites, in the extracellular matrix, were directly detected. The peak current showed a positive correlation with viable cell numbers ranging from OD600 nm of 0.1 to 1.1, indicating that the activity of live cells can be quantified. Consequently, responses of viable and non-viable cells of S. aureus to the effects of antibiotic and respiratory chain inhibitors were determined. Thus, the proposed nanostructure-based bacterial sensor provides a reasonable and reliable way for real-time monitoring ofFor the early detection of bacterial infection, there is a need for rapid, sensitive, and label-free assays. Thus, in this study, nanostrucured microbial electrochemical platform is designed to monitor the viability and cell growth of S. aureus. Using multi-walled carbon nanotube modified screen-printed electrodes (MWCNTs/SPE), the cyclic voltammetric measurements showed only one irreversible oxidation peak at 600 mV vs Ag/AgCl that accounts for the viable and metabolically active bacterial cells. The assay was optimized and the secreted metabolites, in the extracellular matrix, were directly detected. The peak current showed a positive correlation with viable cell numbers ranging from OD600 nm of 0.1 to 1.1, indicating that the activity of live cells can be quantified. Consequently, responses of viable and non-viable cells of S. aureus to the effects of antibiotic and respiratory chain inhibitors were determined. Thus, the proposed nanostructure-based bacterial sensor provides a reasonable and reliable way for real-time monitoring of live-dead cell functions, and antibacterial profiling.…
| Author details: | Rabeay Y. A. HassanORCiD, Ulla WollenbergerORCiDGND |
|---|---|
| DOI: | https://doi.org/10.1002/elan.201900047 |
| ISSN: | 1040-0397 |
| ISSN: | 1521-4109 |
| Title of parent work (English): | Electroanalysis : an international journal devoted to fundamental and practical aspects of electroanalysis |
| Publisher: | Wiley-VCH |
| Place of publishing: | Weinheim |
| Publication type: | Article |
| Language: | English |
| Date of first publication: | 2019/05/21 |
| Publication year: | 2019 |
| Release date: | 2021/01/28 |
| Tag: | Bacterial biosensor; carbon nanotubes (CNTs); cell viability assay; screen printed electrodes (SPEs) |
| Volume: | 31 |
| Issue: | 6 |
| Number of pages: | 6 |
| First page: | 1112 |
| Last Page: | 1117 |
| Funding institution: | Egyptian Ministry of Higher Education and Scientific Research; German Academic Exchange Service (DAAD, Cairo, Egypt) via the German Egyptian Short Term Scholarship (GERSS program) |
| Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
| DDC classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
| Peer review: | Referiert |
