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Morphogenetic control of zebrafish cardiac looping by Bmp signaling
- Cardiac looping is an essential and highly conserved morphogenetic process that places the different regions of the developing vertebrate heart tube into proximity of their final topographical positions. High-resolution 4D live imaging of mosaically labelled cardiomyocytes reveals distinct cardiomyocyte behaviors that contribute to the deformation of the entire heart tube. Cardiomyocytes acquire a conical cell shape, which is most pronounced at the superior wall of the atrioventricular canal and contributes to S-shaped bending. Torsional deformation close to the outflow tract contributes to a torque-like winding of the entire heart tube between its two poles. Anisotropic growth of cardiomyocytes based on their positions reinforces S-shaping of the heart. During cardiac looping, bone morphogenetic protein pathway signaling is strongest at the future superior wall of the atrioventricular canal. Upon pharmacological or genetic inhibition of bone morphogenetic protein signaling, myocardial cells at the superior wall of theCardiac looping is an essential and highly conserved morphogenetic process that places the different regions of the developing vertebrate heart tube into proximity of their final topographical positions. High-resolution 4D live imaging of mosaically labelled cardiomyocytes reveals distinct cardiomyocyte behaviors that contribute to the deformation of the entire heart tube. Cardiomyocytes acquire a conical cell shape, which is most pronounced at the superior wall of the atrioventricular canal and contributes to S-shaped bending. Torsional deformation close to the outflow tract contributes to a torque-like winding of the entire heart tube between its two poles. Anisotropic growth of cardiomyocytes based on their positions reinforces S-shaping of the heart. During cardiac looping, bone morphogenetic protein pathway signaling is strongest at the future superior wall of the atrioventricular canal. Upon pharmacological or genetic inhibition of bone morphogenetic protein signaling, myocardial cells at the superior wall of the atrioventricular canal maintain cuboidal cell shapes and S-shaped bending is impaired. This description of cellular rearrangements and cardiac looping regulation may also be relevant for understanding the etiology of human congenital heart defects.…
Author details: | Verónica A. LombardoORCiD, Melina HeiseGND, Motahareh MoghtadaeiORCiD, Dorothee BornhorstORCiDGND, Jörg MännerORCiD, Salim Abdelilah-SeyfriedORCiDGND |
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DOI: | https://doi.org/10.1242/dev.180091 |
ISSN: | 0950-1991 |
ISSN: | 1477-9129 |
Pubmed ID: | https://pubmed.ncbi.nlm.nih.gov/31628109 |
Title of parent work (English): | Development : Company of Biologists |
Publisher: | The Company of Biologists Ltd |
Place of publishing: | Cambridge |
Publication type: | Article |
Language: | English |
Year of first publication: | 2019 |
Publication year: | 2019 |
Release date: | 2020/10/06 |
Tag: | BMP; Cardiac looping; Hemodynamics; Wnt; Zebrafish |
Volume: | 146 |
Issue: | 22 |
Number of pages: | 13 |
Funding institution: | Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET)Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET); Excellence cluster REBIRTH; Joachim Herz Stiftung; Deutsche ForschungsgemeinschaftGerman Research Foundation (DFG) [SE2016/7-2, SE2016/10-1]; Deutsche Zentrum fur Herz-Kreislauf-Forschung [SFB958] |
Organizational units: | Mathematisch-Naturwissenschaftliche Fakultät / Institut für Biochemie und Biologie |
DDC classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Peer review: | Referiert |