Bioelectrocatalytic Reduction of Hydrogen Peroxide by Microperoxidase-11 Immobilized on Mesoporous Antimony-Doped Tin Oxide
- The heme-undecapeptide microperoxidase-11 (MP-11) was immobilized on mesoporous antimony-doped tin oxide (ATO) thin-film electrodes modified with the positively charged binding promotor polydiallyldimethylammonium chloride. Surface concentrations of MP-11 of 1.5 nmol cm(-2) were sufficiently high to enable spectroelectrochemical analyses. UV/Vis spectroscopy and resonance Raman spectroscopy revealed that immobilized MP-11 adopts a six-coordinated low-spin conformation, as in solution in the presence of a polycation. Cathodic reduction of hydrogen peroxide at potentials close to +500mV versus Ag/AgCl indicates that the reaction proceeds via a Compound I-type like intermediate, analogous to natural peroxidases, and confirms mesoporous ATO as a suitable host material for adsorbing the heme-peptide in its native state. A hydrogen peroxide sensor is proposed by using the bioelectrocatalytic properties of the MP-11-modified ATO.
Verfasserangaben: | Bettina NeumannORCiD, Patrycja Kielb, Lina Rustam, Anna Fischer, Inez M. Weidinger, Ulla WollenbergerORCiDGND |
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DOI: | https://doi.org/10.1002/celc.201600776 |
ISSN: | 2196-0216 |
Titel des übergeordneten Werks (Englisch): | ChemElectrChem |
Verlag: | Wiley-VCH |
Verlagsort: | Weinheim |
Publikationstyp: | Wissenschaftlicher Artikel |
Sprache: | Englisch |
Datum der Erstveröffentlichung: | 11.01.2017 |
Erscheinungsjahr: | 2017 |
Datum der Freischaltung: | 13.06.2022 |
Freies Schlagwort / Tag: | electrochemistry; enzyme catalysis; mesoporous materials; microperoxidase; spectroelectrochemistry |
Band: | 4 |
Ausgabe: | 4 |
Seitenanzahl: | 7 |
Erste Seite: | 913 |
Letzte Seite: | 919 |
Fördernde Institution: | Unicat Cluster of Excellence (Deutsche Forschungsgemeinschaft) [EXC 314/2]; Albert-Ludwigs-University Freiburg |
Organisationseinheiten: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
DDC-Klassifikation: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Peer Review: | Referiert |