Bioelectrocatalytic Reduction of Hydrogen Peroxide by Microperoxidase-11 Immobilized on Mesoporous Antimony-Doped Tin Oxide
- The heme-undecapeptide microperoxidase-11 (MP-11) was immobilized on mesoporous antimony-doped tin oxide (ATO) thin-film electrodes modified with the positively charged binding promotor polydiallyldimethylammonium chloride. Surface concentrations of MP-11 of 1.5 nmol cm(-2) were sufficiently high to enable spectroelectrochemical analyses. UV/Vis spectroscopy and resonance Raman spectroscopy revealed that immobilized MP-11 adopts a six-coordinated low-spin conformation, as in solution in the presence of a polycation. Cathodic reduction of hydrogen peroxide at potentials close to +500mV versus Ag/AgCl indicates that the reaction proceeds via a Compound I-type like intermediate, analogous to natural peroxidases, and confirms mesoporous ATO as a suitable host material for adsorbing the heme-peptide in its native state. A hydrogen peroxide sensor is proposed by using the bioelectrocatalytic properties of the MP-11-modified ATO.
Author details: | Bettina NeumannORCiD, Patrycja Kielb, Lina Rustam, Anna Fischer, Inez M. Weidinger, Ulla WollenbergerORCiDGND |
---|---|
DOI: | https://doi.org/10.1002/celc.201600776 |
ISSN: | 2196-0216 |
Title of parent work (English): | ChemElectrChem |
Publisher: | Wiley-VCH |
Place of publishing: | Weinheim |
Publication type: | Article |
Language: | English |
Date of first publication: | 2017/01/11 |
Publication year: | 2017 |
Release date: | 2022/06/13 |
Tag: | electrochemistry; enzyme catalysis; mesoporous materials; microperoxidase; spectroelectrochemistry |
Volume: | 4 |
Issue: | 4 |
Number of pages: | 7 |
First page: | 913 |
Last Page: | 919 |
Funding institution: | Unicat Cluster of Excellence (Deutsche Forschungsgemeinschaft) [EXC 314/2]; Albert-Ludwigs-University Freiburg |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
DDC classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Peer review: | Referiert |