Parallel generation and detection of superoxide and hydrogen peroxide in a fluidic chip
- A fluidic chip system was developed, which combines a stable generation of superoxide radicals and hydrogen peroxide with their sensorial detection. The generation of both reactive oxygen species was achieved by immobilization of xanthine oxidase on controlled pore glass in a reaction chamber. Antioxidants can be introduced into the fluidic chip system by means of mixing chamber. The detection of both species is based on the amperometric principle using a biosensor chip with two working electrodes. As sensing protein for both electrodes cytochrome c was used. The novel system was designed for the quantification of the antioxidant efficiency of different potential scavengers of the respective reactive species in an aqueous medium. Several model antioxidants such as ascorbic acid or catalase have been tested under flow conditions.
Verfasserangaben: | Andrey. V. Krylov, H. Adamzig, A. D. Walter, B. Loechel, E. Kurth, O. Pulz, Jan SzeponikGND, Franziska Wegerich, Fred LisdatORCiDGND |
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DOI: | https://doi.org/10.1016/j.snb.2005.11.062 |
ISSN: | 0925-4005 |
Titel des übergeordneten Werks (Englisch): | Sensors and actuators : B, Chemical |
Verlag: | Elsevier |
Verlagsort: | Lausanne |
Publikationstyp: | Wissenschaftlicher Artikel |
Sprache: | Englisch |
Datum der Erstveröffentlichung: | 24.11.2006 |
Erscheinungsjahr: | 2006 |
Datum der Freischaltung: | 21.04.2020 |
Freies Schlagwort / Tag: | antioxidant; biosensor; cytochrome c; flow system; reactive oxygen species |
Band: | 119 |
Ausgabe: | 1 |
Seitenanzahl: | 9 |
Erste Seite: | 118 |
Letzte Seite: | 126 |
Organisationseinheiten: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
DDC-Klassifikation: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Peer Review: | Referiert |